Hydrocarbonoclastic Biofilm-Based Microbial Fuel Cells: Exploiting Biofilms at Water-Oil Interface for Renewable Energy and Wastewater Remediation.

IF 4.9 3区 工程技术 Q1 CHEMISTRY, ANALYTICAL Biosensors-Basel Pub Date : 2024-10-08 DOI:10.3390/bios14100484
Nicola Lovecchio, Roberto Giuseppetti, Lucia Bertuccini, Sandra Columba-Cabezas, Valentina Di Meo, Mario Figliomeni, Francesca Iosi, Giulia Petrucci, Michele Sonnessa, Fabio Magurano, Emilio D'Ugo
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Abstract

Microbial fuel cells (MFCs) represent a promising technology for sustainable energy generation, which leverages the metabolic activities of microorganisms to convert organic substrates into electrical energy. In oil spill scenarios, hydrocarbonoclastic biofilms naturally form at the water-oil interface, creating a distinct environment for microbial activity. In this work, we engineered a novel MFC that harnesses these biofilms by strategically positioning the positive electrode at this critical junction, integrating the biofilm's natural properties into the MFC design. These biofilms, composed of specialized hydrocarbon-degrading bacteria, are vital in supporting electron transfer, significantly enhancing the system's power generation. Next-generation sequencing and scanning electron microscopy were used to characterize the microbial community, revealing a significant enrichment of hydrocarbonoclastic Gammaproteobacteria within the biofilm. Notably, key genera such as Paenalcaligenes, Providencia, and Pseudomonas were identified as dominant members, each contributing to the degradation of complex hydrocarbons and supporting the electrogenic activity of the MFCs. An electrochemical analysis demonstrated that the MFC achieved a stable power output of 51.5 μW under static conditions, with an internal resistance of about 1.05 kΩ. The system showed remarkable long-term stability, which maintained consistent performance over a 5-day testing period, with an average daily energy storage of approximately 216 mJ. Additionally, the MFC effectively recovered after deep discharge cycles, sustaining power output for up to 7.5 h before requiring a recovery period. Overall, the study indicates that MFCs based on hydrocarbonoclastic biofilms provide a dual-functionality system, combining renewable energy generation with environmental remediation, particularly in wastewater treatment. Despite lower power output compared to other hydrocarbon-degrading MFCs, the results highlight the potential of this technology for autonomous sensor networks and other low-power applications, which required sustainable energy sources. Moreover, the hydrocarbonoclastic biofilm-based MFC presented here offer significant potential as a biosensor for real-time monitoring of hydrocarbons and other contaminants in water. The biofilm's electrogenic properties enable the detection of organic compound degradation, positioning this system as ideal for environmental biosensing applications.

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基于碳氢化合物生物膜的微生物燃料电池:利用水油界面的生物膜实现可再生能源和废水修复。
微生物燃料电池(MFC)是一种前景广阔的可持续能源发电技术,它利用微生物的新陈代谢活动将有机基质转化为电能。在溢油情况下,水油界面会自然形成碳氢化合物生物膜,为微生物活动创造了独特的环境。在这项工作中,我们设计了一种新型 MFC,通过将正电极战略性地放置在这一关键交界处,将生物膜的自然特性融入 MFC 设计中,从而利用这些生物膜。这些生物膜由专门的碳氢化合物降解细菌组成,对支持电子传递至关重要,从而大大提高了系统的发电量。下一代测序技术和扫描电子显微镜被用来描述微生物群落的特征,结果表明生物膜中碳氢化合物分解噬菌体(hydrocarbonoclastic Gammaproteobacteria)大量富集。值得注意的是,Paenalcaligenes、Providencia 和 Pseudomonas 等关键菌属被确定为主要成员,它们都有助于降解复杂的碳氢化合物,并支持 MFC 的电生活性。电化学分析表明,MFC 在静态条件下可稳定输出 51.5 μW 的功率,内阻约为 1.05 kΩ。该系统显示出卓越的长期稳定性,在为期 5 天的测试期间保持了稳定的性能,平均每天的能量储存约为 216 mJ。此外,MFC 还能在深度放电循环后有效恢复,在需要恢复期之前维持功率输出长达 7.5 小时。总之,这项研究表明,基于碳氢化合物生物膜的 MFC 是一种具有双重功能的系统,可将可再生能源发电与环境修复相结合,特别是在废水处理方面。尽管与其他碳氢化合物降解 MFC 相比,该技术的功率输出较低,但研究结果凸显了该技术在自主传感器网络和其他需要可持续能源的低功率应用中的潜力。此外,本文介绍的基于碳氢化合物生物膜的 MFC 作为一种生物传感器,在实时监测水中碳氢化合物和其他污染物方面具有巨大潜力。生物膜的电生特性能够检测有机化合物的降解,使该系统成为环境生物传感应用的理想选择。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biosensors-Basel
Biosensors-Basel Biochemistry, Genetics and Molecular Biology-Clinical Biochemistry
CiteScore
6.60
自引率
14.80%
发文量
983
审稿时长
11 weeks
期刊介绍: Biosensors (ISSN 2079-6374) provides an advanced forum for studies related to the science and technology of biosensors and biosensing. It publishes original research papers, comprehensive reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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