Microfluidic Detection Platform for Determination of Ractopamine in Food.

IF 4.9 3区工程技术 Q1 CHEMISTRY, ANALYTICAL Biosensors-Basel Pub Date : 2024-09-26 DOI:10.3390/bios14100462

Cheng-Xue Yu, Kuan-Hsun Huang, To-Lin Chen, Chan-Chiung Liu, Lung-Ming Fu

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Abstract

A novel microfluidic ractopamine (RAC) detection platform consisting of a microfluidic RAC chip and a smart analysis device is proposed for the determination of RAC concentration in meat samples. This technology utilizes gold nanoparticles (AuNPs) modified with glutamic acid (GLU) and polyethyleneimine (PEI) to measure RAC concentration in food products. When RAC is present, AuNPs aggregate through hydrogen bonding, causing noticeable changes in their optical properties, which are detected using a self-built UV-visible micro-spectrophotometer. Within the range of 5 to 80 ppb, a linear relationship exists between the absorbance ratio (A_693nm/A_518nm) (Y) and RAC concentration (X), expressed as Y = 0.0054X + 0.4690, with a high coefficient of determination (R² = 0.9943). This method exhibits a detection limit of 1.0 ppb and achieves results within 3 min. The practical utility of this microfluidic assay is exemplified through the evaluation of RAC concentrations in 50 commercially available meat samples. The variance between concentrations measured using this platform and those determined via liquid chromatography-tandem mass spectrometry (LC-MS/MS) is less than 8.33%. These results underscore the viability of the microfluidic detection platform as a rapid and cost-effective solution for ensuring food safety and regulatory compliance within the livestock industry.

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用于检测食品中莱克多巴胺的微流控检测平台

本研究提出了一种新型微流控莱克多巴胺（RAC）检测平台，该平台由微流控莱克多巴胺芯片和智能分析装置组成，用于测定肉类样品中的莱克多巴胺浓度。该技术利用谷氨酸（GLU）和聚乙烯亚胺（PEI）修饰的金纳米粒子（AuNPs）来检测食品中的莱克多巴胺浓度。当食品中含有 RAC 时，AuNPs 会通过氢键聚合，从而导致其光学特性发生明显变化，并通过自建的紫外可见微分光光度计进行检测。在 5 至 80 ppb 的范围内，吸光度比值（A693nm/A518nm）（Y）与 RAC 浓度（X）之间存在线性关系，即 Y = 0.0054X + 0.4690，且测定系数较高（R2 = 0.9943）。该方法的检测限为 1.0 ppb，可在 3 分钟内得出结果。通过对 50 份市售肉类样品中的 RAC 浓度进行评估，证明了这种微流控检测方法的实用性。使用该平台测量的浓度与液相色谱-串联质谱法（LC-MS/MS）测定的浓度之间的差异小于 8.33%。这些结果凸显了微流控检测平台的可行性，它是一种快速、经济的解决方案，可确保畜牧业的食品安全和合规性。

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来源期刊

Biosensors-Basel Biochemistry, Genetics and Molecular Biology-Clinical Biochemistry

CiteScore

6.60

自引率

14.80%

发文量

983

审稿时长

11 weeks

期刊介绍： Biosensors (ISSN 2079-6374) provides an advanced forum for studies related to the science and technology of biosensors and biosensing. It publishes original research papers, comprehensive reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.