Visualizing Macrophage Polarization through Fluorescent mRNA Profiling.

IF 4.9 3区 工程技术 Q1 CHEMISTRY, ANALYTICAL Biosensors-Basel Pub Date : 2024-10-02 DOI:10.3390/bios14100475
Miaomiao Xu, Siyuan Wei, Tong Su, Die Ma, Zhixuan Wang, Dan Zhu, Lixing Weng, Xianguang Ding
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Abstract

Macrophages, known for their phenotypic plasticity, play a critical role in maintaining homeostasis and inflammation-related pathogenesis. Although identifying diverse macrophage phenotypes holds promise for enhancing diagnoses and treatments of diseases mediated by macrophages, existing methodologies for differentiating macrophages often lack precision. They are limited by the cumbersome procedures that require large-scale equipment, such as flow cytometry and transcriptomic analysis. In this context, we have engineered fluorescent polyadenine (polyA)-mediated sticky flares that enable practical visualization of macrophages. This technology facilitates the highly sensitive detection of macrophage phenotypes through the specific recognition of intracellular mRNAs, permitting in situ imaging. Our approach demonstrates the potential for determining macrophage polarization status at the single-cell level within dynamic immune microenvironments, thereby providing crucial diagnostic and prognostic information that could guide the development of tailored treatments for macrophage-related diseases in personalized medicine.

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通过荧光 mRNA 图谱对巨噬细胞极化进行可视化分析
巨噬细胞以其表型可塑性而闻名,在维持体内平衡和炎症相关发病机制中发挥着关键作用。尽管识别不同的巨噬细胞表型有望提高对由巨噬细胞介导的疾病的诊断和治疗水平,但现有的巨噬细胞分化方法往往缺乏精确性。它们受限于需要大型设备的繁琐程序,如流式细胞仪和转录组分析。在这种情况下,我们设计了由荧光聚腺嘌呤(polyA)介导的粘性耀斑,使巨噬细胞的可视化成为可能。这项技术通过特异性识别细胞内 mRNA,实现了对巨噬细胞表型的高灵敏度检测,并允许原位成像。我们的方法展示了在动态免疫微环境中单细胞水平上确定巨噬细胞极化状态的潜力,从而提供重要的诊断和预后信息,为开发个性化医疗中巨噬细胞相关疾病的定制治疗方法提供指导。
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来源期刊
Biosensors-Basel
Biosensors-Basel Biochemistry, Genetics and Molecular Biology-Clinical Biochemistry
CiteScore
6.60
自引率
14.80%
发文量
983
审稿时长
11 weeks
期刊介绍: Biosensors (ISSN 2079-6374) provides an advanced forum for studies related to the science and technology of biosensors and biosensing. It publishes original research papers, comprehensive reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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