Targeted modulation of pennycress lipid droplet proteins impacts droplet morphology and seed oil content.

IF 6.2 1区 生物学 Q1 PLANT SCIENCES The Plant Journal Pub Date : 2024-10-28 DOI:10.1111/tpj.17109
Athanas Guzha, Barsanti Gautam, Damiano Marchiafava, Julius Ver Sagun, Tatiana Garcia, Brice A Jarvis, Allison M Barbaglia-Hurlock, Christopher Johnston, Erich Grotewold, John C Sedbrook, Ana Paula Alonso, Kent D Chapman
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Abstract

Lipid droplets (LDs) are unusual organelles that have a phospholipid monolayer surface and a hydrophobic matrix. In oilseeds, this matrix is nearly always composed of triacylglycerols (TGs) for efficient storage of carbon and energy. Various proteins play a role in their assembly, stability and turnover, and even though the major structural oleosin proteins in seed LDs have been known for decades, the factors influencing LD formation and dynamics are still being uncovered mostly in the "model oilseed" Arabidopsis. Here we identified several key LD biogenesis proteins in the seeds of pennycress, a potential biofuel crop, that were correlated previously with seed oil content and characterized here for their participation in LD formation in transient expression assays and stable transgenics. One pennycress protein, the lipid droplet associated protein-interacting protein (LDIP), was able to functionally complement the Arabidopsis ldip mutant, emphasizing the close conservation of lipid storage among these two Brassicas. Moreover, loss-of-function ldip mutants in pennycress exhibited increased seed oil content without compromising plant growth, raising the possibility that LDIP or other LD biogenesis factors may be suitable targets for improving yields in oilseed crops more broadly.

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靶向调节菥蓂脂滴蛋白可影响脂滴形态和种子含油量。
脂滴(LDs)是一种不寻常的细胞器,具有磷脂单层表面和疏水基质。在油籽中,这种基质几乎总是由三酰甘油(TGs)组成,用于有效储存碳和能量。尽管种子 LD 中的主要结构性油脂素蛋白已经存在了几十年,但影响 LD 形成和动态的因素仍主要是在 "油籽模型 "拟南芥中发现的。在这里,我们在菥蓂(一种潜在的生物燃料作物)的种子中发现了几种关键的 LD 生物发生蛋白,这些蛋白以前与种子含油量相关,现在则在瞬时表达试验和稳定转基因中鉴定了它们参与 LD 形成的特性。其中一种菥蓂属蛋白--脂滴相关蛋白相互作用蛋白(LDIP)--能够对拟南芥的ldip突变体进行功能互补,强调了这两种十字花科植物在脂质储存方面的密切保护。此外,菥蓂属植物的功能缺失ldip突变体在不影响植物生长的情况下提高了种子含油量,这就使LDIP或其他LD生物发生因子有可能成为更广泛地提高油料作物产量的合适靶标。
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来源期刊
The Plant Journal
The Plant Journal 生物-植物科学
CiteScore
13.10
自引率
4.20%
发文量
415
审稿时长
2.3 months
期刊介绍: Publishing the best original research papers in all key areas of modern plant biology from the world"s leading laboratories, The Plant Journal provides a dynamic forum for this ever growing international research community. Plant science research is now at the forefront of research in the biological sciences, with breakthroughs in our understanding of fundamental processes in plants matching those in other organisms. The impact of molecular genetics and the availability of model and crop species can be seen in all aspects of plant biology. For publication in The Plant Journal the research must provide a highly significant new contribution to our understanding of plants and be of general interest to the plant science community.
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