Reference Genes for the Real-Time PCR Analysis of Relative Gene Expression in Various Human Myocardial Pathologies

IF 0.8 Q3 Engineering Nanotechnologies in Russia Pub Date : 2024-10-29 DOI:10.1134/S263516762460113X

A. L. Klass, A. Kh. Aliyeva, M. M. Rudenok, A. V. Lysenko, G. I. Salagaev, M. I. Shadrina, P. A. Slominsky, E. V. Filatova

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Abstract

Active study of the molecular mechanisms of the occurrence and development of various cardiac pathologies using the assessment of gene expression by real-time PCR is necessary to solve the problem of finding and selecting adequate reference genes. A search is conducted for the most stably expressed potential reference genes in the intravital myocardial samples of patients with severe heart failure due to hypertrophic cardiomyopathy or coronary heart disease. Gene-expression analysis is performed using reverse transcription and real-time PCR. Gene-expression stability is assessed using the RefFinder resource. It is shown that the genes expressed most stably in human myocardium in the case of heart failure are PSMD6, PSMD7, SARS1, and AARS1. In this regard, we recommend including them in the gene panel when searching for and validating potential reference genes to assess the expression of candidate genes in myocardial tissues.

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用于实时 PCR 分析各种人类心肌病变中相对基因表达的参考基因

利用实时 PCR 评估基因表达，积极研究各种心脏病变发生和发展的分子机制，是解决寻找和选择适当参考基因问题的必要条件。在肥厚型心肌病或冠心病导致的严重心力衰竭患者的心肌内样本中寻找表达最稳定的潜在参考基因。基因表达分析采用反转录和实时 PCR 技术进行。使用 RefFinder 资源评估基因表达的稳定性。结果表明，在心力衰竭情况下，人类心肌中表达最稳定的基因是 PSMD6、PSMD7、SARS1 和 AARS1。因此，我们建议在搜索和验证潜在参考基因以评估候选基因在心肌组织中的表达时，将它们纳入基因面板。

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来源期刊

Nanotechnologies in Russia NANOSCIENCE & NANOTECHNOLOGY-

CiteScore

1.20

自引率

0.00%

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期刊介绍： Nanobiotechnology Reports publishes interdisciplinary research articles on fundamental aspects of the structure and properties of nanoscale objects and nanomaterials, polymeric and bioorganic molecules, and supramolecular and biohybrid complexes, as well as articles that discuss technologies for their preparation and processing, and practical implementation of products, devices, and nature-like systems based on them. The journal publishes original articles and reviews that meet the highest scientific quality standards in the following areas of science and technology studies: self-organizing structures and nanoassemblies; nanostructures, including nanotubes; functional and structural nanomaterials; polymeric, bioorganic, and hybrid nanomaterials; devices and products based on nanomaterials and nanotechnology; nanobiology and genetics, and omics technologies; nanobiomedicine and nanopharmaceutics; nanoelectronics and neuromorphic computing systems; neurocognitive systems and technologies; nanophotonics; natural science methods in a study of cultural heritage items; metrology, standardization, and monitoring in nanotechnology.