Ethylamine, beyond the synthetic precursor of theanine: CsCBF4-CsAlaDC module promoted ethylamine synthesis to enhance osmotic tolerance in tea plants.

IF 6.2 1区 生物学 Q1 PLANT SCIENCES The Plant Journal Pub Date : 2024-10-30 DOI:10.1111/tpj.17089
Ziwen Zhou, Xiangzong Luo, Maoyin Fu, Siya Li, Yaohua Cheng, Yeyun Li, Xianchen Zhang
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Abstract

The tea plant (Camellia sinensis) is a perennial green plant, and its tender leaves are rich in secondary metabolites, such as theanine. Ethylamine (EA), a small amine, is an important prerequisite for theanine synthesis. However, beyond its involvement in theanine synthesis, the other physiological functions of EA in tea plants remain unknown. In vitro experiments indicate that EA may function as scavengers of reactive oxygen species (ROS) to protect the plant against damage caused by osmotic stress. Additionally, a significant correlation between EA levels and osmotic tolerance has been observed in different tea varieties. From the results, alanine decarboxylase (CsAlaDC)-silenced tea leaves and overexpressed CsAlaDC Arabidopsis thaliana lines decreased and increased EA levels, respectively, and mediated ROS homeostasis, thus exhibiting a sensitive and tolerant phenotype. In addition, the transcription factor (TF) CsCBF4 was functionally identified, which can directly bind to the CsAlaDC promoter. CsCBF4-silenced tea leaves significantly reduced the expression levels of CsAlaDC and in turn EA content, resulting in excess ROS accumulation and an osmotic-sensitive phenotype. Taken together, these results established a new regulatory module consisting of CBF4-CsAlaDC responsible for EA accumulation and ROS homeostasis in response to osmotic stress.

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乙胺是茶氨酸的合成前体:CsCBF4-CsAlaDC模块促进乙胺合成,增强茶树的耐渗透性。
茶树(Camellia sinensis)是一种多年生绿色植物,其嫩叶富含茶氨酸等次级代谢产物。乙胺(EA)是一种小胺,是合成茶氨酸的重要前提。然而,除了参与茶氨酸的合成外,EA 在茶树中的其他生理功能仍然未知。体外实验表明,EA 可作为活性氧(ROS)的清除剂,保护植物免受渗透胁迫造成的损害。此外,在不同的茶叶品种中观察到 EA 水平与渗透耐受性之间存在明显的相关性。结果显示,丙氨酸脱羧酶(CsAlaDC)沉默的茶叶和过表达 CsAlaDC 的拟南芥品系分别降低和提高了 EA 水平,并介导了 ROS 的平衡,从而表现出敏感和耐受的表型。此外,还发现了转录因子(TF)CsCBF4,它能直接与 CsAlaDC 启动子结合。被 CsCBF4 沉默的茶叶会显著降低 CsAlaDC 的表达水平,进而降低 EA 含量,导致过量的 ROS 积累和渗透敏感表型。综上所述,这些结果建立了一个由 CBF4-CsAlaDC 组成的新调控模块,该模块负责 EA 积累和 ROS 平衡,以应对渗透胁迫。
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来源期刊
The Plant Journal
The Plant Journal 生物-植物科学
CiteScore
13.10
自引率
4.20%
发文量
415
审稿时长
2.3 months
期刊介绍: Publishing the best original research papers in all key areas of modern plant biology from the world"s leading laboratories, The Plant Journal provides a dynamic forum for this ever growing international research community. Plant science research is now at the forefront of research in the biological sciences, with breakthroughs in our understanding of fundamental processes in plants matching those in other organisms. The impact of molecular genetics and the availability of model and crop species can be seen in all aspects of plant biology. For publication in The Plant Journal the research must provide a highly significant new contribution to our understanding of plants and be of general interest to the plant science community.
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