Laccase mimetics as sensing elements for amperometric assay of 5-hydroxyindoleacetic acid in urine

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Bioelectrochemistry Pub Date : 2024-10-24 DOI:10.1016/j.bioelechem.2024.108839

Olha Demkiv , Wojciech Nogala , Nataliya Stasyuk , Marcin Holdynski , Nina Dimcheva , Taras Danysh , Monika Asztemborska , Mykhailo Gonchar

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Abstract

Monitoring of the levels of 5-hydroxyindole-3-acetic acid (5-HIAA) is of significant importance for diagnostics of carcinoid tumors. We propose simple catalytic electrochemical sensors for the determination of 5-HIAA in urine using laccase and its mimetics. Laccase-like nanozymes (LacNZs) were synthesized via a chemical reduction, and resulting PtMn and MnO₂ nanoflowers (NFs) demonstrated laccase-like activity similar to the laccase from the Trametes zonata. In addition, these LacNZs showed enhanced stability under a wide range of pH (3.0–7.5), temperatures (4–70 °C), and ionic strengths (up to 500 mM NaCl). The developed PtMn NF/graphite electrode, similar to a laccase/graphite electrode, can detect 5-HIAA with a high sensitivity (25 000 ± 12 A·M⁻¹·m⁻² and 1900 ± 9 A·M⁻¹·m⁻², respectively) and have linear ranges of 0.3 – 15 μM and 2 – 50 μM. The sensors work at low working potentials with a detection limit of 0.16 and 1.4 μM, covering the normal and pathologic ranges of 5-HIAA (1 – 50 μM) content in urine. They have been successfully applied to 5-HIAA assay in urine samples of people with various diseases and revealed good recovery values and reproducibility. Additionally, the LacNZ-sensor has the best stability and can be used up to 20 days.

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将漆酶模拟物作为安培法测定尿液中 5-羟基吲哚乙酸的传感元件。

监测 5- 羟基吲哚-3-乙酸（5-HIAA）的水平对于类癌的诊断具有重要意义。我们提出了利用漆酶及其模拟物测定尿液中 5-HIAA 的简单催化电化学传感器。我们通过化学还原法合成了类似漆酶的纳米酶（LacNZs），所得到的铂锰和二氧化锰纳米花（NFs）表现出了类似漆酶的活性，其活性类似于来自瘤葡萄的漆酶。此外，这些 LacNZs 在广泛的 pH 值（3.0-7.5）、温度（4-70 °C）和离子强度（高达 500 mM NaCl）条件下显示出更强的稳定性。所开发的铂锰 NF/石墨电极类似于漆包线/石墨电极，可以高灵敏度（分别为 25 000 ± 12 A-M-1-m-2 和 1900 ± 9 A-M-1-m-2）检测 5-HIAA，线性范围分别为 0.3 - 15 μM 和 2 - 50 μM。传感器在低工作电位下工作，检测限分别为 0.16 和 1.4 μM，涵盖了尿液中 5-HIAA（1 - 50 μM）含量的正常和病理范围。它们已成功应用于各种疾病患者尿样中 5-HIAA 的检测，并显示出良好的回收率和重现性。此外，LacNZ 传感器的稳定性最好，可使用长达 20 天。

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来源期刊

Bioelectrochemistry 生物-电化学

CiteScore

9.10

自引率

6.00%

发文量

238

审稿时长

38 days

期刊介绍： An International Journal Devoted to Electrochemical Aspects of Biology and Biological Aspects of Electrochemistry Bioelectrochemistry is an international journal devoted to electrochemical principles in biology and biological aspects of electrochemistry. It publishes experimental and theoretical papers dealing with the electrochemical aspects of: • Electrified interfaces (electric double layers, adsorption, electron transfer, protein electrochemistry, basic principles of biosensors, biosensor interfaces and bio-nanosensor design and construction. • Electric and magnetic field effects (field-dependent processes, field interactions with molecules, intramolecular field effects, sensory systems for electric and magnetic fields, molecular and cellular mechanisms) • Bioenergetics and signal transduction (energy conversion, photosynthetic and visual membranes) • Biomembranes and model membranes (thermodynamics and mechanics, membrane transport, electroporation, fusion and insertion) • Electrochemical applications in medicine and biotechnology (drug delivery and gene transfer to cells and tissues, iontophoresis, skin electroporation, injury and repair). • Organization and use of arrays in-vitro and in-vivo, including as part of feedback control. • Electrochemical interrogation of biofilms as generated by microorganisms and tissue reaction associated with medical implants.