Effects of energy-gathered and energy-divergent ultrasound on structure, DNA extraction and adulterated quantification of sweet potato vermicelli and its starch.

IF 3.3 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Journal of the Science of Food and Agriculture Pub Date : 2024-10-31 DOI:10.1002/jsfa.13997

Diana Kavidia Muyembe, Miao Zhang, Hong-Nan Sun, Tai-Hua Mu

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Abstract

Background: The identification of adulterated sweet potato vermicelli faces significant challenges, seriously hindering the development of the vermicelli industry. Herein, we investigate effects of energy-gathered ultrasound (EGU) and energy-divergent ultrasound (EDU) (30, 40 and 50 W L^-1) on structure, DNA extraction and adulterated quantification of sweet potato vermicelli and its starch, thereby exploring their potential in adulteration of sweet potato vermicelli.

Results: EGU-assisted modified cetyltrimethylammonium bromide (CTAB) protocol with β-mercaptoethanol significantly improved DNA extraction from sweet potato vermicelli (223.7-249.2 ng μL^-1) and its starch (133.4-186.4 ng μL^-1), followed by EDU-assisted DNA extraction from sweet potato vermicelli (115.1-209.3 ng μL^-1) and its starch (33.4-61.0 ng μL^-1). Both EGU and EDU treatments resulted in the destruction of microstructure and crystalline structure, as well as changes in pasting and thermal properties of sweet potato vermicelli and its starch. Real-time polymerase chain reaction (PCR) amplification results revealed that EGU and EDU enhanced the efficiency of DNA amplification, and EDU showed smaller cycle threshold (C_t) values than EGU. In addition, EDU-assisted CTAB protocol combined with real-time PCR could detect levels of less than 1% of cassava and maize starches in sweet potato vermicelli.

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聚能超声波和发散超声波对红薯粉丝及其淀粉的结构、DNA 提取和掺假定量的影响。

背景：掺假红薯粉条的鉴别面临巨大挑战，严重阻碍了粉条产业的发展。在此，我们研究了能量聚集超声（EGU）和能量发散超声（EDU）（30、40 和 50 W L-1）对红薯粉丝及其淀粉的结构、DNA 提取和掺假定量的影响，从而探索它们在红薯粉丝掺假中的潜力：结果：EGU 辅助的改良十六烷基三甲基溴化铵（CTAB）方案与 β-巯基乙醇显著提高了红薯粉丝中 DNA 的提取率（223.7-249.2 ng μL-1）及其淀粉（133.4-186.4 ng μL-1）中的DNA提取率，其次是EDU辅助的红薯粉丝（115.1-209.3 ng μL-1）及其淀粉（33.4-61.0 ng μL-1）中的DNA提取率。EGU 和 EDU 处理均导致红薯粉条及其淀粉的微观结构和结晶结构遭到破坏，糊化性能和热性能也发生了变化。实时聚合酶链反应（PCR）扩增结果显示，EGU 和 EDU 提高了 DNA 的扩增效率，而且 EDU 的循环阈值（Ct）比 EGU 小。此外，EDU 辅助 CTAB 方案与实时 PCR 结合使用，可检测出红薯粉丝中低于 1%的木薯和玉米淀粉含量：结论：EDU 辅助 CTAB 方案与实时 PCR 相结合，有望成为一种灵敏可靠的粉条掺假分析工具。© 2024 化学工业协会。

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

期刊介绍： The Journal of the Science of Food and Agriculture publishes peer-reviewed original research, reviews, mini-reviews, perspectives and spotlights in these areas, with particular emphasis on interdisciplinary studies at the agriculture/ food interface. Published for SCI by John Wiley & Sons Ltd. SCI (Society of Chemical Industry) is a unique international forum where science meets business on independent, impartial ground. Anyone can join and current Members include consumers, business people, environmentalists, industrialists, farmers, and researchers. The Society offers a chance to share information between sectors as diverse as food and agriculture, pharmaceuticals, biotechnology, materials, chemicals, environmental science and safety. As well as organising educational events, SCI awards a number of prestigious honours and scholarships each year, publishes peer-reviewed journals, and provides Members with news from their sectors in the respected magazine, Chemistry & Industry . Originally established in London in 1881 and in New York in 1894, SCI is a registered charity with Members in over 70 countries.