Foliar application of carbon dots enhances nitrogen uptake and assimilation through CEPD1-dependent signaling in plants

IF 6.1 2区 生物学 Q1 PLANT SCIENCES Plant Physiology and Biochemistry Pub Date : 2024-10-24 DOI:10.1016/j.plaphy.2024.109229
Zhiyuan Pan , Huihui Zang , Yanjuan Li , Xiao Wang , Nan Xia , Chong Liu , Zongyun Li , Yonghua Han , Zhonghou Tang , Jian Sun
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Abstract

The use of nitrogen (N) fertilizers increases crop yield, but the accumulation of residual N in agricultural soils poses significant environmental risks. Improving the N use efficiency (NUE) of crops can help reduce N pollution. While nanomaterials have been shown to enhance crop agronomic traits, more research is needed to clarify the regulatory mechanisms involved. In this study, foliar spraying of carbon dots (CDs, 1 mg mL−1) derived from Salvia miltiorrhiza increased the activity of plasma membrane H+-ATPase in Arabidopsis thaliana roots, promoting the uptake, transport, and assimilation of NO3 and NH4+. The upregulation of N metabolism-related genes, such as AtAMTs and AtNRTs, was also observed in A. thaliana roots. Transcriptome analysis suggested that this regulatory effect is mediated by the shoot-to-root mobile polypeptide CEPD1 (C-terminally encoded peptide DOWNSTREAM 1) signaling pathway. Additionally, foliar application of CDs increased the NUE of sweetpotato (Ipomoea batatas (L.) Lam.) from 2.5% to 8.1%. The upregulation of genes such as CEPD1 in leaves was observed following CDs application under different N conditions. Finally, foliar spraying of CDs significantly increased field yield and enhanced tolerance to low N stress in sweetpotato. Overall, this study demonstrated that foliar application of CDs improved NUE in plants through CEPD1-dependent signaling.

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叶面喷施碳点可通过 CEPD1 依赖性信号增强植物对氮的吸收和同化。
使用氮(N)肥料可以提高作物产量,但农业土壤中残留氮的积累会带来巨大的环境风险。提高作物的氮利用效率(NUE)有助于减少氮污染。虽然纳米材料已被证明能提高作物的农艺性状,但还需要更多的研究来阐明其中的调节机制。在本研究中,叶面喷洒来自丹参的碳点(CDs,1 mg mL-1)可提高拟南芥根系质膜 H+-ATP 酶的活性,促进 NO3- 和 NH4+ 的吸收、转运和同化。在拟南芥根中还观察到氮代谢相关基因(如 AtAMTs 和 AtNRTs)的上调。转录组分析表明,这种调控效应是由芽到根的移动多肽 CEPD1(C-端编码肽 DOWNSTREAM 1)信号通路介导的。此外,叶面施用 CD 还能将甘薯(Ipomoea batatas (L.) Lam.)的净效率从 2.5% 提高到 8.1%。在不同的氮条件下,叶面喷施 CD 后,观察到 CEPD1 等基因上调。最后,叶面喷施 CD 显著提高了甘薯的田间产量,并增强了对低氮胁迫的耐受性。总之,本研究表明,叶面喷施 CD 可通过 CEPD1 依赖性信号转导改善植物的氮利用效率。
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来源期刊
Plant Physiology and Biochemistry
Plant Physiology and Biochemistry 生物-植物科学
CiteScore
11.10
自引率
3.10%
发文量
410
审稿时长
33 days
期刊介绍: Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.
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