Lab-on-a-chip models of cardiac inflammation.

IF 2.6 4区 工程技术 Q2 BIOCHEMICAL RESEARCH METHODS Biomicrofluidics Pub Date : 2024-10-29 eCollection Date: 2024-09-01 DOI:10.1063/5.0231735
Anna Maria Popovic, Matthew Ho Cheong Lei, Amid Shakeri, Ramak Khosravi, Milica Radisic
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Abstract

Cardiovascular diseases are the leading cause of morbidity and mortality worldwide with numerous inflammatory cell etiologies associated with impaired cardiac function and heart failure. Inflammatory cardiomyopathy, also known as myocarditis, is an acquired cardiomyopathy characterized by inflammatory cell infiltration into the myocardium with a high risk of progression to deteriorated cardiac function. Recently, amidst the ongoing COVID-19 pandemic, the emergence of acute myocarditis as a complication of SARS-CoV-2 has garnered significant concern. Given its mechanisms remain elusive in conjunction with the recent withdrawal of previously FDA-approved antiviral therapeutics and prophylactics due to unexpected cardiotoxicity, there is a pressing need for human-mimetic platforms to investigate disease pathogenesis, model dysfunctional features, and support pre-clinical drug screening. Traditional in vitro models for studying cardiovascular diseases have inherent limitations in recapitulating the complexity of the in vivo microenvironment. Heart-on-a-chip technologies, combining microfabrication, microfluidics, and tissue engineering techniques, have emerged as a promising approach for modeling inflammatory cardiac diseases like myocarditis. This review outlines the established and emerging conditions of inflamed myocardium, identifying key features essential for recapitulating inflamed myocardial structure and functions in heart-on-a-chip models, highlighting recent advancements, including the integration of anisotropic contractile geometry, cardiomyocyte maturity, electromechanical functions, vascularization, circulating immunity, and patient/sex specificity. Finally, we discuss the limitations and future perspectives necessary for the clinical translation of these advanced technologies.

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心脏炎症的芯片实验室模型
心血管疾病是全球发病率和死亡率的主要原因,许多炎症细胞病因都与心功能受损和心力衰竭有关。炎症性心肌病又称心肌炎,是一种获得性心肌病,其特点是炎性细胞浸润心肌,极易导致心功能恶化。最近,在 COVID-19 大流行期间,急性心肌炎作为 SARS-CoV-2 的一种并发症引起了人们的极大关注。鉴于其发病机制仍然难以捉摸,再加上最近美国食品及药物管理局批准的抗病毒疗法和预防药物因意外的心脏毒性而被撤消,因此迫切需要仿人类平台来研究疾病的发病机制、模拟功能障碍特征并支持临床前药物筛选。研究心血管疾病的传统体外模型在再现体内微环境的复杂性方面存在固有的局限性。芯片心脏技术结合了微加工、微流控和组织工程技术,已成为模拟心肌炎等炎症性心脏疾病的一种很有前景的方法。本综述概述了炎症心肌的既有条件和新兴条件,确定了在片上心脏模型中再现炎症心肌结构和功能的关键特征,重点介绍了最近的进展,包括各向异性收缩几何、心肌细胞成熟度、机电功能、血管化、循环免疫和患者/性别特异性的整合。最后,我们讨论了这些先进技术临床转化所需的局限性和未来展望。
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来源期刊
Biomicrofluidics
Biomicrofluidics 生物-纳米科技
CiteScore
5.80
自引率
3.10%
发文量
68
审稿时长
1.3 months
期刊介绍: Biomicrofluidics (BMF) is an online-only journal published by AIP Publishing to rapidly disseminate research in fundamental physicochemical mechanisms associated with microfluidic and nanofluidic phenomena. BMF also publishes research in unique microfluidic and nanofluidic techniques for diagnostic, medical, biological, pharmaceutical, environmental, and chemical applications. BMF offers quick publication, multimedia capability, and worldwide circulation among academic, national, and industrial laboratories. With a primary focus on high-quality original research articles, BMF also organizes special sections that help explain and define specific challenges unique to the interdisciplinary field of biomicrofluidics. Microfluidic and nanofluidic actuation (electrokinetics, acoustofluidics, optofluidics, capillary) Liquid Biopsy (microRNA profiling, circulating tumor cell isolation, exosome isolation, circulating tumor DNA quantification) Cell sorting, manipulation, and transfection (di/electrophoresis, magnetic beads, optical traps, electroporation) Molecular Separation and Concentration (isotachophoresis, concentration polarization, di/electrophoresis, magnetic beads, nanoparticles) Cell culture and analysis(single cell assays, stimuli response, stem cell transfection) Genomic and proteomic analysis (rapid gene sequencing, DNA/protein/carbohydrate arrays) Biosensors (immuno-assay, nucleic acid fluorescent assay, colorimetric assay, enzyme amplification, plasmonic and Raman nano-reporter, molecular beacon, FRET, aptamer, nanopore, optical fibers) Biophysical transport and characterization (DNA, single protein, ion channel and membrane dynamics, cell motility and communication mechanisms, electrophysiology, patch clamping). Etc...
期刊最新文献
Microfluidics for foodborne bacteria analysis: Moving toward multiple technologies integration. Wicking pumps for microfluidics. Lab-on-a-chip models of cardiac inflammation. In situ 3D polymerization (IS-3DP): Implementing an aqueous two-phase system for the formation of 3D objects inside a microfluidic channel. Non-invasive measurement of wall shear stress in microfluidic chip for osteoblast cell culture using improved depth estimation of defocus particle tracking method.
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