Impact of arbuscular mycorrhizal fungi on maize rhizosphere microbiome stability under moderate drought conditions

IF 6.1 1区 生物学 Q1 MICROBIOLOGY Microbiological research Pub Date : 2024-10-30 DOI:10.1016/j.micres.2024.127957
Yalin Chen , Chunyu Sun , Yuxin Yan , Dongxue Jiang , Shaoqi Huangfu , Lei Tian
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Abstract

With an alarming increase in global greenhouse gas emissions, unstable weather conditions are significantly impacting agricultural production. Drought stress is one of the frequent consequences of climate change that affects crop growth and yield. Addressing this issue is critical to ensure stable crop productivity under drought conditions. Arbuscular mycorrhizal fungi (AMF) establish symbiotic relationships with plants and enhance their resistance to adverse conditions. Effects of arbuscular mycorrhizal associations on the rhizosphere microbiome and root transcriptome under drought conditions have not been explored. Here, we investigated the effects of AMF and drought stress on rhizosphere microorganisms and root transcriptome of maize plants grown in chernozem soil. We used high-throughput sequencing data of bacterial 16S rRNA and fungal internal transcribed spacer regions (ITS) to identify rhizosphere microorganisms. Transcriptomic data were used to assess gene expression in maize plants under different treatments. Our results show that AMF maintains the composition of maize rhizosphere microorganisms under drought stress. In particular, the bacterial and fungal phyla maintained were Actinomycetes and Ascomycota, respectively. Transcriptomic data indicated that AMF influenced gene expression in maize plants under drought stress. Under drought stress, the expression of SWEET13, CHIT3, and RPL23A was significantly higher in the presence of AMF than it was without AMF inoculation, indicating better sugar transport, reduced malondialdehyde accumulation, and improved water use efficiency in AMF-inoculated maize plants. These findings suggest that AMF can enhance the resistance of maize to moderate drought stress by stabilising plant physical traits, which may help maintain the structure of the rhizosphere microbial community. This study provides valuable theoretical insights that should aid the utilization of AMF in sustainable agricultural practices.
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在中等干旱条件下,丛枝菌根真菌对玉米根瘤微生物组稳定性的影响
随着全球温室气体排放量的惊人增长,不稳定的天气条件对农业生产产生了重大影响。干旱胁迫是气候变化影响作物生长和产量的常见后果之一。解决这一问题对于确保干旱条件下作物的稳定产量至关重要。丛枝菌根真菌(AMF)与植物建立共生关系,增强植物对不利条件的抵抗力。干旱条件下,丛枝菌根结合对根瘤微生物组和根转录组的影响尚未得到探讨。在此,我们研究了AMF和干旱胁迫对生长在切尔诺泽姆土壤中的玉米根瘤微生物和根转录组的影响。我们利用细菌 16S rRNA 和真菌内部转录间隔区(ITS)的高通量测序数据来鉴定根圈微生物。转录组数据用于评估不同处理条件下玉米植株的基因表达。我们的研究结果表明,在干旱胁迫下,AMF能维持玉米根瘤微生物的组成。特别是,所维持的细菌和真菌门分别是放线菌门和子囊菌门。转录组数据表明,AMF 影响了干旱胁迫下玉米植株的基因表达。在干旱胁迫下,AMF存在时,SWEET13、CHIT3和RPL23A的表达量明显高于未接种AMF时,这表明接种AMF的玉米植株糖分转运能力更强,丙二醛积累减少,水分利用效率提高。这些研究结果表明,AMF 可以通过稳定植物的物理性状来增强玉米对中度干旱胁迫的抵抗力,这可能有助于维持根圈微生物群落的结构。这项研究提供了宝贵的理论见解,有助于在可持续农业实践中利用 AMF。
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来源期刊
Microbiological research
Microbiological research 生物-微生物学
CiteScore
10.90
自引率
6.00%
发文量
249
审稿时长
29 days
期刊介绍: Microbiological Research is devoted to publishing reports on prokaryotic and eukaryotic microorganisms such as yeasts, fungi, bacteria, archaea, and protozoa. Research on interactions between pathogenic microorganisms and their environment or hosts are also covered.
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