Candidate stem cell isolation and transplantation in Hexacorallia.

IF 7.5 1区生物学 Q1 CELL BIOLOGY Cell reports Pub Date : 2024-11-01 DOI:10.1016/j.celrep.2024.114944

Shani Talice, Itamar Kozlovski, Shany K Barkan, Grace A Snyder, Ton Sharoni, Tom Levy, Shelly Oisher, Aner Ottolenghi, Shir Eliachar, Ronit Ben-Romano, Keren Berlyne, Ronnie Yannai, Magda Lewandowska, Eliya Sultan, Oron Goldstein, Reuven Aharoni, Uzi Hadad, Claytus Davis, Yehu Moran, Orly Gershoni-Yahalom, Nikki Traylor-Knowles, Benyamin Rosental

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Abstract

Stem cells are the foundation for cell therapy due to their ability to self-renew, differentiate into other cell types, and persist throughout the life of an organism. Stem cell isolation and transplantation have not yet been established in Hexacorallia, a cnidarian subclass containing stony corals and sea anemones. Here, we demonstrate that candidate stem cells in the hexacorallian Nematostella vectensis can be transplanted into adult animals. These cells exhibited the hallmarks of stem cell functional properties; they integrated into recipients' tissues and rescued them from lethal doses of chemotherapy. Additionally, these cells proliferated and survived serial transplantations. Notably, we showed that this cellular subpopulation can be enriched by sorting using species-non-specific cell markers and that similar subpopulations of cells can be isolated from other hexacorallians, including stony corals. This research establishes the basis for studying stem cell biology on a functional level in Hexacorallia.

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候选干细胞的分离和移植。

干细胞能够自我更新、分化成其他细胞类型，并在生物体的整个生命过程中持续存在，因此是细胞疗法的基础。六孔目（一种包含石珊瑚和海葵的刺胞动物亚纲）的干细胞分离和移植尚未建立。在这里，我们证明了六孔动物Nematostella vectensis的候选干细胞可以移植到成年动物体内。这些细胞表现出干细胞功能特性的标志；它们融入受体组织，并从致命剂量的化疗中拯救它们。此外，这些细胞还能增殖并在连续移植中存活。值得注意的是，我们发现这种细胞亚群可以通过使用物种非特异性细胞标记物进行分选来富集，而且类似的细胞亚群也可以从其他六孔动物（包括石珊瑚）中分离出来。这项研究为从功能层面研究六孔珊瑚的干细胞生物学奠定了基础。

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来源期刊

Cell reports CELL BIOLOGY-

CiteScore

13.80

自引率

1.10%

发文量

1305

审稿时长

77 days

期刊介绍： Cell Reports publishes high-quality research across the life sciences and focuses on new biological insight as its primary criterion for publication. The journal offers three primary article types: Reports, which are shorter single-point articles, research articles, which are longer and provide deeper mechanistic insights, and resources, which highlight significant technical advances or major informational datasets that contribute to biological advances. Reviews covering recent literature in emerging and active fields are also accepted. The Cell Reports Portfolio includes gold open-access journals that cover life, medical, and physical sciences, and its mission is to make cutting-edge research and methodologies available to a wide readership. The journal's professional in-house editors work closely with authors, reviewers, and the scientific advisory board, which consists of current and future leaders in their respective fields. The advisory board guides the scope, content, and quality of the journal, but editorial decisions are independently made by the in-house scientific editors of Cell Reports.