A Ketogenic Diet Affects Gut Microbiota by Regulating Gut Microbiota and Promoting Hippocampal TRHR Expression to Combat Seizures

IF 2.8 4区 医学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Journal of Molecular Neuroscience Pub Date : 2024-11-03 DOI:10.1007/s12031-024-02245-z
Wenting Xiong, Xiaohui Lin, Xin Lin, Luyan Wu, Wanhui Lin
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Abstract

With the persistent challenge that epilepsy presents to therapeutic avenues, the study seeks to decipher the effects of the ketogenic diet (KD) on gut microbiota and subsequent epileptic outcomes. Mouse fecal samples from distinct KD and control diet (CD) cohorts underwent 16S rRNA sequencing. Differential genes of epileptic mice under these diets were sourced from the GEO database. The study melded in vivo and in vitro techniques to explore the nuanced interactions between KD, gut microbiota, and hippocampal TRHR dynamics. The KD regimen was found to result in a notable reduction in gut microbiota diversity when compared to the CD groups. Distinctive microbial strains, which are hypothesised to interact with epilepsy through G protein-coupled receptors, were spotlighted. In vivo, explorations affirmed that gut microbiota as central to KD’s anti-epileptic efficacy. Of 211 distinguished genes, the neuroactive ligand-receptor interaction pathway was underscored, particularly emphasizing TRHR and TRH. Clinical observations revealed a surge in hippocampal TRHR and TRH expressions influenced by KD, mirroring shifts in neuronal discharges. The KD, leveraging gut microbiota alterations, amplifies hippocampal TRHR expression. This finding provides a novel intervention strategy to reduce seizures.

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生酮饮食通过调节肠道微生物群和促进海马 TRHR 表达来影响肠道微生物群,从而对抗癫痫发作。
由于癫痫对治疗途径提出了持续性挑战,本研究试图破译生酮饮食(KD)对肠道微生物群和后续癫痫结果的影响。对来自不同生酮饮食(KD)和对照饮食(CD)队列的小鼠粪便样本进行了 16S rRNA 测序。癫痫小鼠在这些饮食中的差异基因来自 GEO 数据库。该研究将体内和体外技术相结合,探讨了KD、肠道微生物群和海马TRHR动态之间微妙的相互作用。研究发现,与CD组相比,KD方案导致肠道微生物群多样性明显减少。研究还发现了一些独特的微生物菌株,这些菌株被认为能通过G蛋白偶联受体与癫痫发生相互作用。体内研究证实,肠道微生物群是 KD 抗癫痫疗效的核心。在 211 个杰出基因中,神经活性配体-受体相互作用途径得到了强调,尤其是 TRHR 和 TRH。临床观察显示,海马 TRHR 和 TRH 的表达受 KD 影响而激增,反映了神经元放电的变化。KD利用肠道微生物群的改变,扩大了海马TRHR的表达。这一发现为减少癫痫发作提供了一种新的干预策略。
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来源期刊
Journal of Molecular Neuroscience
Journal of Molecular Neuroscience 医学-神经科学
CiteScore
6.60
自引率
3.20%
发文量
142
审稿时长
1 months
期刊介绍: The Journal of Molecular Neuroscience is committed to the rapid publication of original findings that increase our understanding of the molecular structure, function, and development of the nervous system. The criteria for acceptance of manuscripts will be scientific excellence, originality, and relevance to the field of molecular neuroscience. Manuscripts with clinical relevance are especially encouraged since the journal seeks to provide a means for accelerating the progression of basic research findings toward clinical utilization. All experiments described in the Journal of Molecular Neuroscience that involve the use of animal or human subjects must have been approved by the appropriate institutional review committee and conform to accepted ethical standards.
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