Yiheng Lim, Shumpei Kojima, Pradipta Mukherjee, Ibrahim Abd El-Sadek, Shuichi Makita, Yoshiaki Yasuno
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引用次数: 0
Abstract
The germination process of radish sprouts was investigated in detail using volumetric dynamic optical coherence tomography (OCT). Dynamic OCT involves the sequential acquisition of 16 OCT images and subsequent temporal variance analysis of each pixel, enabling non-invasive visualization of the cellular and tissue activities of plants. The radish sprouts were longitudinally investigated for up to 12 days, and changes in morphology and dynamic OCT image patterns were observed as the plants developed. The dynamic OCT signals in the vessels and growing roots were relatively high in the early stage of germination and decreased as the tissue matured. These results suggest that dynamic OCT is sensitive to water and nutrient transport as well as cellular activities associated with plant growth.
利用体积动态光学相干断层扫描（OCT）详细研究了萝卜芽的发芽过程。动态光学相干断层扫描包括连续采集 16 幅光学相干断层扫描图像，然后对每个像素进行时间方差分析，从而实现对植物细胞和组织活动的无创可视化。对萝卜芽进行了长达 12 天的纵向研究，观察了植物生长过程中形态和动态 OCT 图像模式的变化。发芽初期，血管和生长根中的动态 OCT 信号相对较高，随着组织的成熟，信号逐渐降低。这些结果表明，动态 OCT 对植物生长过程中的水分和养分运输以及细胞活动非常敏感。
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