Cryptosporidium parvum inactivation from short durations of treatment with ozonated water produced by an electrolytic generation system.

IF 1.8 3区 医学 Q2 PARASITOLOGY Parasitology Research Pub Date : 2024-11-06 DOI:10.1007/s00436-024-08390-z
Makoto Matsubayashi, Asako Haraguchi, Manami Morisaki, Hiromi Ikadai, Isao Teramoto, Yasutoshi Kido, Akira Kaneko
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Abstract

Cryptosporidium is a waterborne pathogen that causes diarrhea in vertebrates and humans (mainly C. hominis and C. parvum). Ozone (O3) is a powerful disinfectant due to its high oxidative characteristics, and it is used to inactivate microorganisms in drinking water. As an alternative to the gas dissolution system for producing ozone from oxygen, a simpler electrolytic ozone generation system has recently been developed. In the present study, the efficacy of the ozonated water produced by this system in inactivating Cryptosporidium parasites (C. parvum) was evaluated at different current intensities (which change the ozone concentrations) and short exposure times (15-60 s). Oocyst viability and integrity was assessed using vital dye staining, excystation assays, and scanning electron microscopy (SEM). SEM data revealed that oocyst walls were damaged by exposure to ozone molecules even at low concentrations (< 0.01 mg/l for 1 min) (current intensity 0.2 A), but that the excystation assay could not differentiate between deformed oocysts (dead) and partially excysted oocysts (alive). Exposure to ozonated water produced with a low current intensity (0.3 A) for 15 and 120 s resulted in the inactivation of 96.2% (CT value < 0.003) and 99.4% (CT value < 0.020) of the oocysts, respectively. Thus, it was estimated that a CT value more than 0.020 was required to inactivate > 99% of the C. parvum oocysts. These results suggested that the electrolytic ozone generation system may be more effective than gas dissolution ozone generation; however, further studies using additional approaches are needed to obtain clearer evidence.

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用电解生成系统产生的臭氧水进行短时间处理可灭活副隐孢子虫。
隐孢子虫是一种通过水传播的病原体,会导致脊椎动物和人类腹泻(主要是C. hominis和C. parvum)。臭氧(O3)具有高度氧化特性,是一种强力消毒剂,可用于灭活饮用水中的微生物。作为从氧气中产生臭氧的气体溶解系统的替代方案,最近开发出了一种更简单的电解臭氧发生系统。本研究评估了该系统产生的臭氧水在不同电流强度(改变臭氧浓度)和较短暴露时间(15-60 秒)下灭活隐孢子虫寄生虫(C. parvum)的功效。卵囊活力和完整性是通过活力染料染色法、卵囊外沉积测定法和扫描电子显微镜(SEM)进行评估的。扫描电子显微镜数据显示,即使臭氧浓度较低,卵囊壁也会受到破坏(99% 的副猪嗜血杆菌卵囊)。这些结果表明,电解臭氧发生系统可能比气体溶解臭氧发生系统更有效;不过,要获得更明确的证据,还需要使用其他方法进行进一步研究。
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来源期刊
Parasitology Research
Parasitology Research 医学-寄生虫学
CiteScore
4.10
自引率
5.00%
发文量
346
审稿时长
6 months
期刊介绍: The journal Parasitology Research covers the latest developments in parasitology across a variety of disciplines, including biology, medicine and veterinary medicine. Among many topics discussed are chemotherapy and control of parasitic disease, and the relationship of host and parasite. Other coverage includes: Protozoology, Helminthology, Entomology; Morphology (incl. Pathomorphology, Ultrastructure); Biochemistry, Physiology including Pathophysiology; Parasite-Host-Relationships including Immunology and Host Specificity; life history, ecology and epidemiology; and Diagnosis, Chemotherapy and Control of Parasitic Diseases.
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