Combining transcriptome and metabolome analyses to reveal the response of maize roots to Pb stress

IF 6.1 2区 生物学 Q1 PLANT SCIENCES Plant Physiology and Biochemistry Pub Date : 2024-11-02 DOI:10.1016/j.plaphy.2024.109265
Xiaoxiang Zhang , Bin Zhao , Xingye Ma , Xining Jin , Shilin Chen , Pingxi Wang , Guan Zhongrong , Xiangyuan Wu , Huaisheng Zhang
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Abstract

As a major food crop, maize (Zea mays L.) is facing a serious threat of lead (Pb) pollution. Research into its Pb tolerance is crucial for ensuring food security and human health, however, the molecular mechanism underlying the response to Pb remains incompletely understood. Here, we investigated the transcriptomic and metabolome of two maize lines (BY001, a Pb-resistant line; BY006, a Pb-sensitive line) under different concentrations of Pb stress (0, 500, 1000, 2000 and 3000 mg/L). The results showed that BY001 performed well, whereas the BY006 exhibited minimal development of lateral roots upon exposure to high concentration of Pb. The antioxidant enzyme activity of BY001 remained relatively stable, while that of BY006 declined significantly. Transcriptomic analysis revealed that under high concentration of Pb stress, BY001 produced 5057 differentially expressed genes, whereas BY006 produced 3374. Functional annotation showed that these genes were primarily involved in carbohydrate metabolism, root growth, and plant resistance to external Pb stress. Further untargeted metabolomics indicated that Pb stress triggered distinct alterations in the levels of 47 diverse metabolite types across 13 distinct classes, particularly amino acids, carbohydrates, and organic acids. A conjoint omics analysis suggested that the pathways of starch and sucrose metabolism, as well as cutin, suberin, and wax biosynthesis in BY001, play a key role in the Pb resistance. These findings elucidate the biological mechanisms employed by maize to counter the effects of Pb stress, and provide a basis for breeding of maize cultivars with low Pb accumulation or tolerance.

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结合转录组和代谢组分析揭示玉米根系对铅胁迫的响应
作为一种主要的粮食作物,玉米(Zea mays L.)正面临着铅(Pb)污染的严重威胁。研究玉米对铅的耐受性对确保粮食安全和人类健康至关重要,然而,人们对其对铅的反应的分子机制仍然不甚了解。在此,我们研究了两个玉米品系(抗铅品系 BY001 和铅敏感品系 BY006)在不同浓度的铅胁迫(0、500、1000、2000 和 3000 mg/L)下的转录组和代谢组。结果表明,在高浓度铅胁迫下,BY001 表现良好,而 BY006 的侧根发育极少。BY001 的抗氧化酶活性保持相对稳定,而 BY006 的抗氧化酶活性则显著下降。转录组分析表明,在高浓度铅胁迫下,BY001 产生了 5057 个差异表达基因,而 BY006 产生了 3374 个。功能注释表明,这些基因主要参与碳水化合物代谢、根系生长和植物对外部铅胁迫的抵抗。进一步的非靶向代谢组学研究表明,铅胁迫引发了 13 个不同类别的 47 种不同代谢物水平的明显变化,尤其是氨基酸、碳水化合物和有机酸。一项联合组学分析表明,BY001 中的淀粉和蔗糖代谢途径以及角质素、单宁和蜡的生物合成在抗铅性中起着关键作用。这些发现阐明了玉米抵御铅胁迫影响的生物机制,为培育低铅积累或耐受铅的玉米品种提供了依据。
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来源期刊
Plant Physiology and Biochemistry
Plant Physiology and Biochemistry 生物-植物科学
CiteScore
11.10
自引率
3.10%
发文量
410
审稿时长
33 days
期刊介绍: Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.
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