Infection of tomato plants by tomato yellow leaf curl virus (TYLCV) potentiates the ethylene and salicylic acid pathways to fend off root-knot nematode (Meloidogyne incognita) parasitism

IF 6.1 2区 生物学 Q1 PLANT SCIENCES Plant Physiology and Biochemistry Pub Date : 2024-11-04 DOI:10.1016/j.plaphy.2024.109271
{"title":"Infection of tomato plants by tomato yellow leaf curl virus (TYLCV) potentiates the ethylene and salicylic acid pathways to fend off root-knot nematode (Meloidogyne incognita) parasitism","authors":"","doi":"10.1016/j.plaphy.2024.109271","DOIUrl":null,"url":null,"abstract":"<div><div>In nature, it is common for plants to be infected by multiple pathogens simultaneously, and deciphering the underlying mechanisms of such interactions has remained elusive. The occurrence of root-knot nematode (RKN), <em>Meloidogyne incognita</em>, and tomato yellow leaf curl virus (TYLCV; <em>Begomovirus coheni</em>) has been reported in most tomato cultivation areas. We investigated the interaction between RKN and TYLCV in tomato plants at phenotypic, biochemical, and gene expression levels. Several treatments were considered including mock inoculation, inoculation with TYLCV or RKN alone, simultaneous inoculation with both TYLCV and RKN, and sequential inoculations with a five-day interval. Among them, simultaneous inoculation showed the highest impact on RKN suppression compared to mock-inoculated plants. Biochemical assays in the time-point experiments demonstrated that the pick of defense capacity of plants occurs at 48- and 72-h post-inoculation. Gene expression analyses utilizing marker genes from main hormonal pathways involved in plant defense, including salicylic acid (SA), jasmonic acid (JA), and ethylene (ET), indicated that ET and SA are highly involved in the potentiation of TYLCV-induced defense against RKN. To validate the action of SA and ET in the induction of defense against RKN by TYLCV, transgenic lines deficient in SA (<em>NahG</em>) and ET (<em>ACD</em>) accumulation were co-inoculated with TYLCV and RKN. Both transgenic lines failed to express TYLCV-induced defense against RKN. These findings demonstrate an antagonistic effect of TYLCV against RKN in tomato plants, mediated by SA and ET signaling pathways.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1000,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Physiology and Biochemistry","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0981942824009392","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0

Abstract

In nature, it is common for plants to be infected by multiple pathogens simultaneously, and deciphering the underlying mechanisms of such interactions has remained elusive. The occurrence of root-knot nematode (RKN), Meloidogyne incognita, and tomato yellow leaf curl virus (TYLCV; Begomovirus coheni) has been reported in most tomato cultivation areas. We investigated the interaction between RKN and TYLCV in tomato plants at phenotypic, biochemical, and gene expression levels. Several treatments were considered including mock inoculation, inoculation with TYLCV or RKN alone, simultaneous inoculation with both TYLCV and RKN, and sequential inoculations with a five-day interval. Among them, simultaneous inoculation showed the highest impact on RKN suppression compared to mock-inoculated plants. Biochemical assays in the time-point experiments demonstrated that the pick of defense capacity of plants occurs at 48- and 72-h post-inoculation. Gene expression analyses utilizing marker genes from main hormonal pathways involved in plant defense, including salicylic acid (SA), jasmonic acid (JA), and ethylene (ET), indicated that ET and SA are highly involved in the potentiation of TYLCV-induced defense against RKN. To validate the action of SA and ET in the induction of defense against RKN by TYLCV, transgenic lines deficient in SA (NahG) and ET (ACD) accumulation were co-inoculated with TYLCV and RKN. Both transgenic lines failed to express TYLCV-induced defense against RKN. These findings demonstrate an antagonistic effect of TYLCV against RKN in tomato plants, mediated by SA and ET signaling pathways.

Abstract Image

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
番茄黄叶卷曲病毒(TYLCV)感染番茄植株后会增强乙烯和水杨酸途径抵御根结线虫(Meloidogyne incognita)寄生的能力
在自然界中,植物同时受到多种病原体感染的情况很常见,而破译这种相互作用的内在机制却一直是个难题。根结线虫(RKN)、Meloidogyne incognita 和番茄黄卷叶病毒(TYLCV;Begomovirus coheni)在大多数番茄种植区都有发生。我们从表型、生化和基因表达水平研究了番茄植株中 RKN 和 TYLCV 之间的相互作用。我们考虑了几种处理方法,包括模拟接种、单独接种 TYLCV 或 RKN、同时接种 TYLCV 和 RKN 以及间隔五天接种。其中,与模拟接种的植株相比,同时接种对 RKN 的抑制作用最大。时间点实验中的生化分析表明,植物防御能力的提高发生在接种后的 48 小时和 72 小时。利用参与植物防御的主要激素途径(包括水杨酸(SA)、茉莉酸(JA)和乙烯(ET))的标记基因进行的基因表达分析表明,ET 和 SA 高度参与了 TYLCV 诱导的对 RKN 防御的增效作用。为了验证 SA 和 ET 在 TYLCV 诱导 RKN 防御过程中的作用,将 SA(NahG)和 ET(ACD)积累缺陷的转基因品系与 TYLCV 和 RKN 共同接种。这两个转基因品系都不能表达 TYLCV 诱导的对 RKN 的防御。这些研究结果表明,TYLCV 对番茄植株中的 RKN 具有拮抗作用,这种作用由 SA 和 ET 信号途径介导。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Plant Physiology and Biochemistry
Plant Physiology and Biochemistry 生物-植物科学
CiteScore
11.10
自引率
3.10%
发文量
410
审稿时长
33 days
期刊介绍: Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.
期刊最新文献
Riboflavin improves postharvest cold tolerance in zucchini fruit inducing non-enzymatic antioxidant response and phenolic metabolism Infection of tomato plants by tomato yellow leaf curl virus (TYLCV) potentiates the ethylene and salicylic acid pathways to fend off root-knot nematode (Meloidogyne incognita) parasitism Effects of water deficit on two cultivars of Hibiscus mutabilis: A comprehensive study on morphological, physiological, and metabolic responses Effect of green and UVA spectra, and pre-harvest treatments on biomass and metabolite yields of indoor cultivated stevia rebaudiana Combining transcriptome and metabolome analyses to reveal the response of maize roots to Pb stress
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1