Viability and diagnostic potential of tissues obtained through cryobiopsy

IF 2.4 Q2 RESPIRATORY SYSTEM Respiratory investigation Pub Date : 2024-11-01 DOI:10.1016/j.resinv.2024.10.011
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引用次数: 0

Abstract

Background

Transbronchial lung cryobiopsy is primarily used for diagnosing interstitial lung diseases and tumors, providing larger tissue samples with reduced tissue crushing than traditional biopsies. However, freezing during cryobiopsy may damage cells, potentially affecting diagnostic methods that require live cells, such as flow cytometry (FCM). We aimed to determine the extent of freezing-related cell damage in cryobiopsies using cells cultured in vitro.

Methods

To investigate the relationship between freezing duration and sample volume, Jurkat cells underwent freezing for durations ranging from 2 to 6 s, with 1-s intervals, using either 1-mm- or 1.7-mm cryoprobes. FCM was conducted to assess both cell viability (2, 4, and 6 s) and cell-surface molecule expression (3 and 6 s) over varying freezing times. Additionally, we describe a clinical case involving a 70-year-old man suspected of malignant lymphoma, in which tissue samples were obtained via both forceps biopsy and cryobiopsy methods to compare the pathological and cytological features between the methods.

Results

Harvested cell count increased with freezing duration, with a notable increase in viable cell percentage. Moreover, cells distant from the cryoprobe exhibited higher survival rates under milder freezing conditions. FCM revealed significantly higher marker expression levels in viable cryobiopsy samples than in non-viable samples. The clinical case demonstrated that cryobiopsy yields a significant proportion of live cells (>90%), with cytological findings consistent with those of non-frozen samples.

Conclusions

Cryobiopsy may be beneficial for histopathological diagnosis, providing sufficient viable cells for FCM, and can be used for diagnosing malignant lymphomas and other pulmonary conditions.
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通过冷冻活组织切片获取的组织的活力和诊断潜力
背景经支气管肺冷冻活检主要用于诊断肺间质疾病和肿瘤,与传统活检相比,冷冻活检能提供更大的组织样本,减少组织破碎。然而,冷冻活检过程中的冷冻可能会损伤细胞,从而可能影响需要活细胞的诊断方法,如流式细胞术(FCM)。为了研究冷冻持续时间与样本体积之间的关系,我们使用 1 毫米或 1.7 毫米的冷冻探针对 Jurkat 细胞进行了持续时间为 2 到 6 秒、间隔时间为 1 秒的冷冻。在不同的冷冻时间内,我们对细胞存活率(2、4 和 6 秒)和细胞表面分子表达(3 和 6 秒)进行了 FCM 评估。此外,我们还描述了一个 70 岁男性疑似恶性淋巴瘤的临床病例,病例中的组织样本是通过镊子活检和冷冻活检两种方法获得的,以比较两种方法的病理学和细胞学特征。此外,在较温和的冷冻条件下,远离冷冻探针的细胞存活率更高。FCM 显示,存活的冷冻活检样本的标记物表达水平明显高于未存活样本。临床病例表明,冷冻活组织切片可获得相当比例的活细胞(90%),细胞学结果与非冷冻样本一致。
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来源期刊
Respiratory investigation
Respiratory investigation RESPIRATORY SYSTEM-
CiteScore
4.90
自引率
6.50%
发文量
114
审稿时长
64 days
期刊最新文献
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