Mitigating environmental toxicity with hydrogen nanobubbles: A mitochondrial function-based approach to ecological restoration

IF 10.3 1区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES Environment International Pub Date : 2024-11-01 DOI:10.1016/j.envint.2024.109126

Han Bao , You Zhang , Shuang Lv , Shu Liu , Wenhong Fan

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Abstract

In biological systems, nanobubbles (NBs) effectively enhance hydrogen molecule retention and scavenging reactive oxygen species (ROS), but the underlying mechanisms remain elusive. To investigate this, we prepared hydrogen NB water samples with consistent dissolved hydrogen levels but varying NB densities to explore their physicochemical properties and effects on green algae (Chlorella vulgaris) under oxidative stress induced by copper ions (Cu²⁺) and cadmium ions (Cd²⁺). The results indicated a strong correlation between the hydrogen NB number density and the 25 % inhibitory concentration of Cu²⁺ over 24 h, with ROS removal efficiency increased with the NB number density. Gas chromatography showed that the hydrogen NBs in the solution had a high gas density that enhanced hydrogen transport into C. vulgaris. With regard to mitochondrial activity, hydrogen NBs were observed to enhance the function of mitochondrial complexes I and V and increase the mitochondrial membrane potential. Experiments with C. vulgaris mitochondrial electrodes showed that the electron transfer rates increased significantly in the presence of hydrogen NBs. We concluded that the high gas density of hydrogen NBs augments intracellular hydrogen delivery and strengthens mitochondrial functions.

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利用氢纳米气泡减轻环境毒性：基于线粒体功能的生态恢复方法

在生物系统中，纳米气泡（NBs）能有效增强氢分子的滞留能力并清除活性氧（ROS），但其基本机制仍然难以捉摸。为了研究这个问题，我们制备了溶解氢含量一致但纳米气泡密度不同的氢纳米气泡水样，以探索它们的理化性质以及在铜离子（Cu2+）和镉离子（Cd2+）诱导的氧化应激条件下对绿藻（Chlorella vulgaris）的影响。结果表明，在 24 小时内，氢 NB 数量密度与 25% 的 Cu2+ 抑制浓度之间存在很强的相关性，ROS 去除效率随 NB 数量密度的增加而提高。气相色谱法显示，溶液中的氢NB具有较高的气体密度，这增强了氢向粗毛蝇体内的运输。在线粒体活性方面，观察到氢 NB 增强了线粒体复合物 I 和 V 的功能，并提高了线粒体膜电位。用 C. vulgaris 线粒体电极进行的实验表明，在氢 NBs 的存在下，电子转移率显著增加。我们的结论是，氢气 NBs 的高气体密度增加了细胞内氢气的输送，并增强了线粒体的功能。

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来源期刊

Environment International 环境科学-环境科学

CiteScore

21.90

自引率

3.40%

发文量

734

审稿时长

2.8 months

期刊介绍： Environmental Health publishes manuscripts focusing on critical aspects of environmental and occupational medicine, including studies in toxicology and epidemiology, to illuminate the human health implications of exposure to environmental hazards. The journal adopts an open-access model and practices open peer review. It caters to scientists and practitioners across all environmental science domains, directly or indirectly impacting human health and well-being. With a commitment to enhancing the prevention of environmentally-related health risks, Environmental Health serves as a public health journal for the community and scientists engaged in matters of public health significance concerning the environment.