Stenotrophomonas pavanii MY01 induces phosphate precipitation of Cu(II) and Zn(II) by degrading glyphosate: performance, pathway and possible genes involved.

IF 4 2区 生物学 Q2 MICROBIOLOGY Frontiers in Microbiology Pub Date : 2024-10-23 eCollection Date: 2024-01-01 DOI:10.3389/fmicb.2024.1479902
Shengchen Zhao, Zitong Xu, Jihong Wang
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Abstract

Microbial bioremediation is an advanced technique for removing herbicides and heavy metals from agricultural soil. In this study, the strain Stenotrophomonas pavanii MY01 was used for its ability to degrade glyphosate, a phosphorus-containing organic compound, producing PO4 3- as a byproduct. PO4 3- is known to form stable precipitates with heavy metals, indicating that strain MY01 could potentially remove heavy metals by degrading glyphosate. Therefore, the present experiment induced phosphate precipitation from Cu(II) (Hereinafter referred to as Cu2+) and Zn(II) (Hereinafter referred to as Zn2+) by degrading glyphosate with strain MY01. Meanwhile, the whole genome of strain MY01 was mined for its glyphosate degradation mechanism and its heavy metal removal mechanism. The results of the study showed that the strain degraded glyphosate best at 34°C, pH = 7.7, and an inoculum of 0.7%, reaching 72.98% within 3d. The highest removal of Cu2+ and Zn2+ in the test was 75.95 and 68.54%, respectively. A comparison of strain MY01's genome with glyphosate degradation genes showed that protein sequences GE000474 and GE002603 had strong similarity to glyphosate oxidoreductase and C-P lyase. This suggests that these sequences may be key to the strain's ability to degrade glyphosate. The GE001435 sequence appears to be related to the phosphate pathway, which could enable phosphate excretion into the environment, where it forms stable coordination complexes with heavy metals.

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通过降解草甘膦诱导 Cu(II) 和 Zn(II) 磷酸盐沉淀的铺尾丝菌 MY01:性能、途径和可能的相关基因。
微生物生物修复是一种从农业土壤中去除除草剂和重金属的先进技术。在这项研究中,使用了 Stenotrophomonas pavanii MY01 菌株,因为它能够降解草甘膦(一种含磷有机化合物),产生副产品 PO4 3-。众所周知,PO4 3- 可与重金属形成稳定的沉淀物,这表明菌株 MY01 有可能通过降解草甘膦去除重金属。因此,本实验通过菌株 MY01 降解草甘膦,诱导 Cu(II)(以下简称 Cu2+)和 Zn(II)(以下简称 Zn2+)产生磷酸盐沉淀。同时,对菌株 MY01 的全基因组进行了草甘膦降解机理和重金属去除机理的挖掘。研究结果表明,该菌株在 34℃、pH=7.7、接种量为 0.7% 的条件下对草甘膦的降解效果最好,3d 内降解率达到 72.98%。试验中对 Cu2+ 和 Zn2+ 的去除率最高,分别为 75.95% 和 68.54%。将菌株 MY01 的基因组与草甘膦降解基因进行比较后发现,蛋白质序列 GE000474 和 GE002603 与草甘膦氧化还原酶和 C-P 裂解酶有很强的相似性。这表明这些序列可能是该菌株降解草甘膦能力的关键。GE001435 序列似乎与磷酸盐途径有关,可使磷酸盐排泄到环境中,并与重金属形成稳定的配位复合物。
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来源期刊
CiteScore
7.70
自引率
9.60%
发文量
4837
审稿时长
14 weeks
期刊介绍: Frontiers in Microbiology is a leading journal in its field, publishing rigorously peer-reviewed research across the entire spectrum of microbiology. Field Chief Editor Martin G. Klotz at Washington State University is supported by an outstanding Editorial Board of international researchers. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide.
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