Stenotrophomonas pavanii MY01 induces phosphate precipitation of Cu(II) and Zn(II) by degrading glyphosate: performance, pathway and possible genes involved.
{"title":"<i>Stenotrophomonas pavanii</i> MY01 induces phosphate precipitation of Cu(II) and Zn(II) by degrading glyphosate: performance, pathway and possible genes involved.","authors":"Shengchen Zhao, Zitong Xu, Jihong Wang","doi":"10.3389/fmicb.2024.1479902","DOIUrl":null,"url":null,"abstract":"<p><p>Microbial bioremediation is an advanced technique for removing herbicides and heavy metals from agricultural soil. In this study, the strain <i>Stenotrophomonas pavanii</i> MY01 was used for its ability to degrade glyphosate, a phosphorus-containing organic compound, producing PO<sub>4</sub> <sup>3-</sup> as a byproduct. PO<sub>4</sub> <sup>3-</sup> is known to form stable precipitates with heavy metals, indicating that strain MY01 could potentially remove heavy metals by degrading glyphosate. Therefore, the present experiment induced phosphate precipitation from Cu(II) (Hereinafter referred to as Cu<sup>2+</sup>) and Zn(II) (Hereinafter referred to as Zn<sup>2+</sup>) by degrading glyphosate with strain MY01. Meanwhile, the whole genome of strain MY01 was mined for its glyphosate degradation mechanism and its heavy metal removal mechanism. The results of the study showed that the strain degraded glyphosate best at 34°C, pH = 7.7, and an inoculum of 0.7%, reaching 72.98% within 3d. The highest removal of Cu<sup>2+</sup> and Zn<sup>2+</sup> in the test was 75.95 and 68.54%, respectively. A comparison of strain MY01's genome with glyphosate degradation genes showed that protein sequences GE000474 and GE002603 had strong similarity to glyphosate oxidoreductase and C-P lyase. This suggests that these sequences may be key to the strain's ability to degrade glyphosate. The GE001435 sequence appears to be related to the phosphate pathway, which could enable phosphate excretion into the environment, where it forms stable coordination complexes with heavy metals.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":null,"pages":null},"PeriodicalIF":4.0000,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11538021/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers in Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3389/fmicb.2024.1479902","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Microbial bioremediation is an advanced technique for removing herbicides and heavy metals from agricultural soil. In this study, the strain Stenotrophomonas pavanii MY01 was used for its ability to degrade glyphosate, a phosphorus-containing organic compound, producing PO43- as a byproduct. PO43- is known to form stable precipitates with heavy metals, indicating that strain MY01 could potentially remove heavy metals by degrading glyphosate. Therefore, the present experiment induced phosphate precipitation from Cu(II) (Hereinafter referred to as Cu2+) and Zn(II) (Hereinafter referred to as Zn2+) by degrading glyphosate with strain MY01. Meanwhile, the whole genome of strain MY01 was mined for its glyphosate degradation mechanism and its heavy metal removal mechanism. The results of the study showed that the strain degraded glyphosate best at 34°C, pH = 7.7, and an inoculum of 0.7%, reaching 72.98% within 3d. The highest removal of Cu2+ and Zn2+ in the test was 75.95 and 68.54%, respectively. A comparison of strain MY01's genome with glyphosate degradation genes showed that protein sequences GE000474 and GE002603 had strong similarity to glyphosate oxidoreductase and C-P lyase. This suggests that these sequences may be key to the strain's ability to degrade glyphosate. The GE001435 sequence appears to be related to the phosphate pathway, which could enable phosphate excretion into the environment, where it forms stable coordination complexes with heavy metals.
期刊介绍:
Frontiers in Microbiology is a leading journal in its field, publishing rigorously peer-reviewed research across the entire spectrum of microbiology. Field Chief Editor Martin G. Klotz at Washington State University is supported by an outstanding Editorial Board of international researchers. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide.