Effect of sodium selenite on the synthesis of glucosinolates and antioxidant capacity in Chinese cabbage (Brassica rapa L.ssp.pekinensis).

IF 3.4 3区 生物学 Q1 PLANT SCIENCES Physiology and Molecular Biology of Plants Pub Date : 2024-10-01 Epub Date: 2024-10-03 DOI:10.1007/s12298-024-01513-x
Yafang Luo, Shuang Zhao, Huan Wang, Huixia Bai, Qi Hu, Linlin Zhao, Tianyi Ma, Zhenyu Fan, Yushu Wang
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Abstract

Chinese cabbage (Brassica rapa ssp. pekinensis) is a globally cultivated and consumed leafy vegetable due to its abundant plant secondary metabolites and antioxidant compounds, including flavonoids, ascorbic acids, glucosinolates, and vitamins, which have been reported to confer health-promoting effects. Glucosinolates components in leaves of Chinese cabbage plantlets under different concentrations of sodium selenite (0, 30, and 50 μmol/L) were analyzed. Seven glucosinolates were identified and quantified using UHPLC-QTOF-MS. Finally, treatments with 30 and 50 μmol/L Na2SeO3 solution significantly increased the levels of total selenium content as well as total phenols, flavonoids, anthocyanins, and DPPH free radical scavenging ability in Chinese cabbage seedlings. Our results revealed that 30 μmol/L Na2SeO3 effectively enhanced aliphatic glucosinolate levels and total glucosinolate content while causing a significant reduction in indole glucosinolates. Furthermore, downregulation was observed for BrCYP79F1, BrBCAT4, and BrMAM1 genes associated with aliphatic glucosinolate synthesis. Conversely, BrMYB28 and BrCYP83A1 genes exhibited significant upregulation. Thus, the positive influence of Na2SeO3 on glucosinolate biosynthesis in Chinese cabbage can be attributed to the upregulation of key genes related to this process.

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亚硒酸钠对大白菜(Brassica rapa L.ssp.pekinensis)葡萄糖苷酸盐合成和抗氧化能力的影响
大白菜(Brassica rapa ssp. pekinensis)是一种全球栽培和食用的叶菜,因其含有丰富的植物次生代谢产物和抗氧化化合物,包括类黄酮、抗坏血酸、葡萄糖苷酸盐和维生素,据报道具有促进健康的作用。本研究分析了不同浓度亚硒酸钠(0、30 和 50 μmol/L)条件下小白菜叶片中的葡萄糖苷酸盐成分。使用 UHPLC-QTOF-MS 对七种葡萄糖苷酸进行了鉴定和定量。最后,用 30 和 50 μmol/L Na2SeO3 溶液处理后,大白菜幼苗的总硒含量、总酚、类黄酮、花青素和 DPPH 自由基清除能力都有显著提高。结果表明,30 μmol/L Na2SeO3 能有效提高脂肪族葡萄糖苷酸含量和总葡萄糖苷酸含量,同时显著降低吲哚葡萄糖苷酸含量。此外,还观察到与脂肪族葡萄糖苷酸合成有关的 BrCYP79F1、BrBCAT4 和 BrMAM1 基因下调。相反,BrMYB28 和 BrCYP83A1 基因则表现出明显的上调。因此,Na2SeO3 对大白菜中葡萄糖苷酸生物合成的积极影响可归因于与该过程相关的关键基因的上调。
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来源期刊
CiteScore
7.10
自引率
0.00%
发文量
126
期刊介绍: Founded in 1995, Physiology and Molecular Biology of Plants (PMBP) is a peer reviewed monthly journal co-published by Springer Nature. It contains research and review articles, short communications, commentaries, book reviews etc., in all areas of functional plant biology including, but not limited to plant physiology, biochemistry, molecular genetics, molecular pathology, biophysics, cell and molecular biology, genetics, genomics and bioinformatics. Its integrated and interdisciplinary approach reflects the global growth trajectories in functional plant biology, attracting authors/editors/reviewers from over 98 countries.
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