Artificial miRNAs and target-mimics as potential tools for crop improvement.

Abstract

MicroRNAs (miRNAs) are endogenous, small molecules that negatively regulate gene expression to control the normal development and stress response in plants. They mediate epigenetic changes and regulate gene expression at both transcriptional and post-transcriptional levels. Synthetic biology approaches have been utilized to design efficient artificial miRNAs (amiRNAs) or target-mimics to regulate specific gene expression for understanding the biological function of genes and crop improvement. The amiRNA based gene silencing is an effective technique to "turn off" gene expression, while miRNA target-mimics or decoys are used for efficiently down regulating miRNAs and "turn on" gene expression. In this context, the development of endogenous target-mimics (eTMs) and short tandem target mimics (STTMs) represent promising biotechnological tools for enhancing crop traits like stress tolerance and disease resistance. Through this review, we present the recent developments in understanding plant miRNA biogenesis, which is utilized for the efficient design and development of amiRNAs. This is important to incorporate the artificially synthesized miRNAs as internal components and utilizing miRNA biogenesis pathways for the programming of synthetic circuits to improve crop tolerance to various abiotic and biotic stress factors. The review also examines the recent developments in the use of miRNA target-mimics or decoys for efficiently down regulating miRNAs for trait improvement. A perspective analysis and challenges on the use of amiRNAs and STTM as potent tools to engineer useful traits in plants have also been presented.