Comparative transcriptome analysis highlights resistance regulatory networks of maize in response to Exserohilum turcicum infection at the early stage.

IF 5.4 2区生物学 Q1 PLANT SCIENCES Physiologia plantarum Pub Date : 2024-11-01 DOI:10.1111/ppl.14615

MingRui Li, Xin Qi, Dan Li, Zhiqiang Wu, Meiyi Liu, Weiguang Yang, Zhenyuan Zang, Liangyu Jiang

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Abstract

Northern corn leaf blight, caused by Exserohilum turcicum (E. turcicum), is one of the most destructive diseases in maize, leading to serious yield losses. However, the underlying molecular mechanisms of E. turcicum infection response in maize remain unclear. In this study, we performed comparative transcriptome analysis in resistant maize inbred line J9D207 (R) and susceptible maize inbred line PH4CV (S) after infecting with E. turcicum at 0 h, 24 h and 72 h, respectively. Compared with 0 h, 9656 (24 h) and 8748 (72 h) differentially expressed genes (DEGs) were identified in J9D207, and 7915 (24 h) and 7865 (72 h) DEGs were identified in PH4CV. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that alpha-linolenic acid metabolism, benzoxazinoid biosynthesis, flavonoid biosynthesis and phenylpropanoid biosynthesis might be involved in maize defense reactions. Some DEGs coded for transcription factors, such as MYB-related, ERF, NAC, bZIP, bHLH and WRKY families, which indicated that they may participate in resistance against E. turcicum. In addition, DEGs involved in SA, JA, ABA and ET signaling pathways were revealed. Moreover, 75 SOD activity-related genes and 421 POD activity-related genes were identified through weighted gene co-expression network analysis (WGCNA), respectively. These results provide a novel insight into the resistance mechanism of maize in response to E. turcicum inoculation.

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比较转录组分析凸显了玉米在早期阶段对 Exserohilum turcicum 感染的抗性调控网络。

由 Exserohilum turcicum（E. turcicum）引起的北玉米叶枯病是玉米中最具破坏性的病害之一，会导致严重的产量损失。然而，E. turcicum 感染玉米的潜在分子机制仍不清楚。本研究对抗性玉米近交系 J9D207（R）和易感玉米近交系 PH4CV（S）分别在 0 h、24 h 和 72 h 感染 E. turcicum 后的转录组进行了比较分析。与 0 h 相比，J9D207 发现了 9656 个差异表达基因（24 h）和 8748 个差异表达基因（72 h），PH4CV 发现了 7915 个差异表达基因（24 h）和 7865 个差异表达基因（72 h）。京都基因组百科全书》（KEGG）富集分析表明，α-亚麻酸代谢、苯并恶嗪类生物合成、黄酮类生物合成和苯丙类生物合成可能参与了玉米的防御反应。一些 DEGs 为转录因子编码，如 MYB 相关、ERF、NAC、bZIP、bHLH 和 WRKY 家族，这表明它们可能参与了对 E. turcicum 的抗性。此外，还发现了参与 SA、JA、ABA 和 ET 信号通路的 DEGs。此外，通过加权基因共表达网络分析（WGCNA），还分别发现了 75 个 SOD 活性相关基因和 421 个 POD 活性相关基因。这些结果为研究玉米对E. turcicum接种的抗性机制提供了新的视角。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Physiologia plantarum 生物-植物科学

CiteScore

11.00

自引率

3.10%

发文量

224

审稿时长

3.9 months

期刊介绍： Physiologia Plantarum is an international journal committed to publishing the best full-length original research papers that advance our understanding of primary mechanisms of plant development, growth and productivity as well as plant interactions with the biotic and abiotic environment. All organisational levels of experimental plant biology – from molecular and cell biology, biochemistry and biophysics to ecophysiology and global change biology – fall within the scope of the journal. The content is distributed between 5 main subject areas supervised by Subject Editors specialised in the respective domain: (1) biochemistry and metabolism, (2) ecophysiology, stress and adaptation, (3) uptake, transport and assimilation, (4) development, growth and differentiation, (5) photobiology and photosynthesis.