Nickel phytoremediation potential of Plantago major L.: Transcriptome analysis

IF 4.5 2区 生物学 Q2 ENVIRONMENTAL SCIENCES Environmental and Experimental Botany Pub Date : 2024-10-31 DOI:10.1016/j.envexpbot.2024.106020
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Abstract

Plantago major L, a ubiquitous perennial weed thriving in diverse harsh environments, possesses a substantial reservoir of resistance genes, particularly those conferring resistance to heavy metals. Despite its prevalence, the intricate molecular mechanisms underpinning its exceptional ability to endure heavy metal pollution remain largely unexplored. Through transcriptome analysis, this study intended to reveal the mechanisms behind the enrichment of heavy metal Ni (nickel) in P. major and its potential for phytoremediation. In total, 7848 differentially expressed genes (DEGs) exhibited dynamic changes in tissues treated with different Ni concentrations. It was discovered that the root of P. major exhibited a more pronounced and significant response when exposed to higher concentrations of Ni. Furthermore, the upregulated genes associated with adversity stress were significantly observed in response to Ni stress. The majority of pathways related to plant growth and photosynthesis were significantly reduced; however, pathways related to metabolite synthesis, chitin synthesis, and adversity signal transduction were stimulated, and pathways related to root cell wall organization or biogenesis were suppressed. We identified that the rate-limiting enzyme PmHISN1A/B in the histidine synthesis pathway significantly enhanced Ni tolerance of the transgenic Arabidopsis without side effects, which was different to its Arabidopsis homologs. The study uncovered a molecular basis for the Ni tolerance of P. major, a heavy metal remediation plant, and provided potential genetic resources to cultivate novel P. major varieties or breed stress resilience crops.
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大车前子的镍植物修复潜力:转录组分析
大车前草(Plantago major L)是一种在各种恶劣环境中生长的多年生杂草,它拥有大量的抗性基因,尤其是那些赋予重金属抗性的基因。尽管大车前草普遍存在,但其耐受重金属污染的特殊能力的复杂分子机制在很大程度上仍未得到探索。本研究旨在通过转录组分析,揭示重金属 Ni(镍)在大鳞栉孔雀草中的富集机制及其植物修复潜力。共有 7848 个差异表达基因(DEGs)在经不同浓度镍处理的组织中表现出动态变化。研究发现,当暴露于较高浓度的镍时,大戟科植物的根部表现出更明显、更显著的反应。此外,与逆境胁迫相关的上调基因在对镍胁迫的反应中也有显著表现。大多数与植物生长和光合作用相关的通路显著减少;然而,与代谢物合成、几丁质合成和逆境信号转导相关的通路受到刺激,与根细胞壁组织或生物生成相关的通路受到抑制。我们发现组氨酸合成途径中的限速酶PmHISN1A/B能显著增强转基因拟南芥对镍的耐受性,且无副作用,这与其拟南芥同源物不同。该研究揭示了拟南芥这种重金属修复植物耐镍的分子基础,为培育拟南芥新品种或培育抗逆作物提供了潜在的遗传资源。
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来源期刊
Environmental and Experimental Botany
Environmental and Experimental Botany 环境科学-环境科学
CiteScore
9.30
自引率
5.30%
发文量
342
审稿时长
26 days
期刊介绍: Environmental and Experimental Botany (EEB) publishes research papers on the physical, chemical, biological, molecular mechanisms and processes involved in the responses of plants to their environment. In addition to research papers, the journal includes review articles. Submission is in agreement with the Editors-in-Chief. The Journal also publishes special issues which are built by invited guest editors and are related to the main themes of EEB. The areas covered by the Journal include: (1) Responses of plants to heavy metals and pollutants (2) Plant/water interactions (salinity, drought, flooding) (3) Responses of plants to radiations ranging from UV-B to infrared (4) Plant/atmosphere relations (ozone, CO2 , temperature) (5) Global change impacts on plant ecophysiology (6) Biotic interactions involving environmental factors.
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