Enhancement of ketocarotenoid production via heterologous expression of orange protein from Ipomoea batatas in indigenous microalga Ettlia sp.

IF 4.6 2区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Algal Research-Biomass Biofuels and Bioproducts Pub Date : 2024-10-21 DOI:10.1016/j.algal.2024.103767
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Abstract

Astaxanthin, one of the most powerful natural antioxidants, is used in high-value industries such as those of cosmetics, nutraceuticals, and food products derived from microalgae. In this study, Ettlia sp. mutants were generated by expressing two types of heterologous orange proteins, IbOr and IbOr-R96H, in which the 96th arginine of IbOr was substituted with histidine derived from Ipomoea batatas to enhance astaxanthin production. The Ett-IbOr-R96H mutant showed a 2.4-fold increase in β-carotene content compared to the wildtype (4.59 mg g−1DCW), reaching 10.82 mg g−1 under high-light conditions via two-phase cultivation. Under the stress treatment combination of high-light intensity and nitrogen deprivation, total carotenoid content increased to 17.24 mg L−1 and 21.94 mg L−1 in Ett-IbOr and Ett-IbOr-R96H, respectively. The astaxanthin and canthaxanthin contents in Ett-IbOr-R96H was 4.89 mg L−1 and 0.47 mg L−1, respectively, which were 1.8- and 1.5-fold higher, respectively, than those in Ett-IbOr. Additionally, photosynthetic efficiency (Fv/Fm) recovered in Ett-IbOr-R96H under dual-stress conditions compared to the wildtype while reactive oxygen species levels decreased throughout the cultivation period. Our findings suggest that the heterologous IbOr expression in Ettlia sp. may be an effective approach for enhancing the production of ketocarotenoids and improving stress resistance for industrial applications.

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通过在本地微藻 Ettlia sp. 中异源表达来自 Ipomoea batatas 的橙色蛋白提高酮类胡萝卜素的产量
虾青素是最强大的天然抗氧化剂之一,被用于化妆品、营养保健品和微藻食品等高价值产业。本研究通过表达两种异源橙蛋白(IbOr 和 IbOr-R96H)生成了 Ettlia sp.突变体,其中 IbOr 的第 96 个精氨酸被来自 Ipomoea batatas 的组氨酸取代,以提高虾青素的产量。与野生型(4.59 mg g-1DCW)相比,Ett-IbOr-R96H突变体的β-胡萝卜素含量增加了2.4倍,在高光条件下通过两相培养达到10.82 mg g-1。在高光照强度和氮素剥夺的胁迫处理组合下,Ett-IbOr和Ett-IbOr-R96H的类胡萝卜素总含量分别增加到17.24毫克/升和21.94毫克/升。Ett-IbOr-R96H 的虾青素和角黄素含量分别为 4.89 mg L-1 和 0.47 mg L-1,分别是 Ett-IbOr 的 1.8 倍和 1.5 倍。此外,与野生型相比,Ett-IbOr-R96H 在双重胁迫条件下的光合效率(Fv/Fm)有所恢复,而活性氧水平在整个培养期间均有所下降。我们的研究结果表明,在 Ettlia sp.中异源表达 IbOr 可能是提高酮类胡萝卜素产量和抗逆性的有效方法,可用于工业应用。
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来源期刊
Algal Research-Biomass Biofuels and Bioproducts
Algal Research-Biomass Biofuels and Bioproducts BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
CiteScore
9.40
自引率
7.80%
发文量
332
期刊介绍: Algal Research is an international phycology journal covering all areas of emerging technologies in algae biology, biomass production, cultivation, harvesting, extraction, bioproducts, biorefinery, engineering, and econometrics. Algae is defined to include cyanobacteria, microalgae, and protists and symbionts of interest in biotechnology. The journal publishes original research and reviews for the following scope: algal biology, including but not exclusive to: phylogeny, biodiversity, molecular traits, metabolic regulation, and genetic engineering, algal cultivation, e.g. phototrophic systems, heterotrophic systems, and mixotrophic systems, algal harvesting and extraction systems, biotechnology to convert algal biomass and components into biofuels and bioproducts, e.g., nutraceuticals, pharmaceuticals, animal feed, plastics, etc. algal products and their economic assessment
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