Visual detection of uric acid in serum through catalytic oxidation by a novel cellulose membrane biosensor with schiff base immobilized uricase

IF 10.7 1区生物学 Q1 BIOPHYSICS Biosensors and Bioelectronics Pub Date : 2024-11-09 DOI:10.1016/j.bios.2024.116912

Qi Sun , Shiji Miao , Wenlong Yu , En-Yu Jiang , Mixue Gong , Genyan Liu , Xiaogang Luo , Ming-Zhi Zhang

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Abstract

Uric acid (UA) serves as an important biochemical marker of various diseases, making the development of a novel method for its rapid and straightforward visual detection highly valuable. In this study, a uricase-based cellulose membrane biosensor (UCMB) was constructed by immobilizing uricase via a Schiff base reaction and nitroblue tetrazolium chloride (NBT) through adsorption. The UCMB detects UA through a mechanism in which uricase catalyzes the oxidation of UA, generation O₂^−· radicals that subsequently oxidize NBT to formazan, producing a distinctive color change from yellow to purple. The UCMB demonstrated successful visual detection of UA within 15 min, allowing for rapid naked-eye analysis. Additionally, the biosensor quantitatively detected UA over a broad linear range from 0 to 1000 μM, with a low detection limit of 3.88 μM. Most notably, the UCMB has accurately measured UA in human serum samples, comparable to the results from a commercial UA meter. These findings suggest that the UCMB can serve as a simple and reliable tool for early diagnosis of UA-related diseases.

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通过新型纤维素膜生物传感器的催化氧化作用，以肉眼可见的方式检测血清中的尿酸。

尿酸（UA）是多种疾病的重要生化标志物，因此开发一种快速、直接的肉眼检测尿酸的新方法具有重要价值。本研究通过希夫碱反应固定尿酸酶，并通过吸附作用固定硝基蓝氯化四氮唑（NBT），构建了基于尿酸酶的纤维素膜生物传感器（UCMB）。UCMB 检测尿酸的机制是尿酸酶催化尿酸氧化，生成 O2 自由基，然后将 NBT 氧化成甲酰肼，产生从黄色到紫色的独特颜色变化。UCMB 在 15 分钟内就成功地检测出了尿酸，实现了快速肉眼分析。此外，该生物传感器可在 0 至 1000 μM 的宽线性范围内定量检测尿素氮，检测限低至 3.88 μM。最值得注意的是，UCMB 能准确测量人体血清样本中的尿素氮，与商用尿素氮检测仪的结果相当。这些研究结果表明，UCMB 可以作为一种简单可靠的工具，用于早期诊断尿酸相关疾病。

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来源期刊

Biosensors and Bioelectronics 工程技术-电化学

CiteScore

20.80

自引率

7.10%

发文量

1006

审稿时长

29 days

期刊介绍： Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.