Impact of hexafluoropropylene oxide trimer acid (HFPO-TA) on sex differentiation after exposures during different development stages of zebrafish (Danio rerio)

IF 3.9 3区医学 Q2 FOOD SCIENCE & TECHNOLOGY Food and Chemical Toxicology Pub Date : 2024-11-12 DOI:10.1016/j.fct.2024.115108

Shasha Dong , Jianhui Xu , Xianghan Meng, Xiangyue Jiang, Dan Yang, Xiaohui Zhao, Xiaoying Li, Guanghui Ding

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Abstract

Hexafluoropropylene oxide trimer acid (HFPO-TA), a novel alternative to perfluorooctanoic acid (PFOA), has been widely used and ubiquitously detected in aquatic environments. However, its potential effects on sex differentiation of aquatic organisms are not well known. Therefore, in this study, zebrafish were exposed to HFPO-TA at different development stages (0–21, 21–42, and 42–63 dpf) to investigate the effects on sex differentiation and its underlying mechanisms. All three exposures to HFPO-TA resulted in the feminization of zebrafish, and the impact of Stage II was most significant. The transcription levels of key genes related to female differentiation (bpm15, cyp19a1a, esr1, vtg1, and sox9b) were up-regulated, while those of key genes related to male differentiation (dmrt1, gata4, amh, and sox9a) were down-regulated, which could lead to the feminization. In addition, it was found that the dysregulations of these genes were prolonged in adult zebrafish even through a long recovery, which could cause sex imbalance in populations. Therefore, HFPO-TA might not be a safe alternative to PFOA, and more evidences from multi- and transgenerational toxicology are warranted.

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斑马鱼（Danio rerio）不同发育阶段接触六氟环氧丙烷三聚酸（HFPO-TA）后对性别分化的影响。

六氟环氧丙烷三聚酸（HFPO-TA）是全氟辛酸（PFOA）的一种新型替代品，已被广泛使用并在水生环境中普遍检测到。然而，人们对其对水生生物性别分化的潜在影响还不甚了解。因此，在本研究中，斑马鱼在不同的发育阶段（0-21、21-42 和 42-63 dpf）暴露于 HFPO-TA，以研究其对性别分化的影响及其内在机制。暴露于 HFPO-TA 的三个阶段都会导致斑马鱼雌性化，其中第二阶段的影响最为显著。与雌性分化相关的关键基因（bpm15、cyp19a1a、esr1、vtg1和sox9b）的转录水平被上调，而与雄性分化相关的关键基因（dmrt1、gata4、amh和sox9a）的转录水平被下调，这可能会导致雌性化。此外，研究还发现，这些基因的失调在成年斑马鱼体内即使经过长期恢复也会持续，这可能会导致种群性别失衡。因此，HFPO-TA 可能不是全氟辛烷磺酸的安全替代品，还需要更多的多代和跨代毒理学证据。

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来源期刊

Food and Chemical Toxicology 工程技术-毒理学

CiteScore

10.90

自引率

4.70%

发文量

651

审稿时长

31 days

期刊介绍： Food and Chemical Toxicology (FCT), an internationally renowned journal, that publishes original research articles and reviews on toxic effects, in animals and humans, of natural or synthetic chemicals occurring in the human environment with particular emphasis on food, drugs, and chemicals, including agricultural and industrial safety, and consumer product safety. Areas such as safety evaluation of novel foods and ingredients, biotechnologically-derived products, and nanomaterials are included in the scope of the journal. FCT also encourages submission of papers on inter-relationships between nutrition and toxicology and on in vitro techniques, particularly those fostering the 3 Rs. The principal aim of the journal is to publish high impact, scholarly work and to serve as a multidisciplinary forum for research in toxicology. Papers submitted will be judged on the basis of scientific originality and contribution to the field, quality and subject matter. Studies should address at least one of the following: -Adverse physiological/biochemical, or pathological changes induced by specific defined substances -New techniques for assessing potential toxicity, including molecular biology -Mechanisms underlying toxic phenomena -Toxicological examinations of specific chemicals or consumer products, both those showing adverse effects and those demonstrating safety, that meet current standards of scientific acceptability. Authors must clearly and briefly identify what novel toxic effect (s) or toxic mechanism (s) of the chemical are being reported and what their significance is in the abstract. Furthermore, sufficient doses should be included in order to provide information on NOAEL/LOAEL values.