Identification of hub genes in the crosstalk between type 2 diabetic nephropathy and obesity according to bioinformatics analysis.

IF 3.5 4区生物学 Q2 ENDOCRINOLOGY & METABOLISM Adipocyte Pub Date : 2024-12-01 Epub Date: 2024-11-11 DOI:10.1080/21623945.2024.2423723

Shaomin Shi, Ke Ding, Feng Chen, Mei Yang, Lihua Ni, Xiaoyan Wu

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Abstract

Diabetic nephropathy (DN) and obesity bring a huge burden to society. Obesity plays a crucial role in the progression of type 2 DN, but the pathophysiology remains unclear. Thus, we aimed the explore the association between type 2 DN and obesity using bioinformatics method. The gene expression profiles of type 2 DN (GSE96804) and obesity (GSE94752) were downloaded from the Gene Expression Omnibus (GEO) database. The differentially expressed genes (DEGs) were screened with the thresholds defined as |log2FC| ≥1 and P<0.05. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed. Subsequently, a protein-protein interaction network was constructed based on the STRING database. Hub genes were identified, and the co-expression network was constructed. Finally, the hub genes were verified in clinical samples of 24 patients by immunohistochemistry. A total of 17 common DEGs were identified. Finally, two overlapping hub genes were identified (CCL18, C1QC). C1QC has been verified in clinical specimens. Using bioinformatics methods, the present study analyzed the common DEGs and the potential pathogenic mechanisms involved in type 2 DN and obesity. C1QC was the hub gene. Further studies are needed to clarify the specific relationships among C1QC, type 2 DN and obesity.

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根据生物信息学分析确定 2 型糖尿病肾病与肥胖之间相互影响的枢纽基因。

糖尿病肾病（DN）和肥胖症给社会带来了巨大负担。肥胖在 2 型糖尿病肾病的发展过程中起着至关重要的作用，但其病理生理学仍不清楚。因此，我们旨在利用生物信息学方法探讨 2 型糖尿病肾病与肥胖之间的关联。我们从基因表达总库（GEO）数据库下载了 2 型 DN（GSE96804）和肥胖（GSE94752）的基因表达谱。筛选差异表达基因（DEGs）的阈值定义为 |log2FC| ≥1和 P

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来源期刊

Adipocyte Medicine-Histology

CiteScore

6.50

自引率

3.00%

发文量

审稿时长

32 weeks

期刊介绍： Adipocyte recognizes that the adipose tissue is the largest endocrine organ in the body, and explores the link between dysfunctional adipose tissue and the growing number of chronic diseases including diabetes, hypertension, cardiovascular disease and cancer. Historically, the primary function of the adipose tissue was limited to energy storage and thermoregulation. However, a plethora of research over the past 3 decades has recognized the dynamic role of the adipose tissue and its contribution to a variety of physiological processes including reproduction, angiogenesis, apoptosis, inflammation, blood pressure, coagulation, fibrinolysis, immunity and general metabolic homeostasis. The field of Adipose Tissue research has grown tremendously, and Adipocyte is the first international peer-reviewed journal of its kind providing a multi-disciplinary forum for research focusing exclusively on all aspects of adipose tissue physiology and pathophysiology. Adipocyte accepts high-profile submissions in basic, translational and clinical research.