Amorphous Cu/Fe nanoparticles with tandem intracellular and extracellular electron capacity for enhancing denitrification performance and recovery of co-contaminant suppressed denitrification

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING Bioresource Technology Pub Date : 2024-11-13 DOI:10.1016/j.biortech.2024.131812

Jinming Fu , Tingting Guo , Haibo Li , Wenli Liu , Zhi Chen , Xiaoping Wang , Jianbo Guo

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Abstract

In this study, a functionally stable insoluble Cu/Fe nanoparticles (Cu/Fe NPs) were synthesized and applied denitrification with different contaminants. The results showed that 50 mg/L Cu/Fe NPs increased NO₃^–-N reduction rate up to 14.3 mg/(L·h) about 3 folds compared with the control system (4.7 mg/(L·h)), and Cu/Fe NPs exhibited excellent restorative effects on NO₃^–-N reduction under the stress of Cd²⁺, Nitrovin and Methyl Orange. Meanwhile, electrochemical analyses, enzyme activities, and related genes abundance together showed that pilus, cytochrome c and flavin mononucleotide were electron carriers to tandem extracellular and intracellular, increasing electron flux acting on NO₃^–-N in the respiratory chain. Metagenomic sequencing showed that microbial metabolic activity, electroactive bacteria (EAB) abundance with bi-directional electron transfer and Cu/Fe-compatible bacterial abundance were increased. Furthermore, denitrification performance was maintained by establishing C-EAB-Cu/Fe NPs cycling network. This study provided insights and applications for expanding the use of insoluble mediators in denitrification systems.

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具有胞内和胞外电子串联能力的无定形铜/铁纳米粒子，用于提高反硝化性能和恢复被共污染物抑制的反硝化。

本研究合成了一种功能稳定的不溶性铜/铁纳米粒子（Cu/Fe NPs），并将其应用于不同污染物的脱硝过程。结果表明，与对照体系（4.7 mg/(L-h)）相比，50 mg/L Cu/Fe NPs可将NO3--N的还原率提高到14.3 mg/(L-h)，约为对照体系的3倍，而且在Cd2+、硝基乙烯和甲基橙的胁迫下，Cu/Fe NPs对NO3--N的还原具有良好的恢复作用。同时，电化学分析、酶活性和相关基因丰度共同表明，柔毛、细胞色素c和黄素单核苷酸是串联细胞外和细胞内的电子载体，增加了呼吸链中作用于NO3--N的电子通量。元基因组测序显示，微生物代谢活性、具有双向电子传递功能的电活性细菌（EAB）数量和铜/铁兼容细菌数量均有所增加。此外，通过建立 C-EAB-Cu/Fe NPs 循环网络，反硝化性能得以保持。这项研究为扩大不溶性介质在反硝化系统中的使用提供了启示和应用。

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来源期刊

Bioresource Technology 工程技术-能源与燃料

CiteScore

20.80

自引率

19.30%

发文量

2013

审稿时长

12 days

期刊介绍： Bioresource Technology publishes original articles, review articles, case studies, and short communications covering the fundamentals, applications, and management of bioresource technology. The journal seeks to advance and disseminate knowledge across various areas related to biomass, biological waste treatment, bioenergy, biotransformations, bioresource systems analysis, and associated conversion or production technologies. Topics include: • Biofuels: liquid and gaseous biofuels production, modeling and economics • Bioprocesses and bioproducts: biocatalysis and fermentations • Biomass and feedstocks utilization: bioconversion of agro-industrial residues • Environmental protection: biological waste treatment • Thermochemical conversion of biomass: combustion, pyrolysis, gasification, catalysis.