Comparison analysis of ABCG subfamily in bamboo and the potential function of PeABCG15 in monolignol transport

IF 6.1 2区生物学 Q1 PLANT SCIENCES Plant Physiology and Biochemistry Pub Date : 2024-11-06 DOI:10.1016/j.plaphy.2024.109278

Hui Li , Ziyang Li , Kebin Yang , Zeming Lin , Chenglei Zhu , Yan Liu , Zhimin Gao

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Abstract

Lignin is a principal component of secondary cell wall and plays vital roles in various biological processes. In this study, 68 and 42 members of ABC transporter G subfamily (ABCG) were identified in Bambusa amplexicaulis and Olyra latifolia, which were less than that of 77 in moso bamboo (Phyllostachys edulis). Collinearity analysis showed that ABCGs had undergone robust purifying selection with lower functional differentiation. These ABCGs were clustered into two clades of WBC and PDR. Notably, PeABCG15 was highly expressed with the lignification of bamboo shoot. The WGCNA revealed that PeABCG15 was co-expressed with eight MYB genes, among which PeMYB203 was able to activate PeABCG15 validated by Y1H, DLR, and GUS assays. Furthermore, over-expressing PeABCG15 significantly enhanced the content of lignin and the expression levels of monolignol biosynthetic genes in Arabidopsis thaliana, conferring improved tolerance to exogenous coniferyl alcohol. Collectively, our findings elucidated the prospective contribution of PeABCG15 to monolignol transport, providing insights into the lignin biosynthesis mechanism in bamboo.

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竹子中 ABCG 亚家族的比较分析以及 PeABCG15 在单木质素转运中的潜在功能。

木质素是次生细胞壁的主要成分，在各种生物过程中发挥着重要作用。本研究在文竹和毛竹中分别发现了 68 个和 42 个 ABC 转运体 G 亚家族成员，少于毛竹中的 77 个。共线性分析表明，ABCGs经历了强有力的纯化选择，功能分化程度较低。这些 ABCGs 被聚类为 WBC 和 PDR 两个支系。值得注意的是，PeABCG15 在竹笋木质化过程中高表达。WGCNA显示，PeABCG15与8个MYB基因共表达，其中PeMYB203能够激活PeABCG15，Y1H、DLR和GUS实验验证了这一点。此外，过表达 PeABCG15 能显著提高拟南芥中木质素的含量和单木质素生物合成基因的表达水平，从而提高拟南芥对外源针叶醇的耐受性。总之，我们的研究结果阐明了PeABCG15对单木酚转运的预期贡献，为竹子的木质素生物合成机制提供了启示。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.