Prevalence, antimicrobial resistance, virulence gene distribution and SCCmec typing of methicillin-resistant Staphylococcus aureus isolated from raw milk and dairy products.

IF 1.3 Q4 MICROBIOLOGY Iranian Journal of Microbiology Pub Date : 2024-10-01 DOI:10.18502/ijm.v16i5.16793

Najmeh Vahed Dehkordi, Ebrahim Rahimi, Noosha Zia Jahromi

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Abstract

Background and objectives: Researchers have focused on Staphylococcus aureus because it is transmitted through food, such as milk and dairy products, and causes human diseases. Prevalence, antimicrobial resistance, presence, and distribution of methicillin-resistant S. aureus (MRSA) virulence genes isolated from raw milk and dairy products were evaluated.

Materials and methods: 300 samples of dairy products were collected from Shahrekord, Iran. S. aureus was identified using biochemical tests and screened for sensitivity to 13 antibiotics to identify resistance genes. In addition, SCCmec typing was performed.

Results: Out of 300, S. aureus was found in 82 samples. Raw milk had the highest contamination with S. aureus (60 of 82), followed by cheese (15 of 82), and butter (7 of 82). At least one resistance gene was present in every isolate of S. aureus. Virulence factors and enterotoxin-coding genes, such as sea, seb, sec, and sed were highly distributed.

Conclusion: The results of this study revealed the presence of toxin-producing MRSA strains in raw milk and dairy products. MRSA in dairy farms is an important risk factor for the spread of staphylococcal infections; therefore, further studies are needed to find strategies for controlling the presence of S. aureus, especially MRSA, in dairy products.

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从生乳和乳制品中分离出的耐甲氧西林金黄色葡萄球菌的流行率、抗菌药耐药性、毒力基因分布和 SCCmec 分型。

背景和目的：研究人员关注金黄色葡萄球菌，因为它通过牛奶和乳制品等食物传播并导致人类疾病。材料和方法：从伊朗沙赫雷科德收集了 300 份乳制品样本。使用生化测试鉴定金黄色葡萄球菌，并筛查其对 13 种抗生素的敏感性，以确定耐药基因。此外，还进行了 SCCmec 分型：结果：在 300 个样本中，有 82 个样本发现了金黄色葡萄球菌。生牛奶中的金黄色葡萄球菌含量最高（82 个样本中有 60 个），其次是奶酪（82 个样本中有 15 个）和黄油（82 个样本中有 7 个）。每个金黄色葡萄球菌分离物中至少存在一种抗性基因。毒力因子和肠毒素编码基因（如 sea、seb、sec 和 sed）分布广泛：本研究结果显示，生奶和乳制品中存在产毒 MRSA 菌株。奶牛场中的 MRSA 是葡萄球菌感染传播的一个重要风险因素；因此，需要进一步研究以找到控制奶制品中金黄色葡萄球菌（尤其是 MRSA）存在的策略。

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来源期刊

Iranian Journal of Microbiology MICROBIOLOGY-

CiteScore

2.40

自引率

7.10%

发文量

审稿时长

12 weeks

期刊介绍： The Iranian Journal of Microbiology (IJM) is an international, multi-disciplinary, peer-reviewed journal that provides rapid publication of the most advanced scientific research in the areas of basic and applied research on bacteria and other micro-organisms, including bacteria, viruses, yeasts, fungi, microalgae, and protozoa concerning the development of tools for diagnosis and disease control, epidemiology, antimicrobial agents, clinical microbiology, immunology, Genetics, Genomics and Molecular Biology. Contributions may be in the form of original research papers, review articles, short communications, case reports, technical reports, and letters to the Editor. Research findings must be novel and the original data must be available for review by the Editors, if necessary. Studies that are preliminary, of weak originality or merely descriptive as well as negative results are not appropriate for the journal. Papers considered for publication must be unpublished work (except in an abstract form) that is not under consideration for publication anywhere else, and all co-authors should have agreed to the submission. Manuscripts should be written in English.