Sugar Cane Glycoproteins: an Alternative to Improve the Production of Xanthan Gum by Xanthomonas albilineans

Rocío Santiago, Mônica Cristina Barroso Martins, María Estrella Legaz, Carlos Vicente, Nicácio H. da Silva, Esteban Vidal and Eugênia C. Pereira*, 
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Abstract

Xanthomonas albilineans, although it is an essential phytopathogen for sugar cane, is capable of producing xanthan gum (XG). This macromolecule is used in the food, pharmaceutical, and gas production industries, but its production is expensive when considering the substrate and its purity. On the other hand, the possibility of using the material for disposal, such as the infected sugar cane stalk, can be attractive since this substrate can produce XG and has no value for cultivation. Thus, we describe the possibility of its production in different culture media using immobilized cells from sugar cane stalk tissues supplemented with glycoproteins or sucrose. Using molecular patterns associated with bacterial pathogens (PAMPs), the presence of gum in isopropyl alcohol precipitate is obtained after incubation and culture improvement. This production model describes a new possibility of gum production, contributing to the potential for sustainability and diversification of the sugar cane industry.

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甘蔗糖蛋白:改善白化黄单胞菌生产黄原胶的替代品
黄单胞菌(Xanthomonas albilineans)虽然是甘蔗的重要植物病原体,但也能生产黄原胶(XG)。这种大分子用于食品、制药和天然气生产行业,但考虑到基质及其纯度,其生产成本较高。另一方面,利用受感染的甘蔗茎等材料进行处理的可能性也很有吸引力,因为这种基质可以生产 XG,而且没有种植价值。因此,我们介绍了利用来自甘蔗茎组织的固定化细胞并辅以糖蛋白或蔗糖在不同培养基中生产 XG 的可能性。利用与细菌病原体相关的分子模式(PAMPs),经过孵育和培养改良后,在异丙醇沉淀中发现了树胶的存在。这种生产模式描述了一种生产树胶的新可能性,为甘蔗产业的可持续发展和多样化潜力做出了贡献。
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