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Nutritional Composition and Content of Bioactive Compounds in Field Pea and Chickpea Varieties as Functional Raw Material for the Food Supply Chain. 作为食品供应链功能性原料的大田豌豆和鹰嘴豆品种的营养成分及生物活性化合物含量
IF 2.8 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-12-03 eCollection Date: 2025-12-19 DOI: 10.1021/acsfoodscitech.5c00941
Manuel Martoccia, Matteo Donna, Paolo Colombatto, Jean Daniel Coïsson, Fabiano Travaglia, Massimo Blandino

Despite the increasing demand for plant-based foods that has renewed interest in pulses as functional ingredients, limited studies have compared the compositional variability among commercial chickpea and field pea cultivars. This study compared the compositional variability of 12 field pea and 11 chickpea cultivars cultivated under identical conditions in Northwest Italy. Comprehensive nutritional and phytochemical profiles, including macronutrients, carotenoids, phenolics, flavonoids, and antioxidant activities, were assessed. Among field peas, the Bluemoon cv showed the highest starch content (57.5 g 100-1 g d.w.), supporting its suitability for extrusion-based products, while Angelus exhibited the highest phenolic content (154.2 mg kg-1 d.w.) and antioxidant activity. In chickpeas, the desi cv Nero stood out for its elevated dietary fiber (21.5 g 100 g-1 d.w.), phenolics (37.2 mg kg-1 d.w.), and antioxidant capacity. These findings highlight the cultivar selection value to improve the nutritional and functional properties of pulse-based foods and support sustainable innovation in food-product development.

尽管对植物性食品的需求不断增加,重新燃起了人们对豆类作为功能性成分的兴趣,但有限的研究比较了商业鹰嘴豆和田间豌豆品种之间的成分差异。本研究比较了意大利西北部在相同条件下栽培的12个大田豌豆和11个鹰嘴豆品种的组成变异。全面的营养和植物化学特征,包括宏量营养素,类胡萝卜素,酚类物质,类黄酮和抗氧化活性,进行了评估。在大田豌豆中,蓝月豌豆的淀粉含量最高(57.5 g 100-1 g d.w.),支持其适合挤出产品,而Angelus的酚含量最高(154.2 mg kg-1 d.w.)和抗氧化活性最高。在鹰嘴豆中,Nero品种因其高膳食纤维(21.5 g 100 g-1 d.w.)、酚类物质(37.2 mg kg-1 d.w.)和抗氧化能力而脱颖而出。这些发现突出了豆类食品的品种选择价值,可以改善豆类食品的营养和功能特性,并支持食品开发的可持续创新。
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引用次数: 0
From Waste to Worth: Transforming Winemaking Residues into High-Value Ingredients. 从废物到价值:将酿酒残留物转化为高价值成分。
IF 2.8 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-11-09 eCollection Date: 2025-11-21 DOI: 10.1021/acsfoodscitech.5c00519
Daniela Ratto, Valeria Cavalloro, Elisabetta Tumminelli, Irene Soffientini, Emanuela Martino, Daniela Rossi, Simona Collina, Paola Rossi

The wine industry generates significant amounts of waste, including vine shoots, grape pomace, and seeds. Traditionally regarded as waste, these materials are now recognized for their richness in bioactive compounds such as polyphenols, dietary fibers, and antioxidants, offering numerous health and environmental benefits. This review explores the potential applications of vinification residues and vine cultivation residues in the food, pharmaceutical, and cosmetic industries, highlighting recent advancements in these fields. The valorization of these winemaking wastes shifts toward a circular and sustainable economy, reducing environmental impact while creating economic opportunities. The need for optimizing waste management practices in the wine sector to foster sustainability and innovation is evidenced by the numerous patents filed in recent years. Future research should focus on scalable technologies, regulatory harmonization, and interdisciplinary approaches to fully integrate winemaking waste into high-value production chains.

葡萄酒行业产生了大量的废物,包括葡萄藤芽、葡萄渣和种子。传统上,这些材料被认为是废物,现在人们认识到它们富含生物活性化合物,如多酚、膳食纤维和抗氧化剂,对健康和环境有许多好处。本文综述了酿酒残留物和葡萄栽培残留物在食品、制药和化妆品行业的潜在应用,重点介绍了这些领域的最新进展。这些酿酒废料的增值转向循环和可持续经济,减少对环境的影响,同时创造经济机会。近年来申请的众多专利证明了优化葡萄酒行业废物管理实践以促进可持续性和创新的必要性。未来的研究应该集中在可扩展的技术、监管协调和跨学科的方法上,以充分将酿酒废物整合到高价值的生产链中。
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引用次数: 0
Impact of Cold Plasma Treatment on the Shelf Life and Metabolite Profiles of Strawberries during Storage. 冷等离子体处理对草莓贮藏期和代谢物谱的影响
IF 2.8 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-10-03 eCollection Date: 2025-10-17 DOI: 10.1021/acsfoodscitech.5c00700
D Abouelenein, G G Gebremical, S Tappi, B Cellini, F Shanbeh Zadeh, A M Mustafa, G Caprioli, Nadezhda Frolova, A Soboleva, A Frolov, L Vannini, P Rocculi, S Vittori

Strawberries are abundant in bioactive compounds and serve as a significant source of ascorbic acid. However, their shelf life is notoriously short due to their high sensitivity to environmental conditions and susceptibility to microbial contamination. The growing demand for ready-to-eat products among consumers presents challenges related to food preservation, especially with increasing food losses due to microbial spoilage. Various strategies have been explored to address these issues, one of which is atmospheric cold plasma. A surface dielectric barrier discharge cold plasma (CP) treatment was applied to fresh strawberries (output voltage: 6 kV, frequency: 23 kHz) for 30 min. Spoilage microbial populations, quality, and primary and secondary metabolite profiles were evaluated during storage at 4 °C. Cold plasma treatment resulted in significant reductions in the counts of the main spoilage microbial groups, which showed delayed and limited growth compared to the untreated fruits. LC-MS/MS analysis revealed the preservation of the total phenolic profile, along with a significant increase in total phenolic acids (from 614.2 to 784.4 mg/kg) and total flavonols (from 145.2 to 196.4 mg/kg) immediately after treatment. An increase in the levels of specific polyphenols and antioxidant activity was observed. Although ascorbic acid decreased after treatment, greater stability was noted during storage. In conclusion, CP can preserve fruit quality and extend the shelf life of fresh strawberries without adversely affecting their physical properties.

草莓富含生物活性化合物,是抗坏血酸的重要来源。然而,由于它们对环境条件的高度敏感性和对微生物污染的敏感性,它们的保质期是出了名的短。消费者对即食产品的需求日益增长,对食品保存提出了挑战,特别是由于微生物腐败造成的食品损失日益增加。已经探索了各种策略来解决这些问题,其中之一是大气冷等离子体。采用表面介质阻挡放电冷等离子体(CP)处理新鲜草莓,输出电压为6 kV,频率为23 kHz,处理时间为30 min。在4°C储存期间评估腐败微生物种群,质量和初级和次级代谢物谱。冷等离子体处理导致主要腐败微生物群的数量显著减少,与未处理的水果相比,这些微生物群表现出生长延迟和限制。LC-MS/MS分析显示,处理后总酚酸含量从614.2 mg/kg增加到784.4 mg/kg,总黄酮醇含量从145.2 mg/kg增加到196.4 mg/kg。观察到特定多酚水平和抗氧化活性的增加。虽然抗坏血酸在处理后减少,但在储存期间注意到更大的稳定性。综上所述,CP可以在不影响草莓物理特性的前提下,保持草莓的品质,延长草莓的保质期。
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引用次数: 0
Ham Yeasts: Exploring Mycoprotein Potential Production of Yeasts Isolated from Spanish Dry-Cured Ham. 火腿酵母:探索从西班牙干腌火腿中分离的酵母的真菌蛋白生产潜力。
IF 2.8 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-09-26 eCollection Date: 2025-10-17 DOI: 10.1021/acsfoodscitech.5c00671
Noelia Viveros-Lizondo, Beatriz García-Béjar, Elena Coso-Cuevas, Almudena Soriano, María Arévalo-Villena

Ripening conditions of dry-cured ham provide a suitable environment for microbial growth. Although salts such as sodium chloride, nitrite, and nitrate act as inhibitory agents, certain microorganisms, particularly yeasts, can still develop. This study evaluates the biodiversity and biotechnological traits of yeasts isolated from Spanish dry-cured hams, comparing natural and controlled drying rooms and assessing their potential for protein production. Samples were collected from five drying rooms, including both Serrano and Iberian hams as well as ham hangers and air. The average yeast population was 5.44 ± 1.29 log CFU/cm2, with no growth detected in air samples. Non-Saccharomyces species were more prevalent than Saccharomyces, with Debaryomyces hansenii and Yarrowia lipolytica being the most dominant due to their strong proteolytic activity, which contributes to ham flavor and texture. Natural drying rooms exhibited greater yeast diversity and higher counts. Selected yeast strains were evaluated for their potential as mycoprotein sources through kinetic and protein production analyses. Debaryomyces hansenii showed the highest protein content (495.11 ± 22.19 mg/g dry weight), making it a promising candidate for low-animal-protein meat alternatives.

干腌火腿的成熟条件为微生物的生长提供了适宜的环境。虽然盐如氯化钠、亚硝酸盐和硝酸盐起抑制作用,但某些微生物,特别是酵母菌仍然可以生长。本研究评估了从西班牙干腌火腿中分离的酵母的生物多样性和生物技术特性,比较了自然干燥室和控制干燥室,并评估了它们生产蛋白质的潜力。样本从五个干燥室收集,包括塞拉诺火腿和伊比利亚火腿以及火腿架和空气。平均酵母数量为5.44±1.29 log CFU/cm2,空气样品中未检测到生长。非酵母菌种类比酵母菌多,其中汉斯德巴酵母(Debaryomyces hansenii)和多脂耶氏酵母(Yarrowia lipolytica)最占优势,因为它们具有较强的蛋白质水解活性,这有助于火腿的风味和质地。自然干燥室表现出更大的酵母多样性和更高的计数。通过动力学分析和蛋白质生产分析,对选定的酵母菌株作为真菌蛋白来源的潜力进行了评价。汉氏德巴氏菌的蛋白质含量最高(495.11±22.19 mg/g干重),是低动物蛋白肉类的理想替代品。
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引用次数: 0
Solid-State Fermentation-Assisted Extraction for Enhancing Recovery of Polyphenol-Rich Extracts from Pomegranate Peels (Punica granatum L.): Aligning with OIV Standards for Oenotannins 固态发酵辅助提取法提高石榴皮中富含多酚的提取物的回收率:与OIV标准酒单宁的一致性
IF 2.8 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-09-10 DOI: 10.1021/acsfoodscitech.5c00729
Silvia D’Agostino, Mohamad Khatib, Lorenzo Cecchi*, Valentina Canuti, Paola Domizio, Francesco Maioli, Nadia Mulinacci and Bruno Zanoni, 

Pomegranate peel is a byproduct of juice production rich in distinctive ellagitannins characterized by the gallagyl group. The resulting extract may be used as an oenological tannin, provided that it meets the minimum polyphenol concentration of 65% w/w set by the International Organization of Vine and Wine (OIV) and contains low levels of pectin. The aim was to improve conventional heat-assisted extraction (HAE) to obtain extracts enriched in polyphenols and lowered in pectic polysaccharides. Therefore, HAE, Low-Temperature Hydroalcoholic Extraction (LTHE) and Solid-State Fermentation with Saccharomyces cerevisiae sp. combined with LTHE (SSF + LTHE) were compared. LTHE yielded the lowest polysaccharide content (0.2–1% w/w), whereas SSF + LTHE allowed for an average 40% increase of polyphenols and a 70% reduction in polysaccharides compared to HAE. Only SSF + LTHE allowed the recovery of total polyphenol percentages above the OIV threshold, highlighting the promising potential of solid-state fermentation-assisted extraction to produce polyphenol-rich extracts from pomegranate peel.

石榴皮是果汁生产的副产品,富含独特的鞣花单宁,其特征是gallagyl组。所得到的提取物可以用作酿酒单宁,前提是它符合国际葡萄和葡萄酒组织(OIV)规定的65% w/w的最低多酚浓度,并且含有低水平的果胶。目的是改进传统的热辅助提取(HAE),以获得富含多酚和降低果胶多糖的提取物。因此,我们比较了HAE、低温氢醇提取(LTHE)和酵母固态发酵(SSF + LTHE)。LTHE的多糖含量最低(0.2-1% w/w),而与HAE相比,SSF + LTHE的多酚含量平均增加40%,多糖含量平均减少70%。只有SSF + LTHE允许总多酚百分比的回收率超过OIV阈值,突出了固态发酵辅助提取生产富含多酚的石榴皮提取物的潜力。
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引用次数: 0
Enhancing Structural Stability and Reducing Glycemic Response in Sweet Potato Starch Gels: Chitosan and Xanthan Gum via Electrostatic Interactions and Hydrogen Bonding 增强甘薯淀粉凝胶结构稳定性和降低血糖反应:壳聚糖和黄原胶通过静电相互作用和氢键
IF 2.8 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-09-08 DOI: 10.1021/acsfoodscitech.5c00633
Pasdar Hussain, Miao Zhang*, Ling Zhang, Hongnan Sun*, Feihu Gao and Taihua Mu*, 

To develop sweet potato starch (SPS) gels with high stability and a low estimated glycemic index (eGI), effects of xanthan gum (XG), carboxymethyl cellulose (CMC), chitosan (CHI), microcrystalline cellulose (MCC), and polydextrose (PDX) on the structure, rheological properties, moisture distribution, and in vitro digestibility of SPS gels were investigated. Rheological analysis revealed a significantly reduced z′ value of XG, CMC, CHI, and MCC, while it revealed an increased K value of fresh SPS gels, suggesting stable network formation. Fourier transform infrared (FTIR) spectra demonstrated that CHI presented the highest R1047/1022 value (1.80) and the lowest R1022/995 value (0.43) after retrogradation, followed by XG, suggesting more ordered structures. CHI yielded the lowest eGI (50.54) in retrograded gels, whereas XG led to a lower eGI in both fresh (55.34) and retrograded (54.17) states. This difference might be attributed to electrostatic interactions and hydrogen bonding between CHI and SPS, as well as strong hydrogen bonds between XG and SPS. These findings highlight CHI and XG as promising choices for designing functional starch-based foods.

为了研制高稳定性、低血糖指数(eGI)的甘薯淀粉(SPS)凝胶,研究了黄原胶(XG)、羧甲基纤维素(CMC)、壳聚糖(CHI)、微晶纤维素(MCC)和聚葡萄糖(PDX)对SPS凝胶结构、流变性能、水分分布和体外消化率的影响。流变学分析显示,XG、CMC、CHI和MCC的z′值显著降低,而新鲜SPS凝胶的K′值增加,表明网络形成稳定。傅里叶变换红外光谱(FTIR)表明,CHI的R1047/1022值最高(1.80),R1022/995值最低(0.43),其次是XG,表明其结构更有序。CHI在逆行凝胶中产生最低的eGI(50.54),而XG在新鲜和逆行凝胶中产生较低的eGI(55.34)。这种差异可能归因于CHI和SPS之间的静电相互作用和氢键,以及XG和SPS之间的强氢键。这些发现强调CHI和XG是设计功能性淀粉基食品的有希望的选择。
{"title":"Enhancing Structural Stability and Reducing Glycemic Response in Sweet Potato Starch Gels: Chitosan and Xanthan Gum via Electrostatic Interactions and Hydrogen Bonding","authors":"Pasdar Hussain,&nbsp;Miao Zhang*,&nbsp;Ling Zhang,&nbsp;Hongnan Sun*,&nbsp;Feihu Gao and Taihua Mu*,&nbsp;","doi":"10.1021/acsfoodscitech.5c00633","DOIUrl":"https://doi.org/10.1021/acsfoodscitech.5c00633","url":null,"abstract":"<p >To develop sweet potato starch (SPS) gels with high stability and a low estimated glycemic index (eGI), effects of xanthan gum (XG), carboxymethyl cellulose (CMC), chitosan (CHI), microcrystalline cellulose (MCC), and polydextrose (PDX) on the structure, rheological properties, moisture distribution, and <i>in vitro</i> digestibility of SPS gels were investigated. Rheological analysis revealed a significantly reduced <i>z</i>′ value of XG, CMC, CHI, and MCC, while it revealed an increased <i>K</i> value of fresh SPS gels, suggesting stable network formation. Fourier transform infrared (FTIR) spectra demonstrated that CHI presented the highest <i>R</i><sub>1047/1022</sub> value (1.80) and the lowest <i>R</i><sub>1022/995</sub> value (0.43) after retrogradation, followed by XG, suggesting more ordered structures. CHI yielded the lowest eGI (50.54) in retrograded gels, whereas XG led to a lower eGI in both fresh (55.34) and retrograded (54.17) states. This difference might be attributed to electrostatic interactions and hydrogen bonding between CHI and SPS, as well as strong hydrogen bonds between XG and SPS. These findings highlight CHI and XG as promising choices for designing functional starch-based foods.</p>","PeriodicalId":72048,"journal":{"name":"ACS food science & technology","volume":"5 9","pages":"3577–3589"},"PeriodicalIF":2.8,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145094565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Fabrication of Silver Nanoparticles in PVA Polymeric Films for Achieving Supreme Antimicrobial Nanocomposites: A Smarter Approach toward Food Packaging 在聚乙烯醇聚合膜中制备银纳米粒子以获得最高的抗菌纳米复合材料:一种更智能的食品包装方法
IF 2.8 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-09-03 DOI: 10.1021/acsfoodscitech.5c00501
Amreen Firoz, Anuja Chatterjee, Arpan Kool, Namrata Das, Debmalya Sarkar, Somtirtha Kool Banerjee* and Sukhen Das*, 

The extravagant use of traditional plastic packaging materials has increased the problem of food spoilage and toxicity in recent days. Nontoxic and environmentally benign alternatives to plastic materials for the packaging of foods are readily required. Silver nanoparticles (AgNPs) impregnated polyvinyl alcohol (PVA) thin films were fabricated after the synthesis of AgNPs using a simple chemical reduction method. The crystal structure, functional groups, microstructure, distribution, and absorbance pattern have been investigated using X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, field emission scanning electron microscopy (FESEM), and UV–vis spectroscopy. The antibacterial activity of the PVA-AgNP nanocomposite was investigated by observing the width of the zone of inhibition, which is the area around the diffused sample on a culture plate with prevented bacterial growth. The MIC was determined using a dilution assay while maintaining a positive and negative control. The results of the experimental investigations confirmed the formation of AgNPs and the PVA-AgNP nanocomposite. The average length of the rod-shaped AgNPs was 5 μm with an average diameter of ∼90 nm. The nanocomposite showcased excellent antimicrobial activity against both Gram-positive Bacillus subtilis and Gram-negative Pseudomonas aeruginosa and Escherichia coli microorganisms, which was confirmed by the wide zone of inhibition with no bacterial growth. A dilution assay was set up to determine the MIC of AgNPs, which was found to be 20 μL/mL. In addition to the antimicrobial properties, the PVA-AgNP exhibited good water vapor retention ability for 5 days as well as preservation of ascorbic acid content of the nutritious foods after 14 days of packaging. Hence, the PVA-AgNP nanocomposite film fabricated in this work possesses supreme antimicrobial properties and acts as a potentially active food packaging material, substituting the toxic traditional plastic packaging materials.

近年来,传统塑料包装材料的过度使用增加了食品腐败和毒性问题。无毒和环保替代品的塑料材料包装食品是很容易需要的。采用简单的化学还原法制备了银纳米粒子(AgNPs)浸渍聚乙烯醇(PVA)薄膜。利用x射线衍射(XRD)、傅里叶变换红外光谱(FTIR)、场发射扫描电子显微镜(FESEM)和紫外可见光谱(UV-vis)对其晶体结构、官能团、微观结构、分布和吸光度进行了研究。通过观察抑制区宽度来研究PVA-AgNP纳米复合材料的抗菌活性,该抑制区是在培养板上扩散样品周围阻止细菌生长的区域。MIC采用稀释法测定,同时保持阳性和阴性对照。实验研究结果证实了agnp和PVA-AgNP纳米复合材料的形成。杆状AgNPs的平均长度为5 μm,平均直径为~ 90 nm。该纳米复合材料对革兰氏阳性枯草芽孢杆菌、革兰氏阴性铜绿假单胞菌和大肠杆菌均表现出良好的抑菌活性,抑菌范围广,无细菌生长。采用稀释法测定AgNPs的MIC值为20 μL/mL。PVA-AgNP除具有抗菌性能外,还具有良好的5天的水汽保持能力,并能在包装14天后保存营养食品的抗坏血酸含量。因此,本研究制备的PVA-AgNP纳米复合膜具有极高的抗菌性能,可作为潜在的活性食品包装材料,取代有毒的传统塑料包装材料。
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引用次数: 0
First-Order Degradation Kinetics of Phenolic Compounds and Antioxidant Properties of Fresh and Enzymatically Hydrolyzed Seriguela Pulp (Spondias purpurea L.) 新鲜和酶解木浆酚类化合物的一级降解动力学及抗氧化性能
IF 2.8 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-09-02 DOI: 10.1021/acsfoodscitech.5c00554
Mariana de Oliveira Silva*,  and , Ruann Janser Soares de Castro*, 

This study reported the first kinetic assessment of thermal degradation of phenolic compounds and antioxidant activity in fresh and enzymatically hydrolyzed seriguela (Spondias purpurea L.) pulp. Hydrolyzed pulp had 75% higher total phenolics but was more thermally sensitive, with half-life (t1/2) values from 151.8 to 43.2 min versus 297.7 to 73.3 min in the control (40–70 °C). Antioxidant activity (ABTS, DPPH, FRAP) significantly (p < 0.05) decreased with temperature. At 70 °C, ABTS activity dropped 48% in control and 70% in hydrolyzed pulp; FRAP values fell by up to 87% in control, while hydrolyzed pulp lost 50%. Postpasteurization HPLC showed gallic acid as predominant in control (22% reduction) and vanillic acid in hydrolyzed pulp, which was fully degraded. Ferulic acid appeared only after enzymatic hydrolysis, indicating enhanced extraction. These findings help optimize thermal processing to better preserve bioactive compounds in fruit products.

本研究首次报道了新鲜和酶解丝瓜(Spondias purpurea L.)果肉中酚类化合物热降解和抗氧化活性的动力学评价。水解后的纸浆总酚含量高出75%,但对热敏性更强,半衰期(t1/2)值为151.8 ~ 43.2 min,而对照组(40-70°C)为297.7 ~ 73.3 min。抗氧化活性(ABTS、DPPH、FRAP)随温度升高显著降低(p < 0.05)。70℃时,ABTS活性下降48%,水解纸浆下降70%;控制组的FRAP值下降了87%,而水解纸浆下降了50%。巴氏杀菌后高效液相色谱显示,未水解果肉中没食子酸含量最高(还原22%),而水解果肉中香草酸含量最高(完全降解)。阿魏酸仅在酶解后出现,表明提取增强。这些发现有助于优化热加工,以更好地保存水果制品中的生物活性化合物。
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引用次数: 0
Comparative Evaluation of Plant Protein Isolate Blends and Commercial Isolates: Structural, Digestive, and Functional Insights 植物蛋白分离混合物和商业分离物的比较评价:结构、消化和功能见解
IF 2.8 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-09-02 DOI: 10.1021/acsfoodscitech.5c00467
Brijesh Lekhak, Minakshi Dutta, Rama Prashat G, Suneha Goswami, Ranjeet Ranjan Kumar, Navita Bansal, Nagesh C R, Satish M, Shalini Gaur Rudra, Somnath Mandal, S. V. Ramesh, Vignesh Muthusamy, Shivani Nagar, Aruna Tyagi* and Vinutha T*, 

Three plant protein isolate (PlPI) blends were formulated using mung bean, chickpea, sesame, peanut, and spirulina, and compared with seven commercial plant protein isolates (CPlPIs) to evaluate nutritional, structural, digestive, and functional properties. PlPI blends showed superior essential amino acid content, higher soluble protein, and in vitro protein digestibility (73–82%) compared to CPlPIs (33–64%). The improved digestibility was linked to a higher α-helix/β-sheet ratio (24.1%), as confirmed by FTIR, and favorable microstructures observed via SEM and TEM. SDS-PAGE revealed greater levels of β-conglycinin and glycinin in PlPI blends, contributing to improved emulsion stability. Overall, PlPI blends demonstrated enhanced solubility, digestibility, and emulsifying capacity due to synergistic effects of individual proteins. These results highlight PlPI blends as promising, sustainable alternatives for inclusion in protein-rich functional foods and beverages.

采用绿豆、鹰嘴豆、芝麻、花生和螺旋藻配制了3种植物分离蛋白(PlPI)混合物,并与7种商品植物分离蛋白(cplpi)进行了营养、结构、消化和功能特性的比较。与cplpi(33-64%)相比,PlPI混合物具有更高的必需氨基酸含量,更高的可溶性蛋白质和体外蛋白质消化率(73-82%)。红外光谱(FTIR)和扫描电镜(SEM)、透射电镜(TEM)观察到良好的微观结构,证实了α-螺旋/β-薄片比(24.1%)的提高与消化率的提高有关。SDS-PAGE显示,PlPI共混物中β-甘氨酸和甘氨酸的含量更高,有助于提高乳液的稳定性。总体而言,由于单个蛋白质的协同作用,PlPI共混物表现出更高的溶解度、消化率和乳化能力。这些结果突出了PlPI混合物作为富含蛋白质的功能性食品和饮料中有前途的、可持续的替代品。
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引用次数: 0
Surface Fermentation without Downstream Processing as a Strategy for the Development of Tempeh-Like Vegan Patties 无下游加工的表面发酵:豆豉类素食肉饼的发展策略
IF 2.8 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-09-02 DOI: 10.1021/acsfoodscitech.5c00645
Juliano Lemos Bicas*, Gustavo Aparecido Martins and Holger Zorn, 

Given the growing demand for meat analogues, interest in plant-based and fermentation-based products has risen. Therefore, this paper describes the development of “vegan patties” by surface cultivating the basidiomycete Pleurotus citrinopileatus on crushed wheat for 6 days at 24 °C under dark and static conditions. After mycelial development (without any residues or recovery/purification procedures), the resulting cohesive and fibrous product was further baked or fried. Unfermented or fermented patties, with or without baking or frying, were compared in terms of the protein content and protein quality. Fermentation (with or without further baking) changed the amino acid profile, significantly improving the essential amino acid index and biological value of crushed wheat. These results indicate that surface fermentation with basidiomycetes is a promising strategy for developing nutritious (vegan) meat analogues, particularly for avoiding the costly downstream processing.

鉴于对肉类类似物的需求不断增长,人们对植物性和发酵性产品的兴趣也在上升。因此,本文描述了在24°C的黑暗和静态条件下,在碾碎小麦上表面培养担子菌Pleurotus citrinopileatus 6天的“纯素馅饼”的发展。在菌丝发育后(没有任何残留物或回收/纯化程序),得到的粘性和纤维产品进一步烘烤或油炸。在蛋白质含量和蛋白质质量方面,对未发酵和发酵肉饼进行了比较。发酵(加或不加进一步烘焙)改变了小麦的氨基酸分布,显著提高了小麦的必需氨基酸指数和生物学价值。这些结果表明,担子菌的表面发酵是开发营养(纯素)肉类类似物的一种有前途的策略,特别是避免了昂贵的下游加工。
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引用次数: 0
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