An analysis of the efficacy of universal PCR and BACTEC 9120 BD for identifying bacteremia in pediatrics.

IF 1.7 Q3 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH GMS Hygiene and Infection Control Pub Date : 2024-10-23 eCollection Date: 2024-01-01 DOI:10.3205/dgkh000509

Azam Safarkhani, Fatemeh Shirkavand, Nafiseh Abdollahi, Nazanin Ahari Oskooie, Leila Azimi, Mohammad Rahbar, Abdollah Karimi

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Abstract

Background: Bloodstream infections (BSI) are serious diseases in pediatrics and can increase the rate of morbidity and mortality. Blood culture is time consuming and can have false negative results in some case such as the intracellular or fastidious bacteria. This study aimed to evaluate the PCR against automated blood culture with BACTEC.

Materials and methods: In this observational cross-sectional study the blood samples of hospitalized children in Mofid Children's Hospital with bacteremia signs from February to May 2023 were enrolled. The causative bacteria in bacteremia were identified by phenotypic and PCR methods.

Results: 150 blood samples were enrolled to identify the presence of bacteremia by BACTEC and PCR. 60% and 40% of samples have negative and positive results in both methods, respectively. PCR showed 100% sensitivity and specificity in detecting bacteremia compared to BACTEC. A variety of bacteria were identified by phenotypic and molecular methods and coagulase negative Staphylococcus (CONS) is the most of them.

Conclusion: The rapid and accurate detection of bacterial pathogens with the high sensitivity and specificity compared gold standard method are the most important profits of molecular assay.

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分析通用 PCR 和 BACTEC 9120 BD 在鉴定儿科菌血症方面的功效。

背景：血流感染（BSI）是儿科的严重疾病，会增加发病率和死亡率。血液培养费时费力，而且在某些情况下会出现假阴性结果，如细胞内细菌或苛氧菌。本研究旨在评估 PCR 与 BACTEC 自动血液培养的对比结果：在这项观察性横断面研究中，研究人员采集了莫菲德儿童医院 2023 年 2 月至 5 月期间出现菌血症征兆的住院患儿的血液样本。通过表型和 PCR 方法鉴定了菌血症中的致病菌：结果：共采集了 150 份血液样本，通过 BACTEC 和 PCR 方法确定是否存在菌血症。两种方法分别有 60% 和 40% 的样本结果为阴性和阳性。与 BACTEC 相比，PCR 检测菌血症的灵敏度和特异性均为 100%。通过表型和分子方法鉴定出多种细菌，其中最多的是凝固酶阴性葡萄球菌（CONS）：结论：快速、准确地检测细菌病原体，与金标准方法相比具有较高的灵敏度和特异性，这是分子检测的最大优点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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GMS Hygiene and Infection Control PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH-

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10 weeks