Reduction of Plasmodiophora brassicae infection on Brassica rapa through host-induced gene silencing of two secreted genes.

IF 2.6 2区农林科学 Q2 PLANT SCIENCES Phytopathology Pub Date : 2024-11-19 DOI:10.1094/PHYTO-09-23-0334-R

Hui Yang, Yihan Zhang, Yushan Zhao, Yinping Shu, Yushu Xu, Yi Liu, Junbo Du, Wenming Wang

{"title":"Reduction of Plasmodiophora brassicae infection on Brassica rapa through host-induced gene silencing of two secreted genes.","authors":"Hui Yang, Yihan Zhang, Yushan Zhao, Yinping Shu, Yushu Xu, Yi Liu, Junbo Du, Wenming Wang","doi":"10.1094/PHYTO-09-23-0334-R","DOIUrl":null,"url":null,"abstract":"Clubroot disease caused by the biotrophic pathogen Plasmodiophora brassicae, is one of the most serious threats to cruciferous crops production worldwide. P. brassicae is known for rapid adaptive evolution to overcome resistance in varieties. It is urgent to establish alternative management to control P. brassicae. In this study, we identified two P. brassicae secretory proteins that were up-regulated during infection and effected plant defense. We established a method for transient expression in the roots of seedlings and demonstrated that P. brassicae could take up substances from the environment of root cells. Using a RNA interference (RNAi)-based host-induced gene silencing (HIGS) by expression of hairpin RNAi constructs with sequence homology to P. brassicae effector Pb48 or Pb52 in susceptible Brassica rapa plants enhanced host disease resistance. After silencing these two effectors, the transcription levels of cytokinin biosynthesis gene IPT1 and the regulation gene of auxin homeostasis GH3.5 were down-regulated. These results suggested that RNAi-based HIGS of effectors has a great practical application of improving crop resistance against P. brassicae and can contribute to environmentally sustainable agriculture.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":""},"PeriodicalIF":2.6000,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Phytopathology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1094/PHYTO-09-23-0334-R","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PLANT SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Clubroot disease caused by the biotrophic pathogen Plasmodiophora brassicae, is one of the most serious threats to cruciferous crops production worldwide. P. brassicae is known for rapid adaptive evolution to overcome resistance in varieties. It is urgent to establish alternative management to control P. brassicae. In this study, we identified two P. brassicae secretory proteins that were up-regulated during infection and effected plant defense. We established a method for transient expression in the roots of seedlings and demonstrated that P. brassicae could take up substances from the environment of root cells. Using a RNA interference (RNAi)-based host-induced gene silencing (HIGS) by expression of hairpin RNAi constructs with sequence homology to P. brassicae effector Pb48 or Pb52 in susceptible Brassica rapa plants enhanced host disease resistance. After silencing these two effectors, the transcription levels of cytokinin biosynthesis gene IPT1 and the regulation gene of auxin homeostasis GH3.5 were down-regulated. These results suggested that RNAi-based HIGS of effectors has a great practical application of improving crop resistance against P. brassicae and can contribute to environmentally sustainable agriculture.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

通过宿主诱导的两个分泌基因沉默，减少 Plasmodiophora brassicae 对 Brassica rapa 的感染。

由生物营养型病原体黄铜疫霉（Plasmodiophora brassicae）引起的棒根病是全球十字花科作物生产面临的最严重威胁之一。众所周知，P. brassicae能快速适应进化，克服品种的抗性。当务之急是建立替代管理方法来控制 P. brassicae。在这项研究中，我们发现了两种在感染期间上调并影响植物防御的 P. brassicae 分泌蛋白。我们建立了一种在幼苗根部进行瞬时表达的方法，并证明了黄铜穗虫能从根细胞的环境中吸收物质。通过在易感芸薹属植物中表达与铜绿微囊藻效应子 Pb48 或 Pb52 序列同源的发夹式 RNAi 构建体，使用基于 RNA 干扰（RNAi）的宿主诱导基因沉默（HIGS）技术，增强了宿主的抗病性。沉默这两种效应因子后，细胞分裂素生物合成基因 IPT1 和辅助素平衡调控基因 GH3.5 的转录水平下调。这些结果表明，基于 RNAi 的效应子 HIGS 在提高作物对黄铜穗芥的抗性方面有很大的实际应用价值，并能为环境可持续农业做出贡献。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Phytopathology 生物-植物科学

CiteScore

5.90

自引率

9.40%

发文量

505

审稿时长

4-8 weeks

期刊介绍： Phytopathology publishes articles on fundamental research that advances understanding of the nature of plant diseases, the agents that cause them, their spread, the losses they cause, and measures that can be used to control them. Phytopathology considers manuscripts covering all aspects of plant diseases including bacteriology, host-parasite biochemistry and cell biology, biological control, disease control and pest management, description of new pathogen species description of new pathogen species, ecology and population biology, epidemiology, disease etiology, host genetics and resistance, mycology, nematology, plant stress and abiotic disorders, postharvest pathology and mycotoxins, and virology. Papers dealing mainly with taxonomy, such as descriptions of new plant pathogen taxa are acceptable if they include plant disease research results such as pathogenicity, host range, etc. Taxonomic papers that focus on classification, identification, and nomenclature below the subspecies level may also be submitted to Phytopathology.