An overview of extraction, isolation and characterization techniques of phytocompounds from medicinal plants.

IF 1.9 3区化学 Q3 CHEMISTRY, APPLIED Natural Product Research Pub Date : 2024-11-19 DOI:10.1080/14786419.2024.2426059

Susmita Mondal, Manosi Das, Sudipto Debnath, Biresh Kumar Sarkar, Gajji Babu

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Abstract

Medicinal plants have been used since time immemorial for the treatment of many types of diseases along with epidemics. They show many biological activities like anti-inflammatory, antioxidant, anti-insecticidal, antibiotic, anti-parasitic, anti-hemolytic properties etc. Different types of bioactive compounds, present in the medicinal plants, play a key role in prevention of diseases and also used for manufacturing medicines. Thus, phytochemicals have an immense value to communities worldwide. The present review focused on the extraction, purification and characterisation method for isolation of phytocompounds. Both the conventional like maceration, percolation, digestion, infusion a decoction, soxhlet and reflux, hydro distillation and steam distillation method and modern technique of extraction like ASE, microwave- and ultrasound-assisted extraction, supercritical fluid extraction, enzyme-assisted fluid extraction, pressurised hot water extraction are elaborately explained in the present review. The methodology of purification by LLF & LLP, recrystallization, different chromatographic techniques like TLC, prep-TLC, column chromatography, flash column chromatography, SEC, counter current chromatography, HPLC & prep-GC are also included in the present review. Structure elucidation by UV-Vis spectroscopy, FTIR, NMR spectroscopy, mass spectroscopy and X-ray crystallography are also discussed here.

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药用植物中植物化合物的提取、分离和表征技术概览。

药用植物自古以来就被用于治疗多种疾病和流行病。药用植物具有多种生物活性，如消炎、抗氧化、杀虫、抗生素、抗寄生虫、抗溶血等。药用植物中的各种生物活性化合物在预防疾病和制造药物方面发挥着重要作用。因此，植物化学物质对全球社区具有巨大价值。本综述侧重于分离植物化合物的提取、纯化和表征方法。本综述详细介绍了浸渍法、渗滤法、消化法、浸泡法、煎煮法、索氏蒸馏法和回流法、水蒸馏法和蒸汽蒸馏法等传统提取方法，以及 ASE、微波和超声波辅助提取、超临界流体提取、酶辅助流体提取、加压热水提取等现代提取技术。本综述还包括 LLF 和 LLP 的纯化方法、重结晶、不同的色谱技术，如 TLC、prep-TLC、柱色谱、闪速柱色谱、SEC、逆流色谱、HPLC 和 prep-GC。本综述还讨论了通过紫外可见光谱、傅立叶变换红外光谱、核磁共振光谱、质谱和 X 射线晶体学进行结构阐释的问题。

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来源期刊

Natural Product Research 化学-医药化学

CiteScore

5.10

自引率

9.10%

发文量

605

审稿时长

2.1 months

期刊介绍： The aim of Natural Product Research is to publish important contributions in the field of natural product chemistry. The journal covers all aspects of research in the chemistry and biochemistry of naturally occurring compounds. The communications include coverage of work on natural substances of land and sea and of plants, microbes and animals. Discussions of structure elucidation, synthesis and experimental biosynthesis of natural products as well as developments of methods in these areas are welcomed in the journal. Finally, research papers in fields on the chemistry-biology boundary, eg. fermentation chemistry, plant tissue culture investigations etc., are accepted into the journal. Natural Product Research issues will be subtitled either ""Part A - Synthesis and Structure"" or ""Part B - Bioactive Natural Products"". for details on this , see the forthcoming articles section. All manuscript submissions are subject to initial appraisal by the Editor, and, if found suitable for further consideration, to peer review by independent, anonymous expert referees. All peer review is single blind and submission is online via ScholarOne Manuscripts.