Cloning and Functional Characterization of Volatile-terpene Synthase Genes from Chamaecyparis obtusa var. formosana.

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Plant Science Pub Date : 2024-11-19 DOI:10.1016/j.plantsci.2024.112322

Ting-Wei Chen, Nai-Wen Tsao, Sheng-Yang Wang, Fang-Hua Chu

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Abstract

Chamaecyparis obtusa var. formosana is significant as a precious and endemic plant in Taiwan. The trunk, renowned for its excellent texture and color, is ideal for construction materials and furniture. Moreover, the entire plant is rich in aroma, which can be made into essential oils, fragrances, and a series of related products. Volatile terpenoids are the major compounds in the composition of essential oils, many of which can only be found in C. obtusa var. formosana. In this study, we successfully identified 12 volatile terpene synthases from C. obtusa var. formosana. Most of the selected TPSs displayed the ability to catalyze precursors into cyclic terpenoids, except for CovfTPS8, which also exhibited the capability to react with FPP and GPP. CovfTPS10 is particularly noteworthy for its multi-product characteristics and the ability to synthesize acoradiene. Moreover, it produces a novel compound, cis-isoduacene. Through the investigation of these volatile-terpenoid synthases, we can gain a better understanding of the cyclization process for terpenoids.

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Chamaecyparis obtusa var. formosana 的挥发性萜烯合成酶基因的克隆和功能特征。

Chamaecyparis obtusa var. formosana 是台湾特有的珍贵植物。其树干以质地和色泽优良而闻名，是建筑材料和家具的理想材料。此外，整株植物富含香气，可制成精油、香料和一系列相关产品。挥发性萜类化合物是精油成分中的主要化合物，其中许多只有在 C. obtusa var.在这项研究中，我们成功地从 C. obtusa var.除 CovfTPS8 外，大多数被选中的 TPSs 都具有将前体催化成环状萜类化合物的能力，CovfTPS8 还具有与 FPP 和 GPP 反应的能力。CovfTPS10 尤其值得注意的是它的多产品特性和合成炔丙二烯的能力。此外，它还能生成一种新型化合物--顺式异十二碳烯。通过对这些挥发性萜类化合物合成酶的研究，我们可以更好地了解萜类化合物的环化过程。

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来源期刊

Plant Science 生物-生化与分子生物学

CiteScore

9.10

自引率

1.90%

发文量

322

审稿时长

33 days

期刊介绍： Plant Science will publish in the minimum of time, research manuscripts as well as commissioned reviews and commentaries recommended by its referees in all areas of experimental plant biology with emphasis in the broad areas of genomics, proteomics, biochemistry (including enzymology), physiology, cell biology, development, genetics, functional plant breeding, systems biology and the interaction of plants with the environment. Manuscripts for full consideration should be written concisely and essentially as a final report. The main criterion for publication is that the manuscript must contain original and significant insights that lead to a better understanding of fundamental plant biology. Papers centering on plant cell culture should be of interest to a wide audience and methods employed result in a substantial improvement over existing established techniques and approaches. Methods papers are welcome only when the technique(s) described is novel or provides a major advancement of established protocols.