Antimicrobial efficacy of an experimental UV-C robot in controlled conditions and in a real hospital scenario

IF 3.9 3区医学 Q1 INFECTIOUS DISEASES Journal of Hospital Infection Pub Date : 2025-02-01 DOI:10.1016/j.jhin.2024.11.010

B. Casini , M. Scarpaci , F. Chiovelli , S. Leonetti , A.L. Costa , M. Baroni , M. Petrillo , F. Cavallo

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Abstract

Background

Among no-touch automated disinfection devices, ultraviolet-C (UV-C) radiation has been proven to be one of the most effective against a broad spectrum of micro-organisms causing healthcare-associated infections.

Aim

To evaluate the antimicrobial efficacy of an experimental UV-C robotic platform, under controlled conditions and in a real hospital scenario, when used to implement the standard cleaning operating protocol (SOP).

Methods

In vitro, following dose calibration tests, bactericidal and virucidal efficacy were tested in accordance with American Society for Testing and Materials International Standard E3135-18. In hospital settings, 12 high-touch surfaces were sampled after healthcare activity (dirty condition), after SOP alone, and after SOP + UV-C treatment, with a total of 180 samples.

Findings

In vitro, <4 mJ/cm² was required to remove Staphylococcus aureus and Pseudomonas aeruginosa completely, 194 mJ/cm² was required to inactivate adenovirus HadV5 completely, and 38.8 mJ/cm² was sufficient to inactivate coronavirus 229E completely. In the real hospital scenario, the mean UV-C dose emitted on the sampled surfaces was 29.31 mJ/cm². A significant difference was found after SOP alone (P=0.022) and after SOP + UV-C treatment (P=0.007) compared with the dirty condition. The average percentage reduction in the total viable count (TVC) was 67% after SOP alone and 96% after SOP + UV-C treatment.

Conclusions

Comparison of the tests conducted in vitro and in the real hospital scenario showed that the efficacy of the UV-C robot was reduced in the hospital setting, as a higher dose was needed to obtain a reduction in the TVC.

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在受控条件下和真实医院场景中，实验性紫外线-C 机器人的抗菌功效。

背景：目的：评估实验性紫外线-C 机器人平台在受控条件下和实际医院场景中执行标准清洁操作规程（SOP）时的抗菌效果：方法：根据 ASTM E3135-18 标准，在体外进行剂量校准测试后，测试杀菌和杀病毒效果。在医院环境中，对医疗活动后、SOP 或 SOP 和紫外线-C 处理后的 12 个高接触表面进行了取样，共计 180 个样本：在体外，彻底清除金黄色葡萄球菌和绿脓杆菌所需的剂量小于 4 mJ/cm2，彻底灭活腺病毒 HadV5 所需的剂量为 194 mJ/cm2，而彻底灭活冠状病毒 229E 所需的剂量为 38.8 mJ/cm2。在真实场景中，采样表面发射的紫外线-C 剂量平均为 29.31 mJ/cm2。我们发现，经过 SOP 处理（p 值 = 0.022）和紫外线-C 处理（p 值 = 0.007）后获得的数据与脏污条件下获得的数据相比有明显差异。经过 SOP 处理后，总存活计数（TVC）平均减少了 67%，而经过 SOP 和紫外线-C 联合处理后，总存活计数平均减少了 96%：通过比较体外测试和实际场景中的测试，我们发现在医院环境中，紫外线-C 机器人的功效有所降低，因为与体外测试不同的是，必须使用比体外测试更高的剂量，才能使总存活数（TVC）减少，但总存活数甚至没有减少。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Hospital Infection 医学-传染病学

CiteScore

12.70

自引率

5.80%

发文量

271

审稿时长

19 days

期刊介绍： The Journal of Hospital Infection is the editorially independent scientific publication of the Healthcare Infection Society. The aim of the Journal is to publish high quality research and information relating to infection prevention and control that is relevant to an international audience. The Journal welcomes submissions that relate to all aspects of infection prevention and control in healthcare settings. This includes submissions that: provide new insight into the epidemiology, surveillance, or prevention and control of healthcare-associated infections and antimicrobial resistance in healthcare settings; provide new insight into cleaning, disinfection and decontamination; provide new insight into the design of healthcare premises; describe novel aspects of outbreaks of infection; throw light on techniques for effective antimicrobial stewardship; describe novel techniques (laboratory-based or point of care) for the detection of infection or antimicrobial resistance in the healthcare setting, particularly if these can be used to facilitate infection prevention and control; improve understanding of the motivations of safe healthcare behaviour, or describe techniques for achieving behavioural and cultural change; improve understanding of the use of IT systems in infection surveillance and prevention and control.