{"title":"Lead Nitrate (Pb(NO<sub>3</sub>)<sub>2</sub>) Toxicity Effects on DNA Structure and Histopathological Damage in Gills of Common Carp (Cyprinus carpio).","authors":"Ritu Sharma, Rajinder Jindal, Nikhil Jhamb, Mahdi Banaee, Caterina Faggio","doi":"10.1002/jemt.24748","DOIUrl":null,"url":null,"abstract":"<p><p>The toxic impact of environmentally relevant nominal sub-lethal concentration of lead nitrate (23 mg/L) on the gills of Cyprinus carpio after 30 days of exposure was assessed. Structural alterations were analyzed through histopathology, and the DNA damage rate in peripheral erythrocytes was evaluated by alkaline comet assay. A significant deviation in the gill histoarchitecture was observed compared to the control group. Significant changes, including the curling of secondary lamellae, loss of filaments, necrosis, hyperplasia, hypertrophy of cells of primary filament, and vacuolization, were found in the fish exposed to nominal sub-lethal concentration of lead nitrate. Moreover, the comet assay showed lead nitrate-induced DNA damage, evidenced by the length of the DNA \"tail\" in the exposed fish. The findings of this study strongly indicated that lead nitrate, even at sub-lethal levels, could significantly alter the overall physiology of the fish. This situation could lead to severe ecological consequences.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0000,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microscopy Research and Technique","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1002/jemt.24748","RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ANATOMY & MORPHOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
The toxic impact of environmentally relevant nominal sub-lethal concentration of lead nitrate (23 mg/L) on the gills of Cyprinus carpio after 30 days of exposure was assessed. Structural alterations were analyzed through histopathology, and the DNA damage rate in peripheral erythrocytes was evaluated by alkaline comet assay. A significant deviation in the gill histoarchitecture was observed compared to the control group. Significant changes, including the curling of secondary lamellae, loss of filaments, necrosis, hyperplasia, hypertrophy of cells of primary filament, and vacuolization, were found in the fish exposed to nominal sub-lethal concentration of lead nitrate. Moreover, the comet assay showed lead nitrate-induced DNA damage, evidenced by the length of the DNA "tail" in the exposed fish. The findings of this study strongly indicated that lead nitrate, even at sub-lethal levels, could significantly alter the overall physiology of the fish. This situation could lead to severe ecological consequences.
评估了与环境相关的亚致死浓度硝酸铅（23 毫克/升）在接触鲤鱼鳃 30 天后对其产生的毒性影响。通过组织病理学分析了鳃的结构变化，并通过碱性彗星试验评估了外周血红细胞的 DNA 损伤率。与对照组相比，观察到鳃组织结构发生了明显偏差。在标称亚致死浓度的硝酸铅暴露下，鱼鳃发生了显著变化，包括次生薄片卷曲、细丝脱落、坏死、增生、初级细丝细胞肥大和空泡化。此外，彗星试验显示，硝酸铅会诱发 DNA 损伤，暴露鱼类 DNA "尾巴 "的长度就是证明。这项研究的结果有力地表明，即使是亚致死浓度的硝酸铅，也会显著改变鱼类的整体生理机能。这种情况可能会导致严重的生态后果。
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Microscopy Research and Technique (MRT) publishes articles on all aspects of advanced microscopy original architecture and methodologies with applications in the biological, clinical, chemical, and materials sciences. Original basic and applied research as well as technical papers dealing with the various subsets of microscopy are encouraged. MRT is the right form for those developing new microscopy methods or using the microscope to answer key questions in basic and applied research.
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