Genome-wide association study identified BnaPAP17 genes involved in exogenous ATP utilization and regulating phosphorous content in Brassica napus.

IF 5.3 2区 生物学 Q1 PLANT SCIENCES Plant Cell Reports Pub Date : 2024-11-25 DOI:10.1007/s00299-024-03373-x
Hao Li, Haijiang Liu, Chuang Wang, Yang Zeng, Surya Kant, Xiaohua Wang, John P Hammond, Guangda Ding, Hongmei Cai, Sheliang Wang, Fangsen Xu, Ying Zhang, Lei Shi
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Abstract

Key message: BnaPAP17s associated with root-secreted APases activity were identified by genome-wide association study, and those were induced by Pi-deficiency. BnaPAP17s were involved in improving exogenous organophosphorus utilization as secreted APases. Deficiency of available phosphorus (P) in soil has become an important limiting factor for yield and quality in oilseed rape (Brassica napus). In many soils, organic P (Po) is the main component of the soil P pool. Po must be hydrolyzed to inorganic P (Pi) through acid Phosphatase (APases), and then taken up by plants. However, root-secreted APases (SAP) activity, as a quantitative trait, plays an important role in soil Po utilization; those genetic loci are not clear in B. napus. In this study, we performed a genome-wide association study for SAP activity under Pi-deficiency using a panel of 350 accessions of B. napus and more than 4.5 million polymorphic single nucleotide polymorphisms (SNPs). Thirty-five significant SNPs associated with SAP activity were identified. BnaA01.PAP17 (BnaA01g27810D) was a candidate gene underlying lead SNP (ChrA01_19576615). We experimentally verified that both BnaA01.PAP17 and its three homologous genes had similar expression pattern in response to Pi-deficiency. The dynamic changes in BnaPAP17s expression level were opposite to those of Pi concentration in both roots and leaves, suggesting their potential utility as Pi marker genes in B. napus. Transient expression of BnaPAP17s in tobacco leaves proved that BnaPAP17s were located in the apoplast as secreted APases. The overexpression of BnaPAP17s enhanced SAP activity in response to Pi-deficiency and resulting in increased P content in plants when ATP was supplied as the sole P resource. Taken together, these results suggest that BnaPAP17s contributed to SAP activity, thus having a function in extracellular Po utilization in B. napus.

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全基因组关联研究发现了参与外源 ATP 利用和调节甘蓝型油菜磷含量的 BnaPAP17 基因。
关键信息通过全基因组关联研究发现了与根分泌型AP酶活性相关的BnaPAP17s,它们是由Pi-缺失诱导的。BnaPAP17s作为分泌型AP酶参与提高外源有机磷的利用率。土壤中可用磷(P)的缺乏已成为油菜(Brassica napus)产量和质量的重要限制因素。在许多土壤中,有机磷(Po)是土壤磷库的主要成分。Po 必须通过酸性磷酸酶(APase)水解为无机 P(Pi),然后被植物吸收。然而,根分泌的磷酸酶(SAP)活性作为一种数量性状,在土壤Po的利用中发挥着重要作用;而这些遗传位点在油菜中并不明确。在这项研究中,我们利用350个番茄品种和450多万个多态单核苷酸多态性(SNPs),对Pi-缺失条件下的SAP活性进行了全基因组关联研究。结果发现了 35 个与 SAP 活性相关的重要 SNPs。BnaA01.PAP17(BnaA01g27810D)是一个潜在的候选基因,它是主导 SNP(ChrA01_19576615)的基础。我们通过实验验证了 BnaA01.PAP17 及其三个同源基因在 Pi- 缺乏时具有相似的表达模式。BnaPAP17s 表达水平的动态变化与根部和叶片中 Pi 浓度的变化相反,这表明它们有可能成为油菜中的 Pi 标记基因。BnaPAP17s 在烟草叶片中的瞬时表达证明,BnaPAP17s 作为分泌型 AP 酶位于细胞质中。BnaPAP17s 的过表达增强了 SAP 在 Pi- 缺乏时的活性,当 ATP 作为唯一的 P 资源被提供时,植物体内的 P 含量也随之增加。综上所述,这些结果表明 BnaPAP17s 促进了 SAP 的活性,因此在油菜中具有细胞外 Po 利用的功能。
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来源期刊
Plant Cell Reports
Plant Cell Reports 生物-植物科学
CiteScore
10.80
自引率
1.60%
发文量
135
审稿时长
3.2 months
期刊介绍: Plant Cell Reports publishes original, peer-reviewed articles on new advances in all aspects of plant cell science, plant genetics and molecular biology. Papers selected for publication contribute significant new advances to clearly identified technological problems and/or biological questions. The articles will prove relevant beyond the narrow topic of interest to a readership with broad scientific background. The coverage includes such topics as: - genomics and genetics - metabolism - cell biology - abiotic and biotic stress - phytopathology - gene transfer and expression - molecular pharming - systems biology - nanobiotechnology - genome editing - phenomics and synthetic biology The journal also publishes opinion papers, review and focus articles on the latest developments and new advances in research and technology in plant molecular biology and biotechnology.
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