{"title":"Comparative evaluation of sealing ability and adaptation of gel form of MTA to dentinal walls: an in-vitro study.","authors":"Haritha Parthasarathy, Akshara Modak, Karuna Yarmunja Mahabala, Srikant Natarajan, Shrikala Baliga, Manuel Thomas, Ashwin Rao, Anupama Nayak","doi":"10.1038/s41405-024-00271-y","DOIUrl":null,"url":null,"abstract":"<p><strong>Context: </strong>Mineral Trioxide Aggregate (MTA) is a calcium silicate-based cement that potentially exhibits improved washout resistance when carboxymethyl chitosan or gelatin is incorporated. Gel-form MTA is a novel mineral trioxide aggregate formulated using construction industry-based technology. The present study was conducted to comparatively evaluate the sealing ability and adaptation to dentinal walls of gel-form MTA.</p><p><strong>Materials and methods: </strong>This in-vitro study consisted of two groups: gel-form MTA and the conventional powder-liquid MTA. 10 samples per group were used for each of the tested parameters. Adaptation of the MTA to the dentinal walls was tested under the light microscope and measured using Image J software. Sealing ability was evaluated using a single aerobic bacterial leakage model. Appropriate statistical analysis was done for the obtained data. Adaptation of the MTA was analyzed using independent t-test and Friedman test, whereas the bacterial leakage was analyzed using chi-square test.</p><p><strong>Results: </strong>On comparison of the adaptation property at coronal and apical thirds, there was no statistically significant difference between the groups (p = 0.071 and p = 0.638, respectively). However, while comparing the same in the middle one-third of the root, lesser gaps were identified in the gel-form MTA group (p = 0.013). One sample belonging to the conventional powder-liquid MTA group showed significant turbidity during bacteria leakage evaluation (p = 0.001) with the presence of E. faecalis in the count of 10<sup>3</sup> colony forming units/milliliter.</p><p><strong>Conclusion: </strong>The gel-form MTA shows a better adaptation to the dentinal walls at the middle third of the root and exhibits better sealing ability against bacterial leakage when tested for E. faecalis. The adaptation of gel-form MTA at coronal and apical third of the root was comparable to the conventional powder-liquid MTA.</p>","PeriodicalId":36997,"journal":{"name":"BDJ Open","volume":"10 1","pages":"86"},"PeriodicalIF":2.5000,"publicationDate":"2024-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11586422/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"BDJ Open","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1038/s41405-024-00271-y","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
引用次数: 0
Abstract
Context: Mineral Trioxide Aggregate (MTA) is a calcium silicate-based cement that potentially exhibits improved washout resistance when carboxymethyl chitosan or gelatin is incorporated. Gel-form MTA is a novel mineral trioxide aggregate formulated using construction industry-based technology. The present study was conducted to comparatively evaluate the sealing ability and adaptation to dentinal walls of gel-form MTA.
Materials and methods: This in-vitro study consisted of two groups: gel-form MTA and the conventional powder-liquid MTA. 10 samples per group were used for each of the tested parameters. Adaptation of the MTA to the dentinal walls was tested under the light microscope and measured using Image J software. Sealing ability was evaluated using a single aerobic bacterial leakage model. Appropriate statistical analysis was done for the obtained data. Adaptation of the MTA was analyzed using independent t-test and Friedman test, whereas the bacterial leakage was analyzed using chi-square test.
Results: On comparison of the adaptation property at coronal and apical thirds, there was no statistically significant difference between the groups (p = 0.071 and p = 0.638, respectively). However, while comparing the same in the middle one-third of the root, lesser gaps were identified in the gel-form MTA group (p = 0.013). One sample belonging to the conventional powder-liquid MTA group showed significant turbidity during bacteria leakage evaluation (p = 0.001) with the presence of E. faecalis in the count of 103 colony forming units/milliliter.
Conclusion: The gel-form MTA shows a better adaptation to the dentinal walls at the middle third of the root and exhibits better sealing ability against bacterial leakage when tested for E. faecalis. The adaptation of gel-form MTA at coronal and apical third of the root was comparable to the conventional powder-liquid MTA.
背景:三氧化物矿物质骨料(MTA)是一种硅酸钙基水泥,在掺入羧甲基壳聚糖或明胶后,其抗冲刷性可能会得到改善。凝胶状 MTA 是一种新型的三氧化二铝矿物骨料,采用建筑行业的技术配制而成。本研究旨在比较评估凝胶状 MTA 的密封能力和对牙本质壁的适应性:这项体外研究包括两组:凝胶状 MTA 和传统的粉液 MTA。每组 10 个样本,每个样本用于测试各项参数。在光学显微镜下测试 MTA 对牙本质壁的适应性,并使用 Image J 软件进行测量。密封能力采用单一需氧细菌渗漏模型进行评估。对获得的数据进行了适当的统计分析。MTA 的适应性采用独立 t 检验和 Friedman 检验进行分析,而细菌渗漏则采用卡方检验进行分析:结果:比较冠状面和根尖三分之一处的适应性,两组之间没有明显的统计学差异(分别为 p = 0.071 和 p = 0.638)。然而,在比较牙根中间三分之一处的适应性时,发现凝胶形态 MTA 组的间隙较小(p = 0.013)。在细菌渗漏评估过程中,属于传统粉末-液体 MTA 组的一个样本出现了明显的浑浊(p = 0.001),其中粪大肠杆菌的菌落形成单位为 103 个/毫升:结论:凝胶状 MTA 对牙根中三分之一处的牙本质壁有更好的适应性,在粪大肠杆菌检测中显示出更好的密封能力,防止细菌渗漏。凝胶状 MTA 在牙根冠部和根尖三分之一处的适应性与传统的粉末液体 MTA 相当。