Novel Prognostic Markers for Skin Cutaneous Melanoma.

IF 1.9 4区医学 Q3 DERMATOLOGY Clinical, Cosmetic and Investigational Dermatology Pub Date : 2024-11-20 eCollection Date: 2024-01-01 DOI:10.2147/CCID.S486679

Yi Zhang, Ansheng Xie, Di Wang, Weiwei Deng

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Abstract

Background: Skin cutaneous melanoma (SKCM) ranks among the most prevalent malignant tumors, highlighting the significance of identifying new research targets. In this study, our objective was to pinpoint pivotal genes implicated in SKCM pathogenesis and ascertain their potential as prognostic biomarkers.

Methods: Leveraging data from 1809 normal skin samples and 471 SKCM samples, we identified differentially expressed genes (DEGs). Using a comprehensive suite of bioinformatic analyses, including weighted gene co-expression network analysis (WGCNA), we elucidated the functions of these DEGs and singled out hub genes. Cox analyses and overall survival analyses underscored that elevated expression of these genes correlated with more favorable prognoses.

Results: Ultimately, we identified five genes (PLAC8, IL4I1, ZNF80, CCR8, CLEC4C) as novel prognostic markers for SKCM. Furthermore, multivariate Cox analyses pinpointed ZNF80 and CCR8 as independent prognostic biomarkers. Experimental validation targeting these genes revealed significant downregulation in melanoma cells, except for CCR8. Subsequent knockdown of IL4I1 promoted both the proliferation and inhibited the apoptosis of melanoma cells.

Conclusion: In summary, our study identified a series of potential prognostic genes in melanoma and verified the functional role of IL4I1 among them.

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皮肤黑色素瘤的新型预后标志物

背景：皮肤黑色素瘤（SKCM）是发病率最高的恶性肿瘤之一，因此确定新的研究目标意义重大。在这项研究中，我们的目标是找出与 SKCM 发病机制有关的关键基因，并确定它们作为预后生物标志物的潜力：方法：利用来自 1809 份正常皮肤样本和 471 份 SKCM 样本的数据，我们确定了差异表达基因（DEGs）。利用一套全面的生物信息学分析，包括加权基因共表达网络分析（WGCNA），我们阐明了这些差异表达基因的功能，并挑出了枢纽基因。Cox分析和总生存分析显示，这些基因的高表达与更有利的预后相关：结果：我们最终确定了五个基因（PLAC8、IL4I1、ZNF80、CCR8、CLEC4C）作为 SKCM 的新型预后标记。此外，多变量考克斯分析将 ZNF80 和 CCR8 确定为独立的预后生物标志物。针对这些基因的实验验证显示，除 CCR8 外，其他基因在黑色素瘤细胞中的表达均显著下调。随后，IL4I1的敲除既促进了黑色素瘤细胞的增殖，又抑制了其凋亡：总之，我们的研究发现了一系列黑色素瘤潜在的预后基因，并验证了其中IL4I1的功能作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical, Cosmetic and Investigational Dermatology Medicine-Dermatology

CiteScore

2.80

自引率

4.30%

发文量

353

审稿时长

16 weeks

期刊介绍： Clinical, Cosmetic and Investigational Dermatology is an international, peer-reviewed, open access journal that focuses on the latest clinical and experimental research in all aspects of skin disease and cosmetic interventions. Normal and pathological processes in skin development and aging, their modification and treatment, as well as basic research into histology of dermal and dermal structures that provide clinical insights and potential treatment options are key topics for the journal. Patient satisfaction, preference, quality of life, compliance, persistence and their role in developing new management options to optimize outcomes for target conditions constitute major areas of interest. The journal is characterized by the rapid reporting of clinical studies, reviews and original research in skin research and skin care. All areas of dermatology will be covered; contributions will be welcomed from all clinicians and basic science researchers globally.