Role of Dalbergia sissoo as host species in physiological and molecular adaptation of sandalwood under individual and interactive salinity and water deficit stress

IF 6.8 Q1 PLANT SCIENCES Plant Stress Pub Date : 2024-11-19 DOI:10.1016/j.stress.2024.100679
Aarju Sharma , Ashwani Kumar , Sulekha Chahal , Pooja Dhansu , Raj Kumar , Shruti Kaushik , Bindu Battan , Parvender Sheoran , Poonam Choudhary
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Abstract

The aim of present study was to investigate the viability of cultivating white sandalwood (Santalum album L.) in sub-tropical India where farmers are primarily concerned with salinity stress and water deficit as well as different genes that regulate the growth. The present research was undertaken to explore the molecular mechanism of salinity and drought tolerance in sandalwood planted with Dalbergia sissoo (selected based on prior studies) by conducting an RBD experiment under water deficit (50 %), salinity stress (ECiw ∼ 8 ds/m) and combined 50 % water deficit and saline stress (ECiw ∼ 8 ds/m). After two years of imposed treatments, leaves were collected from sandalwood to study the relative gene expression of salinity tolerance (SOS 1, NHX 1 and NHX 2), antioxidant enzymes (SOD, CAT, APX and POX), proline synthesis (P5CS and P5CR) and nitrogen metabolism (NR, NIR, GS and GDH) related genes using real-time PCR (RT-PCR). Different morpho-physiological and biochemical traits showed reduction under individual and interactive stresses except proline and Na+ content as well as anti-oxidative enzyme activities. So far, gene expression studies have not been fully validated in sandalwood under abiotic stresses. The results displayed that SOS 1, NHX 1, NHX 2, APX, POX, CAT, SOD, P5CS and P5CR genes showed maximum expression under combined salinity and water deficit stress. On the other side, genes involved in nitrogen metabolism, i.e., NR, NIR, GS and GDH showed lowest expression under individual as well as interactive water deficit and salinity stress. The current study also highlights the significance of the host D. sissoo which may be good long-term host species in terms of stress tolerance mechanism at molecular level for sandalwood production under changing environmental conditions.
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Dalbergia sissoo 作为宿主物种在单独和交互盐度及缺水胁迫下对檀香生理和分子适应性的作用
本研究的目的是调查在印度亚热带地区种植白檀(Santalum album L.)的可行性,那里的农民主要关注盐度胁迫和水分亏缺以及调节生长的不同基因。本研究通过在缺水(50%)、盐分胁迫(ECiw ∼ 8 ds/m)以及 50%缺水和盐分胁迫(ECiw ∼ 8 ds/m)条件下进行 RBD 试验,探索了白檀(Dalbergia sissoo,根据先前研究选定)耐盐碱和耐干旱的分子机制。经过两年的处理后,采集檀香叶片,利用实时 PCR(RT-PCR)技术研究耐盐基因(SOS 1、NHX 1 和 NHX 2)、抗氧化酶(SOD、CAT、APX 和 POX)、脯氨酸合成基因(P5CS 和 P5CR)以及氮代谢相关基因(NR、NIR、GS 和 GDH)的相对表达。除脯氨酸和 Na+ 含量以及抗氧化酶活性外,不同的形态生理和生化性状在单独胁迫和交互胁迫下均有所下降。迄今为止,非生物胁迫下的檀香基因表达研究尚未得到充分验证。研究结果表明,在盐度和缺水联合胁迫下,SOS 1、NHX 1、NHX 2、APX、POX、CAT、SOD、P5CS 和 P5CR 基因的表达量最大。另一方面,参与氮代谢的基因,即 NR、NIR、GS 和 GDH 在单独以及缺水和盐度胁迫交互作用下的表达量最低。本研究还强调了宿主 D. sissoo 的重要性,它可能是在不断变化的环境条件下,檀香生产在分子水平上具有良好抗逆机制的长期宿主物种。
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来源期刊
Plant Stress
Plant Stress PLANT SCIENCES-
CiteScore
5.20
自引率
8.00%
发文量
76
审稿时长
63 days
期刊介绍: The journal Plant Stress deals with plant (or other photoautotrophs, such as algae, cyanobacteria and lichens) responses to abiotic and biotic stress factors that can result in limited growth and productivity. Such responses can be analyzed and described at a physiological, biochemical and molecular level. Experimental approaches/technologies aiming to improve growth and productivity with a potential for downstream validation under stress conditions will also be considered. Both fundamental and applied research manuscripts are welcome, provided that clear mechanistic hypotheses are made and descriptive approaches are avoided. In addition, high-quality review articles will also be considered, provided they follow a critical approach and stimulate thought for future research avenues. Plant Stress welcomes high-quality manuscripts related (but not limited) to interactions between plants and: Lack of water (drought) and excess (flooding), Salinity stress, Elevated temperature and/or low temperature (chilling and freezing), Hypoxia and/or anoxia, Mineral nutrient excess and/or deficiency, Heavy metals and/or metalloids, Plant priming (chemical, biological, physiological, nanomaterial, biostimulant) approaches for improved stress protection, Viral, phytoplasma, bacterial and fungal plant-pathogen interactions. The journal welcomes basic and applied research articles, as well as review articles and short communications. All submitted manuscripts will be subject to a thorough peer-reviewing process.
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