Isolation and characterization of Salmonella enterica- and Escherichia coli-specific bacteriophages of the genus Epseptimavirus from wastewater in Minnesota
Estephany Cortes-Ortega, Eleanore G. Hansen, Irem Iskender, Meredith L. Farmer, Juan M. Martinez-Villalobos, Jacob D. Vitt, Steven D. Bowden
{"title":"Isolation and characterization of Salmonella enterica- and Escherichia coli-specific bacteriophages of the genus Epseptimavirus from wastewater in Minnesota","authors":"Estephany Cortes-Ortega, Eleanore G. Hansen, Irem Iskender, Meredith L. Farmer, Juan M. Martinez-Villalobos, Jacob D. Vitt, Steven D. Bowden","doi":"10.1007/s00705-024-06190-5","DOIUrl":null,"url":null,"abstract":"<div><p>Five lytic bacteriophages specific for <i>Salmonella enterica</i> and <i>Escherichia coli</i> were isolated from wastewater in Minnesota. These phages, designated vB_Sal_EH1, vB_Sal_EH2, vB_Sal_EH3, vB_Sal_EH4, and vB_Sal_EH7, were characterized, and their genomes were sequenced. Phylogenetic analysis showed that they grouped within the genus <i>Epseptimavirus</i>, with genome sizes ranging from 108,554 to 115,218 bp. All five phages exhibited lytic activity against both <i>S</i>. <i>enterica</i> and Shiga-toxin-producing <i>E. coli</i> O157:H7. Transposon mutagenesis of the host genome identified the outer membrane protein BtuB as essential for phage infection, suggesting that it is a putative receptor. Genome sequence comparisons revealed genetic loci that are variable among the isolated phages and potentially influence their host specificity and virulence.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"169 12","pages":""},"PeriodicalIF":2.5000,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Archives of Virology","FirstCategoryId":"3","ListUrlMain":"https://link.springer.com/article/10.1007/s00705-024-06190-5","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"VIROLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Five lytic bacteriophages specific for Salmonella enterica and Escherichia coli were isolated from wastewater in Minnesota. These phages, designated vB_Sal_EH1, vB_Sal_EH2, vB_Sal_EH3, vB_Sal_EH4, and vB_Sal_EH7, were characterized, and their genomes were sequenced. Phylogenetic analysis showed that they grouped within the genus Epseptimavirus, with genome sizes ranging from 108,554 to 115,218 bp. All five phages exhibited lytic activity against both S. enterica and Shiga-toxin-producing E. coli O157:H7. Transposon mutagenesis of the host genome identified the outer membrane protein BtuB as essential for phage infection, suggesting that it is a putative receptor. Genome sequence comparisons revealed genetic loci that are variable among the isolated phages and potentially influence their host specificity and virulence.
期刊介绍：
Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
