Molecular detection of Leishmania DNA in wild-caught sand flies, Phlebotomus and Sergentomyia spp. in northern Iran

IF 2 Q3 INFECTIOUS DISEASES Parasite Epidemiology and Control Pub Date : 2024-11-01 DOI:10.1016/j.parepi.2024.e00395

Seyed Hassan Nikookar , Mohammad Reza Akbari , Mohammad Ali Oshaghi , Nasibeh Hosseini-Vasoukolaei , Ahmadali Enayati , Farzad Motevalli-Haghi , Mahdi Fakhar

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Abstract

Leishmaniasis is currently considered a major health problem in Iran, posing an increasing threat to society's development in various dimensions. This study aimed to detect Leishmania infection in wild-caught sand flies in Sari City, northern Iran. Sand flies were collected using sticky traps, and Leishmania DNA was identified using polymerase chain reaction (PCR) targeting the ITS2-rDNA region, followed by restriction fragment length polymorphism (RFLP) analysis. A total of 138 female sand flies were tested, among which, only 1 specimen of Ph. papatasi (11.11 %) and Ph. major (14.28 %), 4 specimens of Ph. kandelakii (7.27 %) and Se. dentata (8.33 %), and 2 specimens of Se. sintoni (50 %) were naturally infected with L. (L.) major. This was observed in the ITS2 nested-PCR amplification assays where a ∼ 245 bp PCR band was produced. Also, RFLP analysis by Mnl1 revealed the fragments of 55 and 70 and 120 bp for infected sand flies which are characteristic of L. (L.) major. Most of the sand flies were unfed, collected during warm season, found indoor. This study reperesents the first molecular detection of L. (L.) major in wild-caught sand flies, specifically in Ph. papatasi in this region, as well as Ph. kandelakii and Ph. major in Iran and even the world.

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伊朗北部野生沙蝇、白蛉和瑟gentomyia spj中利什曼原虫DNA的分子检测

利什曼病目前被认为是伊朗的一个主要健康问题，在各个方面对社会发展构成越来越大的威胁。本研究旨在检测伊朗北部萨里市野生捕获的沙蝇感染利什曼原虫。采用粘捕法采集沙蝇，利用针对ITS2-rDNA区域的聚合酶链反应（PCR）鉴定利什曼原虫DNA，并进行限制性片段长度多态性（RFLP）分析。共检测雌沙蝇138只，其中帕帕塔西白僵菌1只（11.11%），大黄僵菌1只（14.28%），坎德拉基白僵菌4只（7.27%），大黄僵菌4只（7.27%）。齿状体（8.33%）；sintoni（50%）自然感染L. (L.) major。这在ITS2巢式PCR扩增实验中观察到，产生了一个~ 245 bp的PCR条带。Mnl1的RFLP分析显示，感染沙蝇的55、70和120 bp的片段具有L. (L.) major的特征。大多数沙蝇都是在温暖的季节收集的，在室内发现的。本研究首次在野外捕获的沙蝇中检测到L. (L.) major，特别是在该地区的Ph. papatasi，以及伊朗乃至世界范围内的Ph. kandelakii和Ph. major。

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来源期刊

Parasite Epidemiology and Control Medicine-Infectious Diseases

CiteScore

5.70

自引率

3.10%

发文量

审稿时长

17 weeks

期刊介绍： Parasite Epidemiology and Control is an Open Access journal. There is an increasing amount of research in the parasitology area that analyses the patterns, causes, and effects of health and disease conditions in defined populations. This epidemiology of parasite infectious diseases is predominantly studied in human populations but also spans other major hosts of parasitic infections and as such this journal will have a broad remit. We will focus on the major areas of epidemiological study including disease etiology, disease surveillance, drug resistance and geographical spread and screening, biomonitoring, and comparisons of treatment effects in clinical trials for both human and other animals. We will also look at the epidemiology and control of vector insects. The journal will also cover the use of geographic information systems (Epi-GIS) for epidemiological surveillance which is a rapidly growing area of research in infectious diseases. Molecular epidemiological approaches are also particularly encouraged.