Fast phase distortion identification and automatic distortion compensated reconstruction for digital holographic microscopy using deep learning

IF 3.7 2区 工程技术 Q2 OPTICS Optics and Lasers in Engineering Pub Date : 2025-02-01 Epub Date: 2024-11-26 DOI:10.1016/j.optlaseng.2024.108718
Zihan Lin, Shuhai Jia, YuanCheng Xu, Bo Wen, Huajian Zhang, Longning Wang, Mengyu Han
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Abstract

Digital holographic microscopy (DHM) is a quantitative phase measurement technique with full-field, contactless, and fast. The technique provides accurate micro-surface morphology of samples. These steps are essential for accurate phase reconstruction, such as holographic focusing, numerical diffraction, phase unwrapping and distortion compensation. Performing these processes manually is time-consuming and is not conducive to the general application of the technology. In order to improve the detection efficiency, this paper proposes a deep learning model that can achieve fast identification of DHM phase distortion and automatic phase distortion compensation reconstruction. The model can be preprocessed for holographic phase to accurately identify the type of phase distortion present in the phase. And adaptively adjust the network weight parameters for phase distortion compensation reconstruction. The experimental results show that the method proposed in this paper achieves fast and accurate identification of multiple phase distortions. The model has high accuracy and strong generalization ability. The reconstructed holographic phase map has PSNR of 35.2743dB and RMSE as low as 10-2 level in the face of complex mixed aberrations. The identification and reconstruction processes took 0.005s and 0.058s, both in milliseconds, respectively. The evaluation indexes SSIM, FSIM and NC can reach above 0.99. It is shown that the method in this paper is not only capable of reconstructing holograms, but also able to effectively retain the detailed features of the original image.
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基于深度学习的数字全息显微镜相位畸变快速识别与自动畸变补偿重建
数字全息显微镜(DHM)是一种全视野、无接触、快速的相位定量测量技术。该技术提供了精确的样品微表面形貌。这些步骤对于精确的相位重建至关重要,如全息聚焦、数值衍射、相位展开和畸变补偿。手动执行这些过程非常耗时,并且不利于该技术的一般应用。为了提高检测效率,本文提出了一种能够快速识别DHM相位畸变并实现相位畸变自动补偿重建的深度学习模型。该模型可以对全息相位进行预处理,以准确识别相位中存在的相位畸变类型。并自适应调整网络权值参数进行相位畸变补偿重建。实验结果表明,本文提出的方法能够快速准确地识别多相位畸变。该模型精度高,泛化能力强。在复杂混合像差情况下,重建全息相位图的PSNR为35.2743dB, RMSE低至10-2级。识别和重建过程分别耗时0.005s和0.058s,均以毫秒为单位。评价指标SSIM、FSIM和NC均达到0.99以上。实验结果表明,本文方法不仅能够重建全息图,而且能够有效地保留原始图像的细节特征。
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来源期刊
Optics and Lasers in Engineering
Optics and Lasers in Engineering 工程技术-光学
CiteScore
8.90
自引率
8.70%
发文量
384
审稿时长
42 days
期刊介绍: Optics and Lasers in Engineering aims at providing an international forum for the interchange of information on the development of optical techniques and laser technology in engineering. Emphasis is placed on contributions targeted at the practical use of methods and devices, the development and enhancement of solutions and new theoretical concepts for experimental methods. Optics and Lasers in Engineering reflects the main areas in which optical methods are being used and developed for an engineering environment. Manuscripts should offer clear evidence of novelty and significance. Papers focusing on parameter optimization or computational issues are not suitable. Similarly, papers focussed on an application rather than the optical method fall outside the journal''s scope. The scope of the journal is defined to include the following: -Optical Metrology- Optical Methods for 3D visualization and virtual engineering- Optical Techniques for Microsystems- Imaging, Microscopy and Adaptive Optics- Computational Imaging- Laser methods in manufacturing- Integrated optical and photonic sensors- Optics and Photonics in Life Science- Hyperspectral and spectroscopic methods- Infrared and Terahertz techniques
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