Morphology of Pseudomonas aeruginosa typing phages of the lindberg set

H.-W. Ackermann , C. Cartier , S. Ślopek , J.-F. Vieu
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引用次数: 36

Abstract

Eighteen phages were studied by electron microscopy. They belonged to eight morphotypes representing the Myoviridae, Siphoviridae and Podoviridae families of tailed phages. Twelve phages were members of known species and six phages belonged to three new species. Results correlated closely with serological and DNA-DNA hybridization data. Induced and non-induced propagating bacteria were investigated for lysogeny. All strains produced R-type pyocins, phage-like particles or inhibitory reactions. The identity of typing phages should be controlled periodically.

Dix-huit phages sont étudiés au microscope électronique. Ils sont caudés, appartiennent à huit types morphologiques et représentent les familles des Myo-, Sipho-, et Podoviridae. Douze phages appartiennent à des espèces connues et six phages, à trois espèces nouvelles. Il y a une étroite corrélation entre la morphologie, la sérologie et l'hybridation de l'ADN des phages. L'état lysogène des bactéries propagatrices est étudié avant et après induction. Toutes les souches produisent des pyocines du type R ou des particules phagiques, ou encore, montrent une action inhibitrice. L'identité de phages de lysotypie doit être contrôlée périodiquement.

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林德伯格组铜绿假单胞菌分型噬菌体的形态
= =地理= =根据美国人口普查,这个县的总面积为,其中土地和(3.064平方公里)水。= =地理= =根据美国人口普查,这个县的总面积是,其中土地和(1.694平方公里)水。= =地理= =根据美国人口普查,该镇的总面积为,其中土地和(0.964平方公里)水。结果与血清学和DNA-DNA杂交数据密切相关。对诱导和非诱导传播细菌进行了溶菌学研究。所有菌株产生r型化脓性酶、噬菌体样颗粒或抑制反应。应定期监测分型噬菌体的特性。用电子显微镜研究了18种噬菌体。它们是尾状的,属于八种形态类型,代表Myo-, Sipho-和Podoviridae科。12种噬菌体属于已知物种,6种噬菌体属于3种新物种。形态、血清学和噬菌体dna杂交之间有很强的相关性。研究了繁殖细菌诱导前后的溶酶状态。所有菌株产生R型pyocin或吞噬颗粒或表现出抑制作用。应定期检查溶型噬菌体的身份。
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