Yamadazyma oleae f.a. sp. nov. and Yamadazyma molendinolei f.a. sp. nov., two novel ascomycetous yeast species isolated from olive oil mills in Italy, and reassignment of 11 Candida species to the genus Yamadazyma.

IF 2 3区生物学 Q4 MICROBIOLOGY International journal of systematic and evolutionary microbiology Pub Date : 2024-12-01 DOI:10.1099/ijsem.0.006592

Michele Avesani, Giacomo Zapparoli, Sasitorn Jindamorakot, Savitree Limtong

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Abstract

Three yeast strains were isolated from an olive paste sample (CBS 18661) and two samples of olive oil (CBS 18662 and CBS 18660) were collected in two different mills in Verona province, Italy. The sequence comparison of the D1/D2 domains of the LSU rRNA gene and the internal transcribed space (ITS) regions indicated that these strains belonged to the genus Yamadazyma. Phylogenetic analysis of concatenated sequences of the ITS regions and the D1/D2 domains indicated that they represented members of two distinct species. Strain CBS 18661 and CBS 18662 showed sequence divergence of 2.7-2.8% (13-15 nucleotide substitutions) in the D1/D2 domains from the holotype of Yamadazyma luoyangensis, Yamadazyma ovata, Yamadazyma siamensis and Candida kanchanaburiensis and 5.9-6.2% identities (36-38 nucleotide substitutions) in the ITS regions from Yamadazyma akitaenisis and Yamadazyma nakazawae. Strain CBS 18660 showed sequence divergence of 0.75-1.1% (4-6 nucleotide substitutions) from Y. ovata, Candida trypodendri and Candida insectorum and 3.2-4.1% (18-24 nucleotide substitutions) in the ITS regions from Yamadazyma dushanensis, Yamadazyma terventina, Yamadazyma mexicana and C. trypodendri. The strains CBS 18661^T (PP391581-PP375117) and CBS 18662 (PP391582-PP375118) are named Yamadazyma oleae f.a. sp. nov., whereas CBS 18660^T (PP149061-PP130150) is assigned as Yamadazyma molendinolei f.a. sp. nov. The MycoBank numbers are MB 854683 and MB 854684, respectively. In addition, 11 Candida species belonging to the Yamadazyma clade have been reassigned to the genus Yamadazyma with the proposal of the following new combinations: Yamadazyma aaseri comb. nov., Yamadazyma conglobata comb. nov., Yamadazyma dendronema comb. nov., Yamadazyma diddensiae comb. nov., Yamadazyma germanica comb. nov., Yamadazyma insectorum comb. nov., Yamadazyma kanchanaburiensis comb. nov., Yamadazyma naeodendra comb. nov., Yamadazyma pseudoaaseri comb. nov., Yamadazyma trypodendri comb. nov. and Yamadazyma vaughaniae comb. nov.

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从意大利橄榄油厂分离的两个子囊菌新种——油酸山azyma f.a. sp. 11和molendinolei f.a. sp. 11，以及11种假丝酵母菌重新归属于山azyma属。

从意大利维罗纳省两家不同的工厂收集的橄榄油样品（CBS 18662和CBS 18660）和橄榄油样品（CBS 18662和CBS 18660）中分离出三株酵母菌。LSU rRNA基因D1/D2结构域与内部转录空间（ITS）区域的序列比较表明，这些菌株属于山azyma属。ITS区域和D1/D2结构域的系统发育分析表明，它们代表两个不同的物种。菌株CBS 18661和CBS 18662在D1/D2结构域与洛阳山azyma、ovata山azyma、siamma和kanchanaburiensis纯型的序列差异为2.7% -2.8%（13-15个核苷酸替换），在ITS区域与akitaenisis山azyma和nakazawaae山azyma的序列差异为5.9% -6.2%（36-38个核苷酸替换）。菌株CBS 18660的ITS区序列与ovata、trypodendri和昆虫假丝酵母的差异为0.75 ~ 1.1%（4 ~ 6个核苷酸替换），与dushanyamazyma、terventina Yamadazyma、mexicana Yamadazyma和trypodendri的差异为3.2 ~ 4.1%（18 ~ 24个核苷酸替换）。菌株CBS 18661T （PP391581-PP375117）和CBS 18662 （PP391582-PP375118）命名为Yamadazyma oleae f.a. sp. nov., CBS 18660T （PP149061-PP130150）命名为Yamadazyma molendinolei f.a. sp. nov.，菌库编号分别为MB 854683和MB 854684。此外，11种原属念珠菌（Yamadazyma）被重新划归到Yamadazyma属，并提出以下新组合：11月，山大和集团梳子。11月，山矢树突瘤梳。11月，山矢科梳子。11月，德国山芝梳子。11月，山矢虫梳。11月，Yamadazyma kanchanaburiensis梳子。11月，山齿菊梳。11月，山azyma pseudoaaseri梳子。11月，山竹梳。11月和山崎骏梳子。11月。

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来源期刊

International journal of systematic and evolutionary microbiology 生物-微生物学

CiteScore

5.20

自引率

21.40%

发文量

426

审稿时长

1 months

期刊介绍： Published by the Microbiology Society and owned by the International Committee on Systematics of Prokaryotes (ICSP), a committee of the Bacteriology and Applied Microbiology Division of the International Union of Microbiological Societies, International Journal of Systematic and Evolutionary Microbiology is the leading forum for the publication of novel microbial taxa and the ICSP’s official journal of record for prokaryotic names. The journal welcomes high-quality research on all aspects of microbial evolution, phylogenetics and systematics, encouraging submissions on all prokaryotes, yeasts, microfungi, protozoa and microalgae across the full breadth of systematics including: Identification, characterisation and culture preservation Microbial evolution and biodiversity Molecular environmental work with strong taxonomic or evolutionary content Nomenclature Taxonomy and phylogenetics.