Super signal-enhancement biosensing platform for precise target recognition based on rolling circle-hybridization chain dual linear cascade amplification technology.
Danyao Tang, Chunyuan Xiong, YeYu Wu, Hu Luo, Jun Yan, Ke-Jing Huang, Xuecai Tan, Yu Ya
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引用次数: 0
Abstract
This paper presents a self-powered biosensing platform based on graphdiyne@Au (2D GDY@Au) nanoparticles and rolling circle-hybridization chain (RC-HC) dual linear cascade amplification technology, which significantly enhances target recognition and signal amplification efficiency for miRNA-141. Specifically, the target on bioanode outputs a large amount of single-stranded DNA (T1) through the strand displacement amplification (SDA) mechanism. This efficient target recycling process triggers RC-HC dual linear cascade reaction. The RCA product and H2 form the L-Liner/H2 hybridized chain through a hybridization chain reaction, and then are immobilized on a flexible electrode using a Y-DNA capture handle. [Ru(NH3)6]3+ is precisely anchored in the grooves of the DNA double helix. The 2D GDY@Au enhances the electron mobility of the system to form a rich electron-donating center. The [Ru(NH3)6]3+ on the biocathode receives electrons and is reduced to [Ru(NH3)6]2+, producing a significantly amplified open-circuit voltage signal. Dual linear cascade amplification technology realizes precise target recognition, exponential amplification, and efficient conversion of biological signals. This technique displays an extensive linear range (0.0001-10000 pM) with a detection limit of 25.9 aM (S/N = 3), and it provides an innovative method for developing sensors based on nucleic acid amplification and presents a promising novel approach for the sensitive and precise detection of low-abundance target molecules, highlighting a new tactic for the creation of compact and portable analytical devices.
期刊介绍:
Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome.
Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.