{"title":"Optical detection based spot test on electrospun nanofibers for glioblastoma cells.","authors":"Aybuke Elif Us, Fatma Ozturk Kirbay, Ozge Kozgus Guldu, Emin Ilker Medine, Dilek Odaci","doi":"10.1016/j.talanta.2024.127303","DOIUrl":null,"url":null,"abstract":"<p><p>Paper-based diagnosis is enabled to be used in many applications in global health due to its low cost and simplicity of operation. Electrospun nanofibers (ESNFs) are useful platforms to prepare paper-based diagnostics. Herein, bead-free ESNFs were formed by electrospinning using polystyrene (PS) and poly (ethylene glycol) (PEG) polymers as a paper-based substrate. PS:PEG ESNFs were characterized by scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), identification of swelling behavior, and Brunauer-Emmett-Teller (BET) analysis. Synthesized gold nanoparticles (AuNPs) were used as a colorimetric probe and GMT8 aptamer was conjugated on the surface of AuNPs. AuNP and AuNP-GMT8 were characterized by dynamic light scattering (DLS), UV-Visible spectrometry, and X-Ray photoelectron spectroscopy (XPS). The interaction of AuNP-GMT8 bioconjugates with glioblastoma cells was examined. The colored spots for the mixture of glioblastoma (U87-MG) cells and AuNP-GMT8 were optically monitored both in the microplate wells and on the PS:PEG ESNFs. The linear range of colorimetric analysis on PS:PEG ESNFs was determined to be between 10<sup>1</sup>-10<sup>6</sup> U87-MG cells/mL by smartphone using RGB color analysis.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"285 ","pages":"127303"},"PeriodicalIF":5.6000,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Talanta","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1016/j.talanta.2024.127303","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/11/27 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
Abstract
Paper-based diagnosis is enabled to be used in many applications in global health due to its low cost and simplicity of operation. Electrospun nanofibers (ESNFs) are useful platforms to prepare paper-based diagnostics. Herein, bead-free ESNFs were formed by electrospinning using polystyrene (PS) and poly (ethylene glycol) (PEG) polymers as a paper-based substrate. PS:PEG ESNFs were characterized by scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), identification of swelling behavior, and Brunauer-Emmett-Teller (BET) analysis. Synthesized gold nanoparticles (AuNPs) were used as a colorimetric probe and GMT8 aptamer was conjugated on the surface of AuNPs. AuNP and AuNP-GMT8 were characterized by dynamic light scattering (DLS), UV-Visible spectrometry, and X-Ray photoelectron spectroscopy (XPS). The interaction of AuNP-GMT8 bioconjugates with glioblastoma cells was examined. The colored spots for the mixture of glioblastoma (U87-MG) cells and AuNP-GMT8 were optically monitored both in the microplate wells and on the PS:PEG ESNFs. The linear range of colorimetric analysis on PS:PEG ESNFs was determined to be between 101-106 U87-MG cells/mL by smartphone using RGB color analysis.
期刊介绍：
Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome.
Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.
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